Progenitors in prostate development and disease

Joseph, Diya B.; Turco, Anne E.; Vezina, Chad M.; Strand, Douglas W.

doi:10.1016/j.ydbio.2020.11.012

Cited by 17 publications

(24 citation statements)

References 90 publications

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“…We then examine the functional characterization of luminal progenitor cells and their involvement in prostate homeostasis and pathogenesis in mice and, potentially, in humans. Certain aspects of luminal progenitor biology (for example, epithelial lineage hierarchy, development and ageing) outside the scope of this Review are reviewed in detail elsewhere 11,37,38 .…”

Section: ();mentioning

confidence: 99%

“…To investigate the actual overlap between these cell clusters, we reanalysed the scRNA-seq transcriptomic data [32][33][34][35][36] to identify the top biomarkers of these putative luminal progenitor cells in each individual study and compare their expression level in each cell population across studies 50 . Although one study claimed that the non-secretory luminal cell cluster was actually of urethral origin 36,37 (see Spatial distribution), their scRNA-seq data were included in the comparison given the obvious molecular proximity of these 'urethral' luminal cells with the putative luminal progenitor cells (herein used as the generic term for this cluster) referred to as prostatic cells by all other groups [32][33][34][35] . This analysis identified a signature of 15 genes common to all studies (TaBle 3).…”

Section: Single-cell Profilingmentioning

confidence: 99%

“…As in mice, the luminal compartment of the human prostate displayed higher diversity than the basal cell compartment, as all but one study 33 identified a unique basal cell cluster. In addition to secretory luminal cells, pioneering research by one group identified two unprecedented luminal cell types that share molecular similarity with two pulmonary epithelial progenitor cell types called Club and Hillock cells 37,51 . The existence of Club and Hillock cell populations in the human prostate has also been reported in other studies 33,34 .…”

Section: ();mentioning

confidence: 99%

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Prostate luminal progenitor cells: from mouse to human, from health to disease

et al. 2022

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Stem and progenitor cells of the adult prostate epithelium have historically been believed to reside mainly or exclusively within the basal cell compartment and to possess basal-like phenotypic characteristics. Within the past decade, evidence of the existence of luminal epithelial cells exhibiting stem/progenitor properties has been obtained by lineage tracing and by functional characterization of sorted luminal-like cells. In 2020, the boom of single-cell transcriptomics led to increasingly exhaustive profiling of putative mouse luminal progenitor cells and, importantly, to the identification of cognate cells in the human prostate. The enrichment of luminal progenitor cells in genetically modified mouse models of prostate inflammation, benign prostate hypertrophy and prostate cancer, and the intrinsic castration tolerance of these cells, suggest their potential role in prostate pathogenesis and in resistance to androgen deprivation therapy. This Review bridges different approaches that have been used in the field to characterize luminal progenitor cells, including the unification of multiple identifiers employed to define these cells (names and markers). It also provides an overview of the intrinsic functional properties of luminal progenitor cells, and addresses their relevance in mouse and human prostate pathophysiology.

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Section: ();mentioning

confidence: 99%

Section: Single-cell Profilingmentioning

confidence: 99%

Section: ();mentioning

confidence: 99%

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Prostate luminal progenitor cells: from mouse to human, from health to disease

et al. 2022

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“…These findings have generated a particular interest for this particular cell subset (Joseph et al 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Luminal progenitors have recently emerged as key players of prostate pathogenesis including inflammation (Wang et al 2015), benign prostate hyperplasia (Crowell et al 2019, Joseph et al 2020) and prostate cancer (Korsten et al 2009, Wang et al 2014, Sackmann Sala et al 2017, Guo et al 2020). These findings have generated a particular interest for this particular cell subset (Joseph et al 2021). However, one recurrent issue in the stem cell field is to evaluate to which extent cell populations identified in different studies, often by different experimental approaches, actually correspond to equivalent cell entities.…”

Section: Discussionmentioning

confidence: 99%

In silico Computed Clusters of Prostate Luminal Progenitors Match FACS-Enriched LSC^med cells

Baurès

Dariane

et al. 2021

Preprint

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Several groups recently published single-cell (sc) expression atlases of the adult mouse prostate cells based on RNA sequencing (scRNA-seq) data. All studies identified one computerized cluster of non-secretory luminal progenitor cells enriched in luminal and stemness-related gene transcripts. The actual correspondence between these luminal progenitor cell clusters has not been investigated. In addition, the presence of Krt4 (encoding cytokeratin 4) in these in silico-identified luminal progenitors suggested the overlap with FACS-enriched LSCmed luminal progenitor cells earlier identified as a stem-like, castration-tolerant and tumor-initiating cell population. Here, we used a unified bioinformatics pipeline to re-analyze published prostate scRNA-seq datasets and perform various pan-transcriptomic comparisons including the LSCmed cell signature. Our study demonstrates that i) the mouse prostate luminal progenitor cell clusters identified in the different scRNA-seq studies largely overlap and can be defined by a common 15-gene signature including Krt4, ii) mouse LSCmed cells match both mouse and human luminal progenitors identified by scRNA-seq analysis. Bridging these in silico-identified and ex vivo-characterized prostate luminal progenitor subsets should benefit our understanding of their actual involvement in prostate diseases.

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Lung patterning: Is a distal‐to‐proximal gradient of cell allocation and fate decision a general paradigm?

Zhang,

Aung,

Yao

et al. 2023

BioEssays

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Recent studies support a model in which the progeny of SOX9+ epithelial progenitors at the distal tip of lung branches undergo cell allocation and differentiation sequentially along the distal‐to‐proximal axis. Concomitant with the elongation and ramification of lung branches, the descendants of the distal SOX9+ progenitors are distributed proximally, express SOX2, and differentiate into cell types in the conducting airways. Amid subsequent sacculation, the distal SOX9+ progenitors generate alveolar epithelial cells to form alveoli. Sequential cell allocation and differentiation are integrated with the branching process to generate a functional branching organ. This review focuses on the roles of SOX9+ cells as precursors for new branches, as the source of various cell types in the conducting airways, and as progenitors of the alveolar epithelium. All of these processes are controlled by multiple signaling pathways. Many mouse mutants with defective lung branching contain underlying defects in one or more steps of cell allocation and differentiation of SOX9+ progenitors. This model provides a framework to understand the molecular basis of lung phenotypes and to elucidate the molecular mechanisms of lung patterning. It builds a foundation on which comparing and contrasting the mechanisms employed by different branching organs in diverse species can be made.

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Progenitors in prostate development and disease

Cited by 17 publications

References 90 publications

Prostate luminal progenitor cells: from mouse to human, from health to disease

Prostate luminal progenitor cells: from mouse to human, from health to disease

In silico Computed Clusters of Prostate Luminal Progenitors Match FACS-Enriched LSC^med cells

Lung patterning: Is a distal‐to‐proximal gradient of cell allocation and fate decision a general paradigm?

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Progenitors in prostate development and disease

Cited by 17 publications

References 90 publications

Prostate luminal progenitor cells: from mouse to human, from health to disease

Prostate luminal progenitor cells: from mouse to human, from health to disease

In silico Computed Clusters of Prostate Luminal Progenitors Match FACS-Enriched LSCmed cells

Lung patterning: Is a distal‐to‐proximal gradient of cell allocation and fate decision a general paradigm?

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In silico Computed Clusters of Prostate Luminal Progenitors Match FACS-Enriched LSC^med cells