Progesterone and norgestrel alter transcriptional expression of genes along the hypothalamic–pituitary–thyroid axis in zebrafish embryos-larvae

Liang, Yan‐Qiu; Huang, Guo-Yong; Ying, Guang‐Guo; Liu, Shuangshuang; Jiang, Yu-Xia; Liu, Shan

doi:10.1016/j.cbpc.2014.09.007

Cited by 22 publications

(14 citation statements)

References 45 publications

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“…Dio1 is a difunctionality deiodinase as it is capable of promoting both activation and inactivation of THs. However, Dio2 only catalyzes activation and Dio3 merely the middle of catalyzes inactivation [ 22 ]. The main effect of D io3 is to deactivate T4 by inner ring deiodination (IRD), leading to the formation of the inactive 3,3′,5′-triiodothyronine (rT3) or to generate 3,3′-diiodothyronine (T2) from T3.…”

Section: Introductionmentioning

confidence: 99%

Thyroid Disruption in Zebrafish Larvae by Short-Term Exposure to Bisphenol AF

Tang

Yang

Chen

et al. 2015

IJERPH

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Bisphenol AF (BPAF) is extensively used as a raw material in industry, resulting in its widespread distribution in the aqueous environment. However, the effect of BPAF on the hypothalamic-pituitary-thyroidal (HPT) axis remains unknown. For elucidating the disruptive effects of BPAF on thyroid function and expression of the representative genes along the HPT axis in zebrafish (Danio rerio) embryos, whole-body total 3,3′,5-triiodothyronine (TT3), total 3,5,3′,5′-tetraiodothyronine (TT4), free 3,3′,5-triiodothyronine (FT3) and free 3,5,3′,5′-tetraiodothyronine (FT4) levels were examined following 168 h post-fertilization exposure to different BPAF concentrations (0, 5, 50 and 500 μg/L). The results showed that whole-body TT3, TT4, FT3 and FT4 contents decreased significantly with the BPAF treatment, indicating an endocrine disruption of thyroid. The expression of thyroid-stimulating hormone-β and thyroglobulin genes increased after exposing to 50 μg/L BPAF in seven-day-old larvae. The expressions of thyronine deiodinases type 1, type 2 and transthyretin mRNAs were also significantly up-regulated, which were possibly associated with a deterioration of thyroid function. However, slc5a5 gene transcription was significantly down-regulated at 50 μg/L and 500 μg/L BPAF exposure. Furthermore, trα and trβ genes were down-regulated transcriptionally after BPAF exposure. It demonstrates that BPAF exposure triggered thyroid endocrine toxicity by altering the whole-body contents of thyroid hormones and changing the transcription of the genes involved in the HPT axis in zebrafish larvae.

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Section: Introductionmentioning

confidence: 99%

Thyroid Disruption in Zebrafish Larvae by Short-Term Exposure to Bisphenol AF

Tang

Yang

Chen

et al. 2015

IJERPH

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“…Exposure to BPA and BPA analogues further induced transcription of genes involved in TH metabolism, i.e., Dio1 and/or Dio2 [37,129,132,134], which are implicated in activation/inactivation of T4 and in conversion of T4 to T3 in peripheral tissues, respectively [136,137], and Ugt1ab [37,38,129].…”

Section: Zebrafishmentioning

confidence: 99%

Bisphenols as Environmental Triggers of Thyroid Dysfunction: Clues and Evidence

Gorini

Bustaffa

Coi

et al. 2020

IJERPH

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Bisphenols (BPs), and especially bisphenol A (BPA), are known endocrine disruptors (EDCs), capable of interfering with estrogen and androgen activities, as well as being suspected of other health outcomes. Given the crucial role of thyroid hormones and the increasing incidence of thyroid carcinoma in the last few decades, this review analyzes the effects of BPS on the thyroid, considering original research in vitro, in vivo, and in humans published from January 2000 to October 2019. Both in vitro and in vivo studies reported the ability of BPs to disrupt thyroid function through multiple mechanisms. The antagonism with thyroid receptors (TRs), which affects TR-mediated transcriptional activity, the direct action of BPs on gene expression at the thyroid and the pituitary level, the competitive binding with thyroid transport proteins, and the induction of toxicity in several cell lines are likely the main mechanisms leading to thyroid dysfunction. In humans, results are more contradictory, though some evidence suggests the potential of BPs in increasing the risk of thyroid nodules. A standardized methodology in toxicological studies and prospective epidemiological studies with individual exposure assessments are warranted to evaluate the pathophysiology resulting in the damage and to establish the temporal relationship between markers of exposure and long-term effects.

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“…This progestin, affecting the first step of the synthesis of thyroid hormones, seems also to exert an effect in the hypothalamic-pituitary-thyroid axis. Indeed, norgestrel induced the gene expression of the sodium/iodide symporter that is responsible for the intake of iodide anion into the follicular cells (Liang et al 2015c). DRSP affects central functions in zebrafish brain, disturbing the circadian rhythm network (Zucchi et al 2014, Zhao et al 2015b.…”

Section: Environmental Effects In Aquatic Lifementioning

confidence: 99%

The other side of progestins: effects in the brain

Giatti

Melcangi

Pesaresi

2016

Journal of Molecular Endocrinology

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Progestins are a broad class of progestational agents widely differing in their chemical structures and pharmacological properties. Despite emerging data suggest that progestins, besides their action as endometrial protection, can also have multiple nonreproductive functions, much remains to be discovered regarding the actions exerted by these molecules in the nervous system. Here, we report the role exerted by different progestins, currently used for contraception or in postmenopausal hormone replacement therapies, in regulating cognitive functions as well as social behavior and mood. We provide evidence that the effects and mechanisms underlying their actions are still confusing due to the use of different estrogens and progestins as well as different doses, duration of exposure, route of administration, baseline hormonal status and age of treated women. We also discuss the emerging issue concerning the relevant increase of these substances in the environment, able to deeply affect aquatic wildlife as well as to exert a possible influence in humans, which may be exposed to these compounds via contaminated drinking water and seafood. Finally, we report literature data showing the neurobiological action of progestins and in particular their importance during neurodegenerative events. This is extremely interesting, since some of the progestins currently used in clinical practice exert neuroprotective and anti-inflammatory effects in the nervous system, opening new promising opportunities for the use of these molecules as therapeutic agents for trauma and neurodegenerative disorders.

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Progesterone and norgestrel alter transcriptional expression of genes along the hypothalamic–pituitary–thyroid axis in zebrafish embryos-larvae

Cited by 22 publications

References 45 publications

Thyroid Disruption in Zebrafish Larvae by Short-Term Exposure to Bisphenol AF

Thyroid Disruption in Zebrafish Larvae by Short-Term Exposure to Bisphenol AF

Bisphenols as Environmental Triggers of Thyroid Dysfunction: Clues and Evidence

The other side of progestins: effects in the brain

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