Progesterone and Related Steroids in the Blood of Domestic Animals

Short, R. V.

doi:10.1002/9780470719046.ch21

Cited by 5 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Routine blood and urine tests, as well as sampling animal saliva, are generally used to screen for their presence. However, analysis of these controlled substances in bodily fluids normally provide ‘short‐term' exposure values, as these chemicals can readily be removed from the circulation (to a large extent) within hours, though detection of metabolites in urine is possible for up to 2–6 days . Therefore, it may also be of value to have a sense of “long‐term” or chronic exposure of these drugs in racing animals.…”

Section: Introductionmentioning

confidence: 99%

Analysis of illicit glucocorticoid levels in camel hair using competitive ELISA – Comparison with LC–MS/MS

Ashraf

El‐Gahany

Alraeesi

et al. 2020

Drug Testing and Analysis

View full text Add to dashboard Cite

Accurate, sensitive, and rapid screening of performance‐enhancing drugs, including glucocorticoids, is critical to combat doping in animal racing sports. Samples of urine, saliva, and serum are usually used for random screening of controlled substances; however, they tend to provide only acute usage and doping information. Hair testing has the potential to detect long‐term drug use in racing animals. In the present study, commercially available ELISA‐kits were used to rapidly screen and analyze various natural and synthetic glucocorticoids in the hair of camels. The four glucocorticoids that were screened using the competitive ELISA were hydrocortisone, dexamethasone, flumethasone, and methylprednisolone. However, comparison of the results obtained using the ELISA method with our previously published LC–MS/MS assay did not show a good correlation. The results obtained with the ELISA analysis of hair samples of 27 different camels showed that this technique consistently yielded significantly higher levels of glucocorticoids compared with the LC–MS/MS assay. This is an interesting finding and suggests that commercially available ELISA tests may overestimate the amount of glucocorticoids present in camel hair, perhaps due to specificity and cross‐reactivity issues.

show abstract

Section: Introductionmentioning

confidence: 99%

Analysis of illicit glucocorticoid levels in camel hair using competitive ELISA – Comparison with LC–MS/MS

Ashraf

El‐Gahany

Alraeesi

et al. 2020

Drug Testing and Analysis

View full text Add to dashboard Cite

show abstract

“…Recovery values ranged from 74.7 to 80.0 percent. Edgar (1953) reported an average recovery of 75 percent and Short (1957) reported a 50 percent recovery of progesterone added to extracts.…”

Section: Morphological Changes Ixiring Pregnancy Of the Cowmentioning

confidence: 99%

“…Pregnancy has been shown to continue in the guinea pig, mare, cow, ewe, man and monkey with removal of the corpora lutea after nid pregnancy; Baeside end Turner ( 1951 )î Uren andRaeside (1951): Mc Donald, Nichols, andMc Nutt (1953); end Short (1957). genterone might be needed for parturition.…”

Section: Introductionmentioning

confidence: 99%

Progesterone content of bovine reproductive organs during pregnancy

Rakes

View full text Add to dashboard Cite

“…During pregnancy the first maximum amount of progestin was reached at 20 days with a further increase to 105 days. (1953); end Short (1957). genterone might be needed for parturition.…”

Section: Historicalmentioning

confidence: 99%

Progesterone content of bovine reproductive organs during pregnancy

Rakes

View full text Add to dashboard Cite

The stationary phase was formed by water bound to the cellulose fibers on filter paper. The mobile phase was an organic solvent immiscible with EgC.These workers were credited with working out many of the basic problems on chromatography of steroids using a petroleum ether; propylene glycol system known as the Zafferoni system. Bush (1953 ) had developed the system using petroleum ether: methanol: water which was known as the orthodox method with volatile solvents.

show abstract

Progesterone and Related Steroids in the Blood of Domestic Animals

Cited by 5 publications

References 18 publications

Analysis of illicit glucocorticoid levels in camel hair using competitive ELISA – Comparison with LC–MS/MS

Analysis of illicit glucocorticoid levels in camel hair using competitive ELISA – Comparison with LC–MS/MS

Progesterone content of bovine reproductive organs during pregnancy

Progesterone content of bovine reproductive organs during pregnancy

Contact Info

Product

Resources

About