Progesterone Concentration in Peripheral Blood Plasma of the Domestic Sow Prior to and during Early Pregnancy

Sa, Tillson; Re, Erb

doi:10.2527/jas1967.2661366x

Cited by 13 publications

(4 citation statements)

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“…Patterns of plasma progesterone during the oestrous cycle are in agreement with those reported by other workers (Tillson & Erb, 1967;Stabenfeldt et al, 1969;Edqvist & Lamm, 1971), with the exception that the oestrous values (2 to 5 ng/ml) are somewhat higher than those (1 ng/ml) recorded by Stabenfeldt et al (1969). The results of three assays for Pig 375 carried out at different times suggest that repeated thawing and refreezing of plasma may have a small but detrimental effect on progesterone.…”

Section: Hormone Determinations In Pig Plasmasupporting

confidence: 90%

Peripheral Plasma Progesterone and Oestradiol-17 Levels Before and After Puberty in Gilts

Shearer¹,

Purvis²,

Jenkin³

et al. 1972

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A double isotope derivative assay and a simple competitive protein-binding assay were used to measure peripheral plasma progesterone levels during the pig oestrous cycle. In view of the similarity between the results, the more convenient protein-binding method was employed to determine progesterone levels during prepuberty, the oestrous cycle, early and late pregnancy, parturition and lactation. Peripheral plasma oestradiol-17\g=b\ levels were measured during the oestrous cycle, early pregnancy and around the time of parturition.

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Section: Hormone Determinations In Pig Plasmasupporting

confidence: 90%

Peripheral Plasma Progesterone and Oestradiol-17 Levels Before and After Puberty in Gilts

Shearer¹,

Purvis²,

Jenkin³

et al. 1972

Reproduction

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“…the fact that no correction has been made for the blank values. The high values during the follicular phase reported by Tillson & Erb (1967) are probably due to excess of tritiated products not associated with progesterone, which will give an over-estimation of progesterone. The values found during the luteal phase are in very good agreement with those obtained by Stabenfeldt et al (1969).…”

mentioning

confidence: 94%

Progesterone Levels in Plasma During the Oestrous Cycle of the Sow Measured by a Rapid Competitive Protein Binding Technique

Edqvist¹,

Lamm²

1971

Reproduction

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An adaptation of the technique described by Johansson (1969), whereby progesterone was measured by a rapid competitive protein binding technique, has been used to determine the progesterone levels in the peripheral plasma of the cow (Edqvist, Ekman, Gustafsson & \ l =A%o\ st r\ l =o"\ m, 1970). The purpose of this paper is to report the usefulness of the same technique for the measurement of progesterone levels in the peripheral blood plasma of the sow during the oestrus cycle. Two sows of the Swedish Landrace Breed, fed on a commercial pig diet with unlimited water supply, were used. Sexual receptivity was checked twice a day with a boar. The first day of receptivity was considered to be the first day of the oestrous cycle. About 1 ml of blood was collected daily from an ear vein into heparinized tubes. Plasma was removed after centrifugation and stored at-15\s=deg\C until assayed. A total of sixty-eight blood samples were analysed in duplicate, each determination requiring 0\m=.\1ml of plasma. The basic steps of the rapid competitive protein binding technique (Johansson, 1969; Edqvist et al., 1970) include (1) extraction of the plasma with petroleum ether, (2) evaporation of the extract, (3) binding reaction, (4) separation of bound and unbound fractions and (5) radioactive counting of the bound fraction. A standard-curve was processed simultaneously for each set of samples analysed. Corticosterone-1,2-3H (obtained from New England Nuclear, Boston, Mass., U.S.A.) with a specific activity of 50 Ci/mm was used as tracer and domestic fowl plasma as assay protein. The plasma progesterone levels during the sexual cycle in two animals are presented in Text-fig. 1. An initial rise occurs on Days 3 and 4 of the cycle. Peak luteal progesterone levels (28 to 37 ng/ml) were obtained on Days 13 and 14. Minimum values were observed during the follicular and ovulatory phases (2\m=.\5ng/ml). This period lasts for about 6 days. Tillson & Erb (1967), using a double isotope derivative technique, found about 7 ng of progesterone/ml plasma at oestrus, and peak levels of about 25 ng/ml plasma occurred on Day 10 of the cycle. Stabenfeldt, Akins, Ewing & Morrissette (1969), using gas-liquid chromatography with electron-capture detection, found values of about 0-5 ng/ml at oestrus and 35 ng/ml plasma at

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“…7.674 1\41-' 6.41.4e 10%.,., luteal phase as a starting point for a consideration of cyclic control seems logical. The changes in peripheral progesterone concentrations are the best documented endocrine events of the pig oestrous cycle (Tillson and Erb, 1967;Stabenfeldt et at, 1969;Tillson, Erb and Niswender, 1970;Edquist and Lamm, 1971;Shearer et al, 1972;Henricks, Guthrie and Handlin, 1972;Parvizi et at, 1976;Van de Wiel et at, 1981), although some inconsistencies still exist in these data. As shown in Figure 8.1(a), a rise in progesterone secretion is generally observed on days 3-4 of the oestrous cycle (the first day of standing heat being designated as day 0), but considerable variability exists as to the time of maximum circulating concentrations.…”

Section: The Established Luteal 'Phase Of the Cyclementioning

confidence: 99%

Endocrine Control of The Oestrous Cycle

Foxcroft

Van²

2020

Proc

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Progesterone Concentration in Peripheral Blood Plasma of the Domestic Sow Prior to and during Early Pregnancy

Cited by 13 publications

References 0 publications

Peripheral Plasma Progesterone and Oestradiol-17 Levels Before and After Puberty in Gilts

Peripheral Plasma Progesterone and Oestradiol-17 Levels Before and After Puberty in Gilts

Progesterone Levels in Plasma During the Oestrous Cycle of the Sow Measured by a Rapid Competitive Protein Binding Technique

Endocrine Control of The Oestrous Cycle

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