Progesterone in vitro stimulates secretion of a specific protein by ovine epithelial endometrial cells

Salamonsen, Lois A.; Healy, David; Findlay, J. K.

doi:10.1016/0022-4731(87)91020-x

Cited by 8 publications

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Sex hormone receptors in gastric cancer

Chi

Chang

et al. 1990

Cancer

View full text Add to dashboard Cite

Gastric adenocarcinoma that originates from mucosal tissue invades submucosa, muscle, and serosa in different stages. The level of progesterone receptors (PgR), estrogen receptors (ER), and androgen receptors (AdR) in the superficial part of gastric cancer tissues (CAs) from 16 patients was determined and compared with that of the corresponding normal gastric mucosal tissues (NLm). There were PgR in all CAs (100%) with values that ranged from 20.5 to 548.4 fmol/mg protein. Eight CAs (50%) had ER values that ranged from 6.8 to 325.1 fmol/mg protein. AdR was found in two CAs with values of 14.7 and 16.4 fmol/mg protein. In NLm, 15 (93.8%) had PgR values that ranged from 7.3 to 473.2 fmol/mg protein and ten (62.5%) had ER values that ranged from 0.9 to 87.9 fmol/mg protein. AdR were present in two NLm with values of 1.5 and 73.5 fmol/mg protein. There was no statistical difference in levels of PgR and ER between CAs and NLm. There were PgR in all gastric cancers and in 93.8% of NLm. The results suggest that gastric mucosa may be the target tissues for progesterone action. Furthermore, the lack of correlation between the levels of ER and PgR in gastric cancer tissue suggests that the PgR in gastric cancers are probably estrogen independent.

show abstract

Sex hormone receptors in gastric cancer

Chi

Chang

et al. 1990

Cancer

View full text Add to dashboard Cite

show abstract

An In Vitro Model for Studying Interactions Between Mouse Trophoblast and Uterine Epithelial Cells

Morris

Potter

1990

Trophoblast Invasion and Endometrial Receptivity

View full text Add to dashboard Cite

Effect of progesterone on protein synthesis and secretion by cultured epithelial cells from guinea-pig endometrium

et al. 1989

View full text Add to dashboard Cite

The pattern of proteins synthesized and secreted in response to progesterone by guinea-pig endometrial epithelial cell cultured with estradiol-17 beta was investigated. Glandular epithelial cells were maintained in culture for 3 days on growth medium, then washed three times with a steroid-free medium. After this period, 2 x 10(-8) M estradiol-17 beta or 2 x 10(-8) M estradiol-17 beta plus 5 x 10(-7) M progesterone were added to the medium for 48 h. To study biochemical changes, the proteins in medium and in cells were analyzed by one- and two-dimensional polyacrylamide gel electrophoresis, followed by fluorography. The addition of progesterone to estradiol-17 beta in the culture medium caused a change in the patterns of cellular and secreted proteins: one-dimensional gel electrophoresis showed that variation of 8 cellular proteins and 12 secreted proteins was caused by progesterone. Induction of individual proteins by progesterone treatment was observed by two-dimensional gel electrophoresis: one cellular protein (Mr 49,000; pI 5.90) and one secreted protein (Mr 14,300; pI 4.80) were specifically induced and might serve as markers of progesterone action.

show abstract

Progesterone in vitro stimulates secretion of a specific protein by ovine epithelial endometrial cells

Cited by 8 publications

References 15 publications

Sex hormone receptors in gastric cancer

Sex hormone receptors in gastric cancer

An In Vitro Model for Studying Interactions Between Mouse Trophoblast and Uterine Epithelial Cells

Effect of progesterone on protein synthesis and secretion by cultured epithelial cells from guinea-pig endometrium

Contact Info

Product

Resources

About