Progesterone, LH, Estrus and Ovulation after Prostaglandin F2  in Heifers

Louis, Thomas M.; Hafs, H. D.; Seguin, B.E.

doi:10.3181/00379727-143-37274

Cited by 116 publications

(37 citation statements)

References 5 publications

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“…Therefore, it is likely that PGE 2 synthesized by the luteal cells in this culture system can also inhibit luteal cell-stimulated T lymphocyte proliferation. However, previous studies [12,[23][24][25][26] have demonstrated an inhibitory effect of progesterone on lymphocyte proliferation in several species, including the cow, and in the present study, exogenous progesterone does-dependently inhibited luteal cell-stimulated T lymphocyte proliferation. The inhibitory effect of progesterone on T lymphocyte proliferation in the present study does not appear to be mediated via progesterone receptors in T lymphocytes, because progesterone-receptor mRNA was not detected by RT-PCR in total RNA isolated from PBMCs, which are rich in T lymphocytes.…”

Section: Discussioncontrasting

confidence: 48%

“…Twenty-five milligrams of PGF 2␣ (Lutalyse; Upjohn Co., Kalamazoo, MI) were administered i.m. to cows in the midluteal phase of the estrous cycle to induce luteal regression [12]. Luteal tissue and jugular venous blood were collected from cows before and 8 h after administration of a luteolytic dose of PGF 2␣ .…”

Section: Collection and Dissociation Of Luteal Tissuementioning

confidence: 99%

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Effects of Prostaglandin F2α and Progesterone on the Ability of Bovine Luteal Cells to Stimulate T Lymphocyte Proliferation1

Cannon

Petroff

Pate

2003

Biology of Reproduction

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Bovine luteal cells express class I and II major histocompatibility complex molecules and stimulate T lymphocyte proliferation in vitro. Proliferation of T lymphocytes is greater in cocultures of luteal cells and T lymphocytes collected following administration of a luteolytic dose of prostaglandin (PG) F2alpha to the cow. Whether this results from changes in luteal cells that increase their ability to stimulate T lymphocyte proliferation or from changes in T lymphocytes that enhance their ability to respond to luteal cells is unclear. To determine which is the case, luteal cell-T lymphocyte cocultures were performed using luteal cells and T lymphocytes isolated from the same animals before and 8 h after administration of PGF2alpha. In the presence of T lymphocytes collected before PGF2alpha administration, luteal cells isolated after PGF2alpha were more potent stimulators of T lymphocyte proliferation than were luteal cells collected before PGF2alpha (P<0.05). The effect of progesterone on luteal cell-stimulated T lymphocyte proliferation was also evaluated. Proliferation of T lymphocytes was greater (P<0.05) in cultures containing the cytochrome P450 side-chain cleavage enzyme-inhibitor aminoglutethimide. Exogenous progesterone caused a dose-dependent inhibition of luteal cell-stimulated T lymphocyte proliferation (P<0.05). Progesterone-receptor mRNA was undetectable in peripheral blood mononuclear cells collected before and after PGF2alpha administration, indicating that the effect of progesterone was not mediated via progesterone receptors in lymphocytes. These results imply that specific changes in luteal cells in response to PGF2alpha enhance the ability of these cells to stimulate T lymphocyte proliferation. These results also demonstrate that progesterone can suppress luteal cell-stimulated T lymphocyte proliferation.

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Section: Discussioncontrasting

confidence: 48%

Section: Collection and Dissociation Of Luteal Tissuementioning

confidence: 99%

Effects of Prostaglandin F2α and Progesterone on the Ability of Bovine Luteal Cells to Stimulate T Lymphocyte Proliferation1

Cannon

Petroff

Pate

2003

Biology of Reproduction

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“…assay was a modification of that described by Louis et al (1973). Duplicate 0.2-mL aliquots of serum were extracted twice with 5 mL of isooctane, the extract being decanted into tubes containing 2l pg radioactive P..…”

Section: Radioimmunoassaysmentioning

confidence: 99%

Effect of Forced Exercise During Gestation on Reproductive Performance of Sows

Ferket¹,

Hacker²

1985

Can. J. Anim. Sci.

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“…During the oestrous cycle in cattle, more large antral follicles are observed in the ovary bearing the corpus luteum (CL) than in the contralateral ovary (Pierson & Ginther, 1987a). In contrast, follicular growth in the CL-bearing ovary is decreased during early and mid-gestation (Rexroad & Casida, 1975;Thatcher et ai, 1986;Pierson & Ginther, 1987b;Guilbault et al, 1988) due to an increased rate of atresia (Guilbault et al, 1986 (Louis et al, 1973) and plasma oestradiol was measured after extraction (1-5-2-Oml plasma) with a mixture (5 ml) of petroleum ether:ethyl acetate (5-5:1 v/v). Recovery of labelled steroids after extraction was 89 + 0-8% for progesterone and 88 ± 1-2% for oestradiol.…”

Section: Introductionmentioning

confidence: 99%