Progesterone Receptor as an Indicator of Sperm Function

Gadkar, Sushama; Shah, Chirag; Sachdeva, Geetanjali; Samant, Urmila; Puri, Chander P

doi:10.1095/biolreprod67.4.1327

Cited by 35 publications

(27 citation statements)

References 54 publications

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“…32,33,35 Staining of mouse, stallion, and human spermatozoa with cellimpermeable fluorescein-tagged progesterone-BSA complex (P-FITC-BSA) revealed that progesterone receptors are masked by coating factors in freshly ejaculated sperm and become available after incubation under capacitating conditions, but only in a limited proportion of male gametes. 39,51,52 Similarly, in the present study, we obtained positive staining of progesterone receptors just for a fraction of sperm derived from B10.BR and B10.BR-Y del males. The sperm cells that were negative for P-FITC-BSA staining lack progesterone receptors or alternatively receptors in their membranes remained covered because of inefficient passing through the capacitating process.…”

Section: Discussionsupporting

confidence: 87%

Increased Progesterone Production in Cumulus—Oocyte Complexes of Female Mice Sired by Males With the Y-Chromosome Long Arm Deletion and its Potential Influence on Fertilization Efficiency

et al. 2015

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It was revealed previously that B10.BR(Y del ) females sired by males with the Y-chromosome long arm deletion differ from genetically identical B10.BR females sired by males with the intact Y chromosome. This is interpreted as a result of different epigenetic information which females of both groups inherit from their fathers. In the following study, we show that cumulus-oocyte complexes ovulated by B10.BR(Y del ) females synthesize increased amounts of progesterone, which is important sperm stimulator. Because their extracellular matrix is excessively firm, the increased progesterone secretion belongs presumably to factors that compensate this feature enabling unchanged fertilization ratios. Described compensatory mechanism can act only on sperm of high quality, presenting proper receptors. Indeed, low proportion of sperm of Y del males that poorly fertilize B10.BR(Y del ) oocytes demonstrates positive staining of membrane progesterone receptors. This proportion is significantly higher for sperm of control males that fertilize B10.BR(Y del ) and B10.BR oocytes with the same efficiency.

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Section: Discussionsupporting

confidence: 87%

Increased Progesterone Production in Cumulus—Oocyte Complexes of Female Mice Sired by Males With the Y-Chromosome Long Arm Deletion and its Potential Influence on Fertilization Efficiency

et al. 2015

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“…Expression of progesterone receptor (PR) was observed on human sperms, and a strong correlation between PR expression and sperm function has been demonstrated 71 . Intriguingly, unlike estrogens, progesterone promotes the capacitated sperm to undergo acrosomal reaction 72 , 73 .…”

Section: Mechanisms Of Action On Spermatidsmentioning

confidence: 99%

Ginseng and male reproductive function

Leung

Wong

2013

Spermatogenesis

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Ginseng is often referred to as the King of all herbs, and is found to be a promising agent to improve general well-being. Ginseng has also been reputed as an aphrodisiac, and is used to treat sexual dysfunction as well as to enhance sexual behavior in traditional Chinese medical practices. Data from animal studies have shown a positive correlation among ginseng, libido, and copulatory performances, and these effects have been confirmed in case-control studies in human. In addition, ginseng is found to improve the sperm quality and count of healthy individuals as well as patients with treatment-related infertility. These actions are mostly attributed to ginsenosides, the major pharmacological active components of ginseng. This review compiles the current knowledge about the multifaceted effects of ginseng on male reproductive function, and also focuses on its mechanisms of action that may represent novel therapeutic strategies for the treatment of male reproductive diseases or disorders.

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“…Progesterone binds to the acrosome region of the sperm head (Gadkar et al 2002, Noguchi et al 2008Fig. 8Ac and Ad), although fluorescence of the middle piece of the sperm flagellum appears to be autofluorescence (Noguchi et al 2008;Fig.…”

Section: Binding Of 17be 2 To Hamster Spermatozoamentioning

confidence: 99%

“…Although spermatozoa do not have a classic intracellular progesterone receptor (PGR), a PGR is present in the cell membrane (Sabeur et al 1996, Baldi et al 1998, 2009, Lösel & Wehling 2003, Luconi et al 2004, Jang & Yi 2005. Moreover, it has been suggested that progesterone binds to the acrosome region, and PGR is localized to the same region in human (Gadkar et al 2002) and hamster spermatozoa (Noguchi et al 2008). Phospholipase C (PLC; Fukami et al 2003) and/or protein kinase A (Harrison et al 2000) are involved in progesteroneinduced AR.…”

Section: Introductionmentioning

confidence: 99%

Suppression of progesterone-enhanced hyperactivation in hamster spermatozoa by estrogen

Fujinoki¹

2010

Reproduction

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In this study, I examined whether sperm hyperactivation in hamster is regulated by steroid hormones such as estrogen (estradiol, E 2 ) and progesterone. Although sperm hyperactivation was enhanced by progesterone, 17b-estradiol (17bE 2 ) itself did not affect sperm hyperactivation. However, 17bE 2 suppressed progesterone-enhanced hyperactivation in a concentration-dependent manner through non-genomic pathways when spermatozoa were exposed to 17bE 2 at the same time or before exposure to progesterone. When spermatozoa were exposed to 17bE 2 after exposure to progesterone, 17bE 2 did not suppress progesterone-enhanced hyperactivation. Moreover, 17a-estradiol, an inactive isomer of E 2 , did not suppress progesterone-enhanced hyperactivation. Observations using a FITC-conjugated 17bE 2 showed that it binds to the acrosome region of the sperm head. Binding of 17bE 2 to spermatozoa was not inhibited by progesterone, although 17bE 2 did not suppress progesterone-enhanced hyperactivation when spermatozoa were exposed to 17bE 2 after exposure to progesterone. On the other hand, binding of progesterone to spermatozoa was also not inhibited by 17bE 2 even if progesterone-enhanced hyperactivation was suppressed by 17bE 2 . Although tyrosine phosphorylations of sperm proteins were enhanced by progesterone, enhancement of tyrosine phosphorylations by progesterone was suppressed by 17bE 2 . Moreover, tyrosine phosphorylations were inhibited by 17bE 2 when only 17bE 2 was added to the medium. From these results, it is likely that 17bE 2 competitively suppresses progesterone-enhanced hyperactivation through the inhibition of tyrosine phosphorylations via non-genomic pathways.

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Progesterone Receptor as an Indicator of Sperm Function

Cited by 35 publications

References 54 publications

Increased Progesterone Production in Cumulus—Oocyte Complexes of Female Mice Sired by Males With the Y-Chromosome Long Arm Deletion and its Potential Influence on Fertilization Efficiency

Increased Progesterone Production in Cumulus—Oocyte Complexes of Female Mice Sired by Males With the Y-Chromosome Long Arm Deletion and its Potential Influence on Fertilization Efficiency

Ginseng and male reproductive function

Suppression of progesterone-enhanced hyperactivation in hamster spermatozoa by estrogen

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