Progesterone sperm chemoattraction may be modulated by its corticosteroid-binding globulin carrier protein

Teves, María E.; Guidobaldi, Héctor Alejandro; Uñates, Diego Rafael; Sánchez, Raúl; Miska, W.; Giojalas, Laura Cecilia

doi:10.1016/j.fertnstert.2009.09.012

Cited by 17 publications

(9 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This last stage in the route to the oocyte could be stimulated by a progesterone gradient due to a higher hormone concentration in the inner part of the cumulus (Teves et al 2006(Teves et al , 2009, by a different attractant (Eisenbach & Giojalas 2006) and/or by another mechanism (thermotaxis? ).…”

Section: Working Model To Explain Sperm Transport and Retention At Thmentioning

confidence: 99%

Sperm transport and retention at the fertilization site is orchestrated by a chemical guidance and oviduct movement

Guidobaldi

Teves²,

Uñates³

et al. 2012

Reproduction

View full text Add to dashboard Cite

In mammals, only a few spermatozoa arrive at the fertilization site. During the last step in the journey to the egg, apart from their selfpropulsion, spermatozoa may be assisted by oviduct movement and/or a guidance mechanism. The proportion of rabbit spermatozoa that arrive at the fertilization site was determined under in vivo conditions, in which either the ovulation products (secreting chemoattractants) and/or the oviduct movement (causing the displacement of the oviductal fluid) was inhibited. When only one of these components was inhibited, sperm transport to the fertilization site was partially reduced. However, when both the ovulation products and the oviduct movement were inhibited, almost no spermatozoa arrived at the fertilization site. The results suggest that spermatozoa are transported to and retained at the fertilization site by the combined action of a chemical guidance and the oviduct movement. A working model is proposed to explain how these two mechanisms may operate to transport spermatozoa to the fertilization site, probably as an evolutionary adaptation to maximize the chance of fertilizing an egg.

show abstract

Section: Working Model To Explain Sperm Transport and Retention At Thmentioning

confidence: 99%

Sperm transport and retention at the fertilization site is orchestrated by a chemical guidance and oviduct movement

Guidobaldi

Teves²,

Uñates³

et al. 2012

Reproduction

View full text Add to dashboard Cite

show abstract

“…Spermatozoa are attracted to molecules with diverse chemical nature, such as peptides, odorants, and steroids (Giojalas et al, ). Progesterone, in particular, has been studied extensively with human and rabbit spermatozoa (Blengini et al, ; Gatica et al, ; Guidobaldi, Teves, Uñates, Anastasía & Giojalas, ; Oren‐Benaroya, Orvieto, Gakamsky, Pinchasov, & Eisenbach, ; Teves et al, , , ; Uñates et al, ). This hormone is secreted by the cumulus cells that surround the egg after ovulation (Bar‐Ami, Gitay‐Goren, & Brandes, ; Chian, Ao, Clarke, Tulandi, & Tan, ; Guidobaldi et al, ; Vanderhyden & Tonary, ; Yamashita, Shimada, Okazaki, Maeda, & Terada, ), and may form a chemical gradient from the center toward the periphery of the cumulus layer and beyond (Guidobaldi et al, ; Teves et al, ).…”

Section: Introductionmentioning

confidence: 99%

An intact acrosome is required for the chemotactic response to progesterone in mouse spermatozoa

Guidobaldi

Hirohashi

Cubilla

et al. 2017

Molecular Reproduction Devel

View full text Add to dashboard Cite

Mammalian sperm become fertilization-competent in the oviduct, during a process known as capacitation that involves the acquisition of the ability to exocytose the acrosome but also the chemotactic responses – both of which contribute to successful fertilization. Chemotaxis is used by spermatozoa to orient and to locate the egg; the acrosome reaction facilitates sperm binding to and fusing with the egg membrane. Mammalian spermatozoa are able to sense picomolar concentrations of progesterone, which drives chemotactic behavior. The state of the acrosome during the chemotactic response, however, is unknown. Genetically modified mouse spermatozoa were employed in a chemotaxis assay under fluorescence microscopy to evaluate the acrosome status while swimming, allowing us to elucidate the acrosome integrity of sperm responding to progesterone-induced chemotaxis. We first showed that wild-type mouse spermatozoa chemotactically respond to a gradient of progesterone, and that the genetic modifications employed do not affect the chemotactic behavior of sperm to progesterone. Next, we found that acrosome-intact, but not acrosome-reacted, spermatozoa orient and respond to picomolar concentrations of progesterone and that chemotaxis normally occurs prior to the acrosome reaction. Our results suggest that premature commitment to acrosome exocytosis leads to navigation failure, so proper control and timing of the acrosome reaction is required for fertilization success and male fertility.

show abstract

“…The development of the fertilization‐competent state occurs through an incompletely understood process called “capacitation,” which refers to the physiological changes that render spermatozoa competent to fertilize an egg (Visconti et al, ). Pre‐contact communication by chemotaxis has been demonstrated between gametes in pig, rabbit, human, and mice (Lee et al, ; Cohen‐Dayag et al, ; Sliwa, ; Spehr et al, ; Teves et al, ). The involvement of different molecules in chemokinesis and in the chemotactic attraction of human sperm to the oocyte is a fascinating and evolving concept.…”

mentioning

confidence: 99%

A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis

et al. 2013

View full text Add to dashboard Cite

Although recent evidence indicates that several chemokines and defensins, well-known as inflammatory mediators, are expressed in the male and female reproductive tracts, the location and functional significance of chemokine networks in sperm physiology and sperm reproductive tract interactions are poorly understood. To address this deficiency in our knowledge, we examined the expression and function in sperm of CCR6, a receptor common to several chemoattractant peptides, and screened several reproductive tract fluids for the presence of specific ligands. CCR6 protein is present in mouse and human sperm and mainly localized in the sperm tail with other minor patterns in sperm from mice (neck and acrosomal region) and men (neck and midpiece regions). As expected from the protein immunoblotting and immunofluorescence results, mouse Ccr6 mRNA is expressed in the testis. Furthermore, the Defb29 mRNA encoding the CCR6 ligand, β-defensin DEFB29, is expressed at high levels in the epididymis. As determined by protein chip analysis, several chemokines (including some that act through CCR6, such as CCL20/MIP-3α (formerly Macrophage Inflammatory Protein 3α) and protein hormones were present in human follicular fluid, endometrial secretions, and seminal plasma. In functional chemotaxis assays, capacitated human sperm exhibited a directional movement towards CCL20, and displayed modifications in motility parameters. Our data indicate that chemokine ligand/receptor interactions in the male and female genital tracts promote sperm motility and chemotaxis under non-inflammatory conditions. Therefore, some of the physiological reactions mediated by CCR6 ligands in male reproduction extend beyond a pro-inflammatory response and might find application in clinical reproduction and/or contraception.

show abstract

Progesterone sperm chemoattraction may be modulated by its corticosteroid-binding globulin carrier protein

Cited by 17 publications

References 20 publications

Sperm transport and retention at the fertilization site is orchestrated by a chemical guidance and oviduct movement

Sperm transport and retention at the fertilization site is orchestrated by a chemical guidance and oviduct movement

An intact acrosome is required for the chemotactic response to progesterone in mouse spermatozoa

A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis

Contact Info

Product

Resources

About