2023
DOI: 10.1016/j.anireprosci.2023.107264
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Progesterone-stimulated endometrial cell conditioned media increases in vitro produced bovine embryo blastocyst formation

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Cited by 1 publication
(6 citation statements)
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“…During a recent study, we also detected an increase in IVP bovine blastocyst formation when embryos were cultured in endometrial cell-conditioned media ( Oliver et al 2023 ). The conditioned media were prepared by culturing primary bovine EEp or EF for 12 h in a standard cell culture media (Roswell Park Memorial Institute; RPMI) containing 5% FBS and 15 ng of P4.…”
Section: Discussionmentioning
confidence: 86%
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“…During a recent study, we also detected an increase in IVP bovine blastocyst formation when embryos were cultured in endometrial cell-conditioned media ( Oliver et al 2023 ). The conditioned media were prepared by culturing primary bovine EEp or EF for 12 h in a standard cell culture media (Roswell Park Memorial Institute; RPMI) containing 5% FBS and 15 ng of P4.…”
Section: Discussionmentioning
confidence: 86%
“…EF) were tested for their capacity to improve in vitro cultured embryo development during coculture with some success, mostly related to blastocyst quality ( Kuzan & Wright 1982 , Voelkel et al 1985 ). More recent studies involving bovine EEp have reported increased blastocyst formation when IVP bovine embryos are cocultured with EEp monolayers or cell-conditioned media ( Sponchiado et al 2020 , Oliver et al 2023 ). During a study by Lakshmi Devi et al (2022) , coculture of IVP buffalo (Bubaline) embryos with P4 and estradiol (E2)-stimulated EEp or cell-conditioned media improved blastocyst formation and hatching.…”
Section: Discussionmentioning
confidence: 99%
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