Progesterone stimulates cortisol production in the maturing bovine cumulus-oocyte complex

Anbo, Nobuhiro; Suzuki, Akari; Mukangwa, Memory; Takahashi, Ryota; Muranishi, Yuki; Tetsuka, Masafumi

doi:10.1016/j.theriogenology.2022.06.019

Cited by 4 publications

(2 citation statements)

References 34 publications

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“…In contrast, PGRMC1 does not change across the cycle, and reduced levels of PGRMC2 have been related to endometriosis in macaques (Keator et al, 2012). In addition, P4 levels mediated by progesterone receptors have recently been linked to a beneficial induction of cortisol production in the bovine oocyte during maturation (Anbo et al, 2022), which may encourage further research regarding the interplay between the actions of steroid receptors in reproduction.…”

Section: Discussionmentioning

confidence: 98%

NR3C1 and glucocorticoid-regulatory genes mRNA and protein expression in the endometrium and ampulla during the bovine estrous cycle

Ruiz-Conca

Gardela

Olvera-Maneu

et al. 2022

Research in Veterinary Science

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Section: Discussionmentioning

confidence: 98%

NR3C1 and glucocorticoid-regulatory genes mRNA and protein expression in the endometrium and ampulla during the bovine estrous cycle

Ruiz-Conca

Gardela

Olvera-Maneu

et al. 2022

Research in Veterinary Science

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“…For instance, cortisol may promote luteinization, as glucocorticoids have been shown to increase P4 production in cultured GCs from various species, including cattle [14][15][16][17][18][19][20][21]. P4 may stimulate cortisol production, as we previously found that P4 upregulates HSD11B1 expression and the conversion of cortisone to cortisol in the maturing cumulus oophorus [22]. If this is the case in luteinized GCs (LGCs), P4 may facilitate its own production in a positive feedback loop via cortisol.…”

Section: Introductionmentioning

confidence: 97%

Progesterone modulates HSD11B1-mediated cortisol production in luteinized bovine granulosa cells

Mukangwa

Tetsuka

2023

Journal of Reproduction and Development

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Progesterone (P4) and cortisol production increase in luteinized granulosa cells (LGCs) during the periovulatory period, but their interaction is not well established. Therefore, we investigated their interaction in cultured bovine LGCs. Granulosa cells were collected from follicles of 2-5 mm in diameter and cultured in DMEM/F-12 supplemented with 10% fetal calf serum for up to 14 days. P4 production and the expression of steroidogenic acute regulatory protein (STAR), cholesterol side-chain cleavage enzyme (CYP11A1), and 3β-hydroxysteroid dehydrogenase type 1 (HSD3B1) rapidly increased until day 10 and remained high thereafter. No de novo of cortisol from P4 was detected during the culture period.The expression of 11β-hydroxysteroid dehydrogenase type 1 (HSD11B1), which converts cortisone to cortisol, increased dramatically on day two, decreased until day 8, and remained relatively constant. To investigate how P4 and cortisol influence each other's production, LGCs were treated with trilostane (a P4 synthesis inhibitor), nomegestrol acetate (NA, a synthetic progestogen), P4, and/or cortisol for 24 h on days 6 and 12 of culture. Trilostane suppressed P4 and STAR expression while elevating HSD11B1 and HSD3B1 expression and cortisol production. Concomitant treatment with NA or P4 dose-dependently decreased cortisol production and HSD11B1 and HSD3B1 expression but elevated STAR expression in both days 6 and 12. Conversely, cortisol treatment increased HSD11B1 and HSD3B1 expression and decreased STAR expression without influencing P4 production. These results indicate that progestogens suppress cortisol production by modulating HSD11B1 expression and that progestogens and cortisol differentially regulate STAR, HSD3B1, and HSD11B1 expression in bovine LGCs.

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Local Regulatory Changes of HSD11B1 and NR3C1 in the Follicular and Luteal Tissue During Experimentally Induced Ovulation in the Cow

Berisha,

Pfaffl,

Thaqi

2024

Reprod Domestic Animals

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The objective of the study was to characterise the expression patterns of the two key components of cortisol action namely HSD11B1 (11‐beta‐hydroxysteroid dehydrogenase type 1) and NR3C1 (nuclear receptor subfamily 3, group C, member 1, also known as the glucocorticoid receptor) in superovulation induced bovine follicles during the periovulation and subsequent corpus luteum (CL) formation. Bovine ovaries containing preovulatory follicles or CL were timely defined during induced ovulation as follows: 0 h before GnRH (Gonadotropin‐releasing hormone) application, and 4, 10, 20, 25 (follicles) and 60 h (early CL) after GnRH. The low mRNA expression of HSD11B1 and NR3C1 in the follicle group before the GnRH application increased significantly in the follicle group 20 h after GnRH and remained high afterward also in the early CL group. In contrast, the high NR3C1 mRNA decreased in follicles 25 h after GnRH (close to ovulation) and significantly increased again after ovulation (early CL). Our results indicated the involvement of HSD11B1 and NR3C1 as the two key components of cortisol action in the local mechanisms coordinating final follicle maturation, ovulation, follicular‐luteal transition and CL development in the cow.

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Progesterone stimulates cortisol production in the maturing bovine cumulus-oocyte complex

Cited by 4 publications

References 34 publications

NR3C1 and glucocorticoid-regulatory genes mRNA and protein expression in the endometrium and ampulla during the bovine estrous cycle

NR3C1 and glucocorticoid-regulatory genes mRNA and protein expression in the endometrium and ampulla during the bovine estrous cycle

Progesterone modulates HSD11B1-mediated cortisol production in luteinized bovine granulosa cells

Local Regulatory Changes of HSD11B1 and NR3C1 in the Follicular and Luteal Tissue During Experimentally Induced Ovulation in the Cow

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