Progestins as endocrine disrupters in aquatic ecosystems: Concentrations, effects and risk assessment

Fent, Karl

doi:10.1016/j.envint.2015.06.012

Cited by 203 publications

(141 citation statements)

References 140 publications

(238 reference statements)

Supporting

Mentioning

136

Contrasting

Unclassified

Order By: Relevance

“…However, compared to estrogens, the occurrence, fate and effects of progestins were poorly studied. They are found in effluents and in surface waters (rivers, lakes, streams) and ground waters at concentrations up to tens of ng/L, but also in sediments from rivers at concentrations up to tens of ng/g (for review see (Besse and Garric 2009, Liu et al 2011, Fent 2015. Moreover, progestins are potent developmental and reproductive toxicants for aquatic organisms (for review see Zeilinger et al 2009, Kumar et al 2015.…”

Section: Introductionmentioning

confidence: 99%

“…Among these progestins, levonorgestrel (LNG) is a synthetic progestin structurally related to testosterone (19-Nortestosterone derivative), used alone or in association with an estrogen such as ethinylestradiol for contraception purposes (emergency contraceptives or birth control pills). LNG has been detected in some effluents, sediments, ground water, tap water, but also in surface water of rivers at concentrations up to 38 ng/L (Vulliet et al 2008, Besse and Garric 2009, Al-Odaini et al 2010, Liu et al 2011, Vulliet and Cren-Olivé 2011, Fent 2015. LNG exerts biological activities that differ from the natural progestin (progesterone) since it has progestagenic and androgenic activities (Besse and Garric 2009) and also estrogenic activities both in vitro and in vivo (Jeng et al 1992, Zucchi et al 2012, Creusot et al 2014, Kroupova et al 2014.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Additive effects of levonorgestrel and ethinylestradiol on brain aromatase ( cyp19a1b ) in zebrafish specific in vitro and in vivo bioassays

Hinfray¹,

Tebby

Garoche³

et al. 2016

Toxicology and Applied Pharmacology

View full text Add to dashboard Cite

Estrogens and progestins are widely used in combination in human medicine and both are present in aquatic environment. Despite the joint exposure of aquatic wildlife to estrogens and progestins, very little information is available on their combined effects. In the present study we investigated the effect of ethinylestradiol (EE2) and Levonorgestrel (LNG), alone and in mixtures, on the expression of the brain specific ER-regulated cyp19a1b gene. For that purpose, recently established zebrafish-derived tools were used: (i) an in vitro transient reporter gene assay in a human glial cell line (U251-MG) co-tranfected with zebrafish estrogen receptors (zfERs) and the luciferase gene under the control of the zebrafish cyp19a1b gene promoter and (ii) an in vivo bioassay using a transgenic zebrafish expressing GFP under the control of the zebrafish cyp19a1b gene promoter (cyp19a1b-GFP). Concentrationresponse relationships for single chemicals were modeled and used to design the mixture experiments following a ray design. The results from mixture experiments were analyzed to predict joint effects according to concentration addition and statistical approaches were used to characterize the potential interactions between the components of the mixtures (synergism/antagonism). We confirmed that some progestins could elicit estrogenic effects in fish brain. In mixtures, EE2 and LNG exerted additive estrogenic effects both in vitro and in vivo, suggesting that some environmental progestin could exert effects that will add to those of environmental (xeno-)estrogens. Moreover, our zebrafish specific assays are valuable tools that could be used in risk assessment for both single chemicals and their mixtures.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Additive effects of levonorgestrel and ethinylestradiol on brain aromatase ( cyp19a1b ) in zebrafish specific in vitro and in vivo bioassays

Hinfray¹,

Tebby

Garoche³

et al. 2016

Toxicology and Applied Pharmacology

View full text Add to dashboard Cite

show abstract

“…liquid chromatographic-mass spectrometric method with solid-phase extraction, see later) decreased the limit of detection, resulting in an increasing number of detectable SOCs in surface and ground water, as well [47,48]. Nowadays, their reported presence are in a concentration range from a few ng/L to often tens or hundreds of ng/L (estrogens: 0.20-480.00 ng/L, progestogens: 0.07-22.20 ng/L) in surface waters [47,[49][50][51]. The catchment area of the largest shallow lake of Central Europe is a habitat of several molluscs (e.g.…”

Section: Steroid Type Edcs In the Aquatic Environmentmentioning

confidence: 99%

“…Despite significant use, their ecotoxicological implications are poorly understood in environment. According to Fent, only about 50% of the progestogens in use have been analyzed for their environmental occurrence and effects in aquatic organisms [49].…”

Section: Progestogens As Neuroendocrine Disruptors: An Outlook On Thementioning

confidence: 99%

“…A large number of studies investigating the occurrence and effects of natural and synthetic estrogen hormones (e.g. ethinylestradiol, estradiol, estrone and estriol), and the risk is now well documented [47,49,75,76]. Several studies have also been conducted on the risk related to anti-androgens [77], but contrast to these, surprisingly, relatively few data are published about the occurrence of progestogens in different waters [34,41,49] and mainly their neuroendocrine effects on non-target freshwater organisms, including particularly invertebrates [49].…”

Section: Progestogens As Neuroendocrine Disruptors: An Outlook On Thementioning

confidence: 99%

See 1 more Smart Citation

Pond Snail Reproduction as Model in the Environmental Risk Assessment: Reality and Doubts

Pirger¹,

Zrínyi²,

Maász³

et al. 2018

Biological Resources of Water

View full text Add to dashboard Cite

In European limnetic systems, the most relevant endocrine-disrupting chemicals (EDCs) of steroid type are the natural and synthetic hormones, phytosterols, pesticides, biocides and other chemicals produced by the plastic industry. Their presence in aquatic ecosystems represents a potentially adverse environmental and public health impact. Furthermore, this is a warning signal that the current handling of pharmaceuticals needs to be further improved. Nowadays, it has become clear that EDCs have specific disturbing effects on the neuroendocrine system of invertebrate and vertebrate aquatic animals, particularly gastropods. Among a latter, pond snail (Lymnaea stagnalis) has been used as the first aquatic non-arthropod test organism in studying the effect of EDCs because they are sensitive to various anthropogenic steroids, like progestogens. Investigating a variety of reproductive endpoints of Lymnaea, such as fecundity, oocyte production, egg mass production, the quality of egg masses, the shell size in development and after egg-laying, the time window of cell division in the offspring, the metabolite content of single-cell zygotes and egg albumen has concluded that progestogen contaminations in water are detrimental for reproduction and early stage development of Lymnaea. This chapter is an attempt to show whether Lymnaea reproduction, despite many altering reproductive endpoints, is a suitable model for environmental risk assessment or not.

show abstract

Size‐Dependent Inhibition of Sperm Motility by Copper Particles as a Path toward Male Contraception

Chattopadhyay

Magdanz

Hernández-Meliá

et al. 2022

Advanced NanoBiomed Research

View full text Add to dashboard Cite

show abstract

Progestins as endocrine disrupters in aquatic ecosystems: Concentrations, effects and risk assessment

Cited by 203 publications

References 140 publications

Additive effects of levonorgestrel and ethinylestradiol on brain aromatase ( cyp19a1b ) in zebrafish specific in vitro and in vivo bioassays

Additive effects of levonorgestrel and ethinylestradiol on brain aromatase ( cyp19a1b ) in zebrafish specific in vitro and in vivo bioassays

Pond Snail Reproduction as Model in the Environmental Risk Assessment: Reality and Doubts

Size‐Dependent Inhibition of Sperm Motility by Copper Particles as a Path toward Male Contraception

Contact Info

Product

Resources

About