Proglycogen and macroglycogen: artifacts of glycogen extraction?

James, Anthony P.; Barnes, Phillip D.; Palmer, T.N.; Fournier, Paul A.

doi:10.1016/j.metabol.2007.11.017

Cited by 23 publications

(10 citation statements)

References 36 publications

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“…To determine the mechanisms involved in glycogen accumulation in this model, acid-insoluble and -soluble glycogen fractions were determined. Commonly referred to as pro-and marcoglycogen, these subspecies can be separated by their differential protein-to-carbohydrate ratio (17) and, possibly, their association with cytoskeletal elements (18). In skeletal muscle, these two forms of glycogen have different metabolic sensitivities, with acid-insoluble glycogen being more sensitive to degradation (36).…”

Section: Discussionmentioning

confidence: 99%

Exercise training does not correct abnormal cardiac glycogen accumulation in thedb/dbmouse model of type 2 diabetes

Shearer¹,

Ross

Hughey³

et al. 2011

American Journal of Physiology-Endocrinology and Metabolism

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Substrate imbalance is a well-recognized feature of diabetic cardiomyopathy. Insulin resistance effectively limits carbohydrate oxidation, resulting in abnormal cardiac glycogen accumulation. Aims of the present study were to 1) characterize the role of glycogen-associated proteins involved in excessive glycogen accumulation in type 2 diabetic hearts and 2) determine if exercise training can attenuate abnormal cardiac glycogen accumulation. Control (db(+)) and genetically diabetic (db/db) C57BL/KsJ-lepr(db)/lepr(db) mice were subjected to sedentary or treadmill exercise regimens. Exercise training consisted of high-intensity/short-duration (10 days) and low-intensity/long-duration (6 wk) protocols. Glycogen levels were elevated by 35-50% in db/db hearts. Exercise training further increased (2- to 3-fold) glycogen levels in db/db hearts. Analysis of soluble and insoluble glycogen pools revealed no differential accumulation of one glycogen subspecies. Phosphorylation (Ser(640)) of glycogen synthase, an indicator of enzymatic fractional activity, was greater in db/db mice subjected to sedentary and exercise regimens. Elevated glycogen levels were accompanied by decreased phosphorylation (Thr(172)) of 5'-AMP-activated kinase and phosphorylation (Ser(79)) of its downstream substrate acetyl-CoA carboxylase. Glycogen concentration was not associated with increases in other glycogen-associated proteins, including malin and laforin. Novel observations show that exercise training does not correct diabetes-induced elevations in cardiac glycogen but, rather, precipitates further accumulation.

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Section: Discussionmentioning

confidence: 99%

Exercise training does not correct abnormal cardiac glycogen accumulation in thedb/dbmouse model of type 2 diabetes

Shearer¹,

Ross

Hughey³

et al. 2011

American Journal of Physiology-Endocrinology and Metabolism

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show abstract

“…However, the methodology used to determine the concentrations of PG and MG has been challenged, as it may lead to overestimation of the PG fraction (James et al 2008). Notwithstanding this, the proportion of total glycogen as MG is recorded as 40% in horses (Bröjer et al 2002), 24-25% (estimated) in pigs (Young et al 2009), and 46-57% in sheep .…”

Section: Glycogen Structurementioning

confidence: 99%

Regulation of post-mortem glycolysis in ruminant muscle

Ferguson

Gerrard²

2014

Anim. Prod. Sci.

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Abstract. As a tissue, muscle has the unique ability to switch its metabolic source of ATP, the energy currency underpinning muscle function. During oxygen debt, such as that occurring immediately following the death of animals, anaerobic metabolism is initiated in an attempt to restore homeostasis within the muscle. The cascade of biochemical events that are initiated is paramount in the context of meat quality. This review revisits this reasonably well-known subject but takes a new perspective by drawing on the understanding outside the traditional discipline of meat science. Our understanding of the intrinsic regulators of glycolytic flux has improved but knowledge gaps remain. Further efforts to understand how the glycolytic enzyme kinetics are influenced by both pre-and post-slaughter factors will be beneficial in the ongoing quest to maximise fresh meat quality.

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“…Despite the continuing controversy surrounding the existence of two distinct pools of glycogen (for review see [48,59]), the results from the acid extraction protocol confirmed those obtained from the PAS stain procedure. Endurance Fig.…”

Section: Endurance Training Does Not Reduce Glycogen Content In Slow mentioning

confidence: 60%

Aerobic training as an adjunctive therapy to enzyme replacement in Pompe disease

Nilsson

Samjoo

Hettinga

et al. 2012

Molecular Genetics and Metabolism

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Proglycogen and macroglycogen: artifacts of glycogen extraction?

Cited by 23 publications

References 36 publications

Exercise training does not correct abnormal cardiac glycogen accumulation in thedb/dbmouse model of type 2 diabetes

Exercise training does not correct abnormal cardiac glycogen accumulation in thedb/dbmouse model of type 2 diabetes

Regulation of post-mortem glycolysis in ruminant muscle

Aerobic training as an adjunctive therapy to enzyme replacement in Pompe disease

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