“…Approaches to overcome these limitations include leaf analysis in the early growing season, leaf blade and petiole analysis (in grapevines), and the analysis of flowers, dormant shoots, bark, and xylem sap as alternatives to conventional leaf analysis, as summarized in Table 2. (1) Leaf analysis Analysis of leaf samples collected in the early growing season, particularly at 28 DAFB, can determine the nutrient status of N, P, K, Ca, Mg, Zn, Mn, and B (but not Fe or Cu) in apple trees (Uçgun and Gezgin, 2017) (Table 2). Rubio-Covarrubias et al (2009) examined which of the N forms in 'Fantasia' nectarine leaves are better indicators of the N status of the trees; stable N indicators (total N and chlorophyll SPAD) in the leaves sampled in July over three years could be used to diagnose N deficiency, whereas soluble N compounds (NH 4 -N and NO 3 -N) could be used to diagnose N excess under high N supply, so the combination could be Bark analysis allows the early prognosis of Fe chlorosis in peach trees at least a month earlier than flower analysis Karagiannidis et al (2008) Xylem sap…”