Prognosis associated with cutaneous melanoma metastases

Pan, Yue; Haydon, Andrew; McLean, Catriona; McDonald, Priska B B; Kelly, John W.

doi:10.1111/ajd.12293

Cited by 8 publications

(4 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the early stage, melanoma can be surgically removed, and the 5-year survival rate of melanoma patients is as high as 98% ( 5 ). Nevertheless, melanoma can easily metastasize to regional lymph nodes and result in poor prognosis, with a 5-year survival rate of only 16% ( 6 , 7 ). Various methods are used for the treatment of melanoma, including surgery, chemotherapy, radiation therapy, and immunotherapy ( 8 , 9 ).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-153-3p sensitizes melanoma cells to dacarbazine by suppressing ATG5-mediated autophagy and apoptosis

Hou¹,

Guo²,

Haiying³

et al. 2020

Transl Cancer Res TCR

View full text Add to dashboard Cite

Background: Dacarbazine is one of the most commonly used chemotherapeutic agents for the treatment of melanoma; however, only 5-10% of patients benefit from this treatment. ) has a tumor-suppressive effect in melanoma. In the present study, we found that miR-153-3p was downregulated in melanoma cell lines (A357 and M14). Methods:The target relationship between miR-153-3p and Autophagy-related gene 5 (ATG5) was confirmed by Dual-Luciferase Reporter Assay. Cell Counting Kit-8, flow cytometry, immunofluorescence, and Western blot were used to examine cell viability, apoptosis, and autophagy, respectively.Results: miR-153-3p overexpression decreased the half-maximal inhibitory concentration value of dacarbazine, while increasing the apoptotic rate in both A357 and M14 cells. Moreover, miR-153-3p enhanced dacarbazine-induced autophagy in melanoma cells. Our bioinformatics study revealed that ATG5 is one of the potential targets of miR-153-3p. The overexpression of ATG5 decreased dacarbazine sensitivity and promoted proliferation, as well as inhibited apoptosis and autophagy in melanoma cells. miR-153-3p exhibited suppressive effects via directly binding and downregulating ATG5 expression, which subsequently increased sensitivity to dacarbazine and inhibited proliferation, and enhanced apoptosis and autophagy in melanoma cells. Conclusions:The results of the present study showed that miR-153-3p sensitizes melanoma cells to dacarbazine by suppressing ATG5-mediated autophagy and apoptosis, and provided a basis to explore the functions of miRNAs on drug resistance in the treatment of melanoma.

show abstract

Section: Introductionmentioning

confidence: 99%

MicroRNA-153-3p sensitizes melanoma cells to dacarbazine by suppressing ATG5-mediated autophagy and apoptosis

Hou¹,

Guo²,

Haiying³

et al. 2020

Transl Cancer Res TCR

View full text Add to dashboard Cite

show abstract

“…However, malignant melanoma easily metastasize to regional lymph nodes, and this is associated with a poor prognosis due to a lack of effective interventions. The overall survival of patients thus affected is only 6–9 months, and the 5-year survival rate is only 16% ( 8 , 9 ). Because malignant melanoma that has metastasized is not amenable to conventional radiotherapy and chemotherapy, it is particularly important to identify relvant molecular markers for early diagnosis and treatment.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑150 inhibitors enhance cell apoptosis of melanoma by targeting PDCD4

et al. 2017

View full text Add to dashboard Cite

Malignant melanoma is a tumor with a high mortality rate. Previous studies have demonstrated that the oncogenesis of melanoma is associated with microRNA (miR)-150. However, the role of miR-150 in melanoma and its regulatory mechanisms are still unclear. In the present study, melanoma cancer tissues and adjacent normal tissues were obtained from 20 melanoma patients. The expression level of miR-150 in melanoma tissue and cell lines was detected by reverse transcription-quantitative polymerase chain reaction. miR-150 inhibitors/negative control were transfected into melanoma A375 cells in order to investigate the effects of miR-150 on cell proliferation, apoptosis, cell cycle migration and invasion using a Cell Counting Kit-8, colony formation, Hoechst 33528, flow cytometry, and Transwell assays. The association between miR-150 and programmed cell death protein-4 (PDCD4) was detected by a dual luciferase reporter assay. The functional role of PDCD4 in miR-150-affected melanoma cells was confirmed by small interfering (si)RNA knockdown. Results demonstrated that miR-150 was significantly upregulated and mRNA and protein expressions of PDCD4 were decreased in melanoma cancer tissues as compared with adjacent normal tissues. The level of PDCD4 was inversely associated with the level of miR-150. Transfection of miR-150 inhibitors suppressed cell proliferation, migration, and invasion, while the apoptosis of cells was promoted and G2/M cell arrest was induced. MiR-150 inhibitors enhanced the expression of caspase-8 and p21. The PDCD4 was identified as a direct target gene of miR-150. The effects of miR-150 inhibitors on apoptosis and apoptosis-associated proteins, including caspase-8 and p21, of A375 cells, were reversed following transfection of siRNA-PDCD4. Therefore, miR-150 inhibitors enhance cell apoptosis via upregulation of PDCD4-mediated activation of caspase-8 and p21. These findings demonstrate the potential for a promising therapeutic strategy in the management of melanoma.

show abstract

“… [26] Additionally, distant metastases and thickness of the primary CM were also found to be prominent negative predictors of the survival outcome. [27] After adjustment of multiple confounding factors through IPTW using the propensity score, such as gender, age, tumor histology, primary site and so on, our results showed that the patients undergoing surgical resection had a reduced risk of death compared with those without surgical resection, manifesting a superiority of surgical resection in survival outcome.…”

Section: Discussionmentioning

confidence: 74%

Surgery vs non-surgery in cutaneous melanoma based on SEER database

Liu¹,

Yang

Fu³

et al. 2021

Medicine

View full text Add to dashboard Cite

This study was to assess the survival outcome of cutaneous melanoma (CM) patients with surgery vs non-surgery through inverse probability of treatment weighting (IPTW) using the propensity score. Patients diagnosed as CM were selected from the Surveillance, Epidemiology, and End Results Program (SEER) database. The survival outcome was estimated and compared by IPTW using the propensity score. Totally 2203 CM patients were identified, in which 1921 cases received surgical treatment (surgery group), while 282 cases didn’t (non-surgery group). The median survival time of surgery and non-surgery groups was respectively 150 months and 15 months (unmatched cohort), 70 months and 40 months (matched cohort) and 130 months vs. 75 months (IPTW-weighted cohort). Compared with the non-surgery group, the surgery group had a lower risk of death in unmatched [hazard ratio (HR): 0.647, 95% confidence interval (CI): 0.509–0.821, P < .001] and matched (HR: 0.636, 95%CI: 0.459–0.882, P < .01) cohorts. In multivariate Cox model of IPTW-weighted cohort, the risk of death in the surgery group decreased notably than the non-surgery group (HR: 0.423, 95%CI: 0.383–0.468, P < .001). In conclusion, CM patients receiving surgical treatment are associated with a better survival outcome compared with those without surgical treatment through IPTW using the propensity score.

show abstract

Prognosis associated with cutaneous melanoma metastases

Abstract: We were able to validate the new AJCC melanoma staging system survival for patients with cutaneous metastatic disease. Patients presenting with regional cutaneous metastases have a much better prognosis than those with distant cutaneous metastases.

Cited by 8 publications

References 10 publications

MicroRNA-153-3p sensitizes melanoma cells to dacarbazine by suppressing ATG5-mediated autophagy and apoptosis

MicroRNA-153-3p sensitizes melanoma cells to dacarbazine by suppressing ATG5-mediated autophagy and apoptosis

MicroRNA‑150 inhibitors enhance cell apoptosis of melanoma by targeting PDCD4

Surgery vs non-surgery in cutaneous melanoma based on SEER database

Contact Info

Product

Resources

About