Prognostic and therapeutic significance of the flow cytometric nuclear DNA content in non-small cell lung cancer

Isobe, Hiroshi; Miyata, Hiroshi; Shimizu, Tôru; Haneda, Hitoshi; Hashimoto, Masato; Inoue, Kazuaki; Mizuno, Shigetaka; Kawakami, Yoshikazu

doi:10.1002/1097-0142(19900315)65:6<1391::aid-cncr2820650624>3.0.co;2-k

Cited by 82 publications

(31 citation statements)

References 20 publications

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“…In particular, the independence of P53 expression and cell proliferation, as observed for other tumour types (Silvestrini et al, 1993;Costa et al, 1995), supports the hypothesis that P53 has other biological functions in addition to cell -cycle regulation. Moreover, except for a relation between cell proliferation and histology, such biological variables were independent of pathological characteristics, in agreement with previous data (Kerr et al, 1983;Alama et al, 1990;Isobe et al, 1990;Miyamoto et al, 1991;Silvestrini et al, 1991;Filderman et al, 1992;Quinlan et al, 1992;M0rkve et al, 1993;Scagliotti et al, 1993;Ebina et al, 1994;Passlick et al, 1994).…”

Section: Discussionsupporting

confidence: 91%

“…Several studies have attempted to define the prognostic role of DNA ploidy in patients with all NSCLC histologies and at all stages (Volm et al, 1985;Isobe et al, 1990;Miyamoto et al, 1991;Filderman et al, 1992;M0rkve et al, 1993). Most studies have shown a worse prognosis for patients with aneuploid than for those with diploid tumours (Volm et al, 1985;Isobe et al, 1990;Miyamoto et al, 1991;Filderman et al, 1992), but contrasting results are not lacking (Cibas et al, 1989;M0rkve et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

“…Most studies have shown a worse prognosis for patients with aneuploid than for those with diploid tumours (Volm et al, 1985;Isobe et al, 1990;Miyamoto et al, 1991;Filderman et al, 1992), but contrasting results are not lacking (Cibas et al, 1989;M0rkve et al, 1993). In the present study, DNA abnormalities were observed in a very high percentage of tumours (about 90%), and the prognosis was slightly worse for patients with mutliploid tumours than for those with diploid tumours, even though the difference was not statistically significant.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, it is hoped that, as for some biological factors in other human tumour types (Paradiso et al, 1993;Amadori et al, 1994), predictors of response to different systemic treatments will also be identified in lung cancer. DNA ploidy, cell proliferation, and products of oncogenes or tumour-suppressor genes in lung cancer have been extensively investigated (Volm et al, 1985;Alama et al, 1990;Isobe et al, 1990;Miyamoto et al, 1991;Silvestrini et al, 1991;Tungekar et al, 1991;Filderman et al, 1992;McLaren et al, 1992;Quinlan et al, 1992;M0rkve et al, 1993;Scagliotti et al, 1993;Ebina et al, 1994;Passlick et al, 1994). However, consistent results have not been achieved, probably owing to the heterogeneity in terms of stage and treatment in case series analysed, which implies interference of confounding factors.…”

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confidence: 99%

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P53 expression, DNA ploidy and S-phase cell fraction in operable locally advanced non-small-cell lung cancer

Costa

Silvestrini²,

Mochen³

et al. 1996

Br J Cancer

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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P53 expression, DNA ploidy and S-phase cell fraction in operable locally advanced non-small-cell lung cancer

Costa

Silvestrini²,

Mochen³

et al. 1996

Br J Cancer

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“…A total of 35 studies fulfilled the criteria of selection (Zimmerman et al, 1987;Tirindelli-Danesi et al, 1987;Ten Velde et al, 1988;Volm et al, 1988;Yamaoka et al, 1989;Cibas et al, 1989;Dazzi et al, 1990;Isobe et al, 1990;Sahin et al, 1990;Shiota et al, 1990;Miyamato et al, 1991;Mizumoto et al, 1991;Liewald et al, 1992;Ogawa et al, 1992;Filderman et al, 1992;Morkve et al, 1993;Cheon et al, 1993;Pence et al, 1993;Rice et al, 1993;Ichinose et al, 1993;Lima et al, 1993;Shimizu et al, 1993;Yu et al, 1993;Usuda et al, 1994;Salvati et al, 1994;Tanaka et al, 1995;Pujol et al, 1996;Huang et al, 1996;Nagai et al, 1996;Jeanfaivre et al, 1997;Muguerza et al, 1997;Kolodzeijski et al, 1997;Graziano et al, 1997;Dalquen et al, 1997;Asamura et al, 1999; (Table 1)). Among the 4033 assessable patients, 2626 suffered from NSCLC with aneuploid DNA content (overall frequency of aneuploidy: 0.65; 95% Cl: (0.64-0.67)).…”

Section: Resultsmentioning

confidence: 99%

Aneuploidy and prognosis of non-small-cell lung cancer: a meta-analysis of published data

Choma

Daurès²,

Quantin

et al. 2001

Br J Cancer

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Ploidy status predicts disease-free intervals and short-term survival in numerous human malignancies (Barlogie et al 1983; Friedlander et al 1984). In lung cancer, the prognostic value of ploidy is controversial. The effect of ploidy status on patient outcome has been investigated particularly in non-small-cell lung cancers (NSCLC), a group of different histologies including squamous cell carcinoma (SQC), adenocarcinoma (ADC) and large cell carcinoma (LCC). Although authors from different laboratories have suggested that patients presenting an aneuploid tumour have a shorter survival than patients presenting a nearly diploid tumour, others did not find such a difference. Many factors could explain the differences in estimating the ploidy-survival relationship. Possible interpretation could lie in the heterogeneity of the abnormal DNA patterns gathered under the common term of aneuploidy, i.e. hyperdiploidy, hypodiploidy and multiploidy . Differences in methods used to analyse DNA content could also have been responsible for the above-mentioned controversy inasmuch as some studies were founded on flow cytometry data whereas others used static cytometry. Finally, the difference might be related to the histological and clinical characteristics of the studied patient population.We therefore made a meta-analysis of published studies aimed at determining the prognostic effect of aneuploidy in surgically resected NSCLC. TRIALS AND METHODS Eligibility criteriaThe most conventional definition of ploidy status is as follows: ploidy was determined for each specimen using DNA index which represents the ratio of the cell DNA content of tumour G 0/1 cells to the diploid G 0/1 peak (2n). A coefficient of variation (CV) for this DNA index (DI) has been specifically defined for each study. Thus, a DNA index = 1 defined a near diploid specimen i.e. only one peak of G 0/1 cells in the near diploid region (2n, DNA index = 1) with few G 2 M tumour cells in the tetraploid region (4n Summary In lung cancer, DNA content abnormalities have been described as a heterogeneous spectrum of impaired tumour cell DNA histogram patterns. They are merged into the common term of aneuploidy and probably reflect a high genotypic instability. In non-small-cell lung cancer, the negative effect of aneuploidy has been a subject of controversy inasmuch as studies aimed at determining the survival-DNA content relationship have reported conflicting results. We made a meta-analysis of published studies aimed at determining the prognostic effect of aneuploidy in surgically resected non-small-cell lung cancer. 35 trials have been identified in the literature. A comprehensive collection of data has been constructed taking into account the following parameters: quality of specimen, DNA content assessment method, aneuploidy definition, histology and stage grouping, quality of surgical resection and demographic characteristics of the analysed population. Among the 4033 assessable patients, 2626 suffered from non-small-cell lung cancer with aneuploid DNA content ...

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The prognostic significance of fluorodeoxyglucose positron emission tomography imaging for patients with nonsmall cell lung carcinoma

Ahuja

Coleman

Herndon

et al. 1998

Cancer

295

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BACKGROUND A fundamental property of malignant tumors is increased glucose metabolism, which can be estimated by imaging the glucose analog fluorodeoxyglucose (FDG). The aim of this study was to determine whether FDG uptake in lung carcinoma, as measured on positron emission tomography (PET) imaging in patients with newly diagnosed bronchogenic carcinoma, has prognostic significance. METHODS A retrospective review of all patients with a new diagnosis of nonsmall cell lung carcinoma and FDG‐PET imaging was performed. Stage at presentation, cell type, tumor size, and survival data were recorded. For each patient, a standardized uptake ratio (SUR) was calculated for the primary lesion on PET and was correlated with clinical information to determine prognostic significance. RESULTS One hundred fifty‐five patients, with a mean age of 56 years, were enrolled. One hundred eighteen patients (76%) had lesions with SUR values <10 and had a median survival of 24.6 months (95% confidence interval [CI]: 20.9‐41.1). Thirty‐seven patients (24%) had tumor lesions with SUR values >10 and had a median survival of 11.4 months (95% CI: 9.3‐19.4). An SUR >10 correlated with poorer survival (P = 0.0049). Patients with primary lesions >3 cm and an SUR >10 had the worst prognosis, with a median survival of 5.7 months (95% CI: 4.0‐13.1). Multivariate analysis demonstrated than an SUR >10 provided prognostic information independent of the clinical stage and lesion size. CONCLUSIONS On PET studies of patients with lung carcinoma, FDG uptake within the primary lesion correlates with survival. Cancer 1998;83:918‐924. © 1998 American Cancer Society.

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Prognostic and therapeutic significance of the flow cytometric nuclear DNA content in non-small cell lung cancer

Cited by 82 publications

References 20 publications

P53 expression, DNA ploidy and S-phase cell fraction in operable locally advanced non-small-cell lung cancer

P53 expression, DNA ploidy and S-phase cell fraction in operable locally advanced non-small-cell lung cancer

Aneuploidy and prognosis of non-small-cell lung cancer: a meta-analysis of published data

The prognostic significance of fluorodeoxyglucose positron emission tomography imaging for patients with nonsmall cell lung carcinoma

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