Prognostic Biomarker KIF18A and Its Correlations With Immune Infiltrates and Mitosis in Glioma

Abstract

Background: Glioma is globally recognised as one of the most frequently occurring primary malignant brain tumours, making the identification of glioma biomarkers critically significant. The protein KIF18A (Kinesin Family Member 18A) is a member of the kinesin superfamily of microtubule-associated molecular motors and has been shown to participate in cell cycle and mitotic metaphase and anaphase. This is the first investigation into the expression of KIF18A and its prognostic value, potential biological functions, and effects on the immune system and mitosis in glioma patients.Methods: Gene expression and clinicopathological analysis, enrichment analysis, and immune infiltration analysis were based on data obtained from The Cancer Genome Atlas (TCGA), with additional bioinformatics analyses performed. Statistical analysis was conducted in R software. Clinical samples were used to evaluate the expression of KIF18A via immunohistochemical staining. In addition, the expression level of KIF18A was validated on U87 cell line.Results: Our results highlighted that KIF18A plays a key role as an independent prognostic factor in patients with glioma. KIF18A was highly expressed in glioma tissues, and KIF18A expression was associated with age, World Health Organization grade, isocitrate dehydrogenase (IDH) status, 1p/19q codeletion, primary therapy outcome, and overall survival (OS). Enrichment analysis revealed that KIF18A is closely correlated with the cell cycle and mitosis. Single sample gene set enrichment analysis (ssGSEA) analysis revealed that KIF18A expression was related to the immune microenvironment. The increased expression of KIF18A in glioma was verified in clinical samples and U87 cell line.Conclusion: The identification of KIF18A as a new biomarker for glioma could help elucidate how changes in the glioma cell and immune microenvironment promote glioma malignancy. With further analysis, KIF18A may serve as an independent prognostic indicator for human glioma.

Dynamic Regulation Genes at Microtubule Plus Ends: A Novel Class of Glioma Biomarkers

“…KIF18A is an MT depolymerizing kinesin that ensures chromosome stability during mitosis by inhibiting MT dynamics without disrupting their stability [ 51 ]. KIF18A upregulation contributes to the malignant progression and unfavorable prognosis of various types of cancer, including breast, hepatocellular, clear cell renal, prostate, esophageal carcinomas, and lung adenocarcinoma, especially glioma [ 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 ]. SLAIN2 promotes the nucleation and elongation of cytoplasmic MT and suppresses their catastrophes, which is necessary for maintaining the normal structure of the cytoskeleton [ 62 ].…”

The comprehensive analysis of m6A-associated anoikis genes in low-grade gliomas

“…The expression level of KIF18A in LGG tissues was higher, and which was positively correlated with the size of the tumor and the proliferation ability of the tumor cells [24]. Studies have also found that high expression of KIF18A may indicate an increased risk of malignant transformation from low-grade gliomas to high-grade gliomas [24,25]. BRCA1 is a genetic gene associated with the breast cancer, which is involved in biological processes such as DNA repair and gene expression regulation.…”

“…BRCA1 is a genetic gene associated with the breast cancer, which is involved in biological processes such as DNA repair and gene expression regulation. There is no de nite study of BRCA1 in glioma, but it has been reported that the mutation of BRCA1 gene may be related to some types of brain tumor, such as metastatic brain tumor of breast cancer and ependymoma [25]. BRCA1 gene methylation may also play a crucial role in tumor development [26].…”

The Comprehensive Analysis of m6A-Associated Anoikis Genes in Low-Grade Gliomas

“…The expression level of KIF18A in LGG tissues was higher, which was positively correlated with the size of the tumor and the proliferation ability of the tumor cells [ 28 ]. Studies have also found that high expression of KIF18A may indicate an increased risk of malignant transformation from low-grade gliomas to high-grade gliomas [ 28 , 29 ]. BRCA1 is a gene associated with breast cancer that is involved in biological processes such as DNA repair and gene expression regulation.…”