Prognostic estimation of survival of colorectal cancer patients with the quantitative histochemical assay of G6PDH activity and the multiparameter classification program CLASSIF1

Abstract

Prognosis of colorectal cancer patients that show similar histopathology may vary substantially. An attempt was made to improve prognosis by the self‐learning classification program CLASSIF1, based on automated multiparameter analysis of quantitative histochemical and clinical parameters of 64 colorectal carcinomas and adjacent normal mucosae. The histochemical parameters applied were the oxygen‐insensitivity assay of glucose‐6‐phosphate dehydrogenase (G6PDH) activity, a valid discriminator between normal and cancerous mucosae, and related parameters CuZn‐ and Mn‐superoxide dismutase (SOD) levels, and lipid peroxidation (LPO) capacity. Data were processed on the basis of a postoperative follow‐up of minimally 32 and maximally 56 months. CLASSIF1 selected the parameters oxygen insensitivity of G6PDH activity, CuZn‐SOD and Mn‐SOD levels, LPO capacity, lymph node metastasis, Dukes' stage, and age for the highest prognostic value. On the basis of these selected parameters, CLASSIF1 correctly predicted favorable outcome in 100% of the surviving patients and fatal outcome in 64% of the deceased patients. G6PDH activity appeared to be the major information carrier for CLASSIF1. On the basis of G6PDH activity parameters alone, 96% of the surviving patients and 55% of the deceased patients were correctly classified. In comparison, estimation of prognosis on the basis of Dukes' stage alone resulted in 71% correctly classified surviving patients and 61% of patients who died. It is concluded that the self‐learning classification program CLASSIF1, on the basis of quantitative histochemical and clinical parameters, is the best prognostic estimator for colon cancer patients yet available. Cytometry (Comm. Clin. Cytometry) 38:176–183, 1999. © 1999 Wiley‐Liss, Inc.

Oxygen Insensitivity of the Histochemical Assay of Glucose-6-phosphate Dehydrogenase Activity for the Detection of (Pre)Neoplasm in Rat Liver

“…A value of 60% for the RA in (pre)neoplastic foci is in agreement with values found in adenocarcinomas of colon and pancreatic carcinoma in humans (Van Driel et al 1997,1999a. These large differences in formazan production between normal liver tissue and (pre)neoplastic cells make a discrimination very easy, even when only a few altered cells are present in a biopsy.…”

Anti-metastatic gene therapy utilizing subcutaneous inoculation of EC-SOD gene transduced autologous fibroblast suppressed lung metastasis of Meth-A cells and 3LL cells in mice

“…The tissue was then cut on a cryostat, rinsed in phosphatebuffered saline (PBS) three times, incubated at 37°C overnight in X-gal/Fe solution (5 mm K 3 Fe(CN) 6 , 5 mm K 4 Fe(CN) 6 -3H 2 O, 0.1 m MgCl 2 , 0.2% X-gal (5-bromo-4-chloro-3-indolyl-␤-d-galactosyranosid]), and then examined under a light microscope. 27 In vivo tumor growth Tumor cells (1.0 × 10 6 /0.1 ml of RPMI 1640) were inoculated into the right abdominal flank.…”

“…3 Recently, oxygen radicals and their scavenging enzymes have been implicated in the initiation, progression including metastasis and even the prognosis of cancer. [4][5][6] Oxygen radicals are produced not only by inflammatory cells such as activated neutrophil and macrophage infiltrating tumor foci, but also tumor cells. 7 We and others have reported that oxygen radicals enhance metastasis in various organs such as lung, 8 liver 9 and bone.…”

miR-1 inhibits progression of high-risk papillomavirus-associated human cervical cancer by targeting G6PD

“…Functionally, G6PD supplies ribose and NADPH that support biosynthesis and antioxidant defense, and is thus involved in the oxidative stress response [5]. G6PD levels are elevated in various tumors, including melanoma [6], leukemia [7], colon cancers [8], breast cancers [9], and endometrial carcinomas [10]. Our previous data indicated that elevated G6PD levels were also positively correlated with cervical carcinogenesis in 30 to 40-year-old women infected with HR-HPV-16/18.…”