Prognostic evaluation of cutaneous malignant melanoma: A clinicopathologic and immunohistochemical study

Melanoma Prognosis: A REMARK-Based Systematic Review and Bioinformatic Analysis of Immunohistochemical and Gene Microarray Studies

“…1), the evidence sum has been classified as concordant. In all of these cases except 1 [PCNA (31)], the nature of that agreement was that no significant relationship with expression and clinical outcome was observed. For PCNA, increased gene and protein expression was associated with worse outcome.…”

“…In addition, there were 4 molecules for which disparate findings were observed between different measures of clinical outcome. For GP100 (31,32,37) and cyclin A (32,36,39), an effect was present for disease-free survival but not ACM, whereas for SPP1/osteopontin (45,46) and tPA (43), the effect was observed for MSM or ACM but not disease-free survival.…”

“…Just 2 molecules [PCNA (31) and survivin (18,32)] were significantly associated with clinical outcome at both the gene and protein level. In contrast, 6 molecules were assessed as having no significant relationship with outcome at the protein level but appeared on the Winnepenninckx and colleagues (30) differently expressed gene list [CDK1 (32), cyclin B (32), GRP78 (26), nm23 (33,34), survivin (18,32), and TOPII (32)].…”

“…For PCNA, increased gene and protein expression was associated with worse outcome. In 3 cases (BMI1 (20,32), KI67 (21,31,32,35,36), and gp100 (31,32,37), the results of independent studies were discordant, i.e., the results of IHC-based analysis have disagreed either with respect to the significance of an effect (BMI1 and KI67) or its significance and direction (gp100). In each of those cases, no outcome-related expression change was observed at the gene transcript level in the eligible gene expression dataset (30).…”

Prognosticators of melanoma, the melanoma report, and the sentinel lymph node

“…67 The predictive value of Ki-67 correlated more closely in one study of uveal melanoma with S phase, shorter survival, histological type, and tumor size than did PCNA expression. 68 Others found both Ki-67 and PCNA markers to correlate with survivorship in a study of 93 consecutive melanomas; 69 there was correlation of metastatic behavior to PCNA expression in a study of 143 melanomas in which Ki-67 expression was not assessed. 70 One study of level III and IV melanomas found no correlation of survivorship with PCNA or Ki-67 expression; however, in that study, flow cytometric analysis showing an aneuploid DNA content was associated with a higher risk of metastatic progression.…”