Prognostic factors and Doxorubicin involved in malignant progression of meningioma

Huo, Xu-Lei; Song, Lairong; Wang, Ke; Wang, Hongyi; Li, Da; Li, Huan; Wang, Wei; Wang, Yali; Chen, Lei; Zhao, Zongmao; Wang, Liang; Wu, Zhen

doi:10.1038/s41598-023-28996-0

Cited by 1 publication

(2 citation statements)

References 66 publications

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“…Finally, the cells that had penetrated the lower chamber were treated with formaldehyde and stained using .1% crystal violet dye. Total cells were counted and photographed in five areas, usually seen under a 200× magnification 27 …”

Section: Methodsmentioning

confidence: 99%

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Unravelling the role of immune cells and FN1 in the recurrence and therapeutic process of skull base chordoma

Huo,

Ma,

Wang

et al. 2023

Clinical & Translational Med

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BackgroundSkull base chordoma is a rare and aggressive tumour of the bone that has a high likelihood of recurrence. The fundamental differences in single cells between primary and recurrent lesions remain poorly understood, impeding development of effective treatment approaches.MethodsTo obtain an understanding of the differences in single cells between primary and recurrent chordomas, we performed single‐cell RNA sequencing and T‐cell/B‐cell receptor (BCR) sequencing. This allowed us to delineate the differences between the two types of tumour cells, tumour‐infiltrating lymphocytes, myeloid cells, fibroblasts and B cells. Copy number variants (CNVs) were detected and compared between the tumour types to assess heterogeneity. Selected samples were subjected to immunohistochemistry to validate protein expression. Fluorescence in situ hybridisation experiments, Transwell assays and xenograft mouse models helped verify the role of fibronectin 1 (FN1) in chordoma.ResultsPromoting natural killer (NK) cell and CD8_GZMK T‐cell function or inhibiting the transformation of CD8_GZMK T cells to CD8_ZNF683 T cells and promoting the transformation of natural killer T (NKT) cells to NK cells are promising strategies for preventing chordoma recurrence. Additionally, inhibiting the M2‐like activity of tumour‐associated macrophages (TAMs) could be an effective approach. Antigen‐presenting cancer‐associated fibroblasts (apCAFs) and dendritic cells (DCs) with high enrichment of the antigen‐presenting signature were enriched in primary chordomas. There were fewer plasma cells and BCR clonotypes in recurrent chordomas. Remarkably, FN1 was upregulated, had more CNVs, and was more highly secreted by tumours, macrophages, CD4 T cells, CD8 T cells and fibroblasts in recurrent chordoma than in primary chordoma. Finally, FN1 enhanced the invasion and proliferation of chordomas in vivo and in vitro.ConclusionOur comprehensive picture of the microenvironment of primary and recurrent chordomas provides deep insights into the mechanisms of chordoma recurrence. FN1 is an important target for chordoma therapy.

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Section: Methodsmentioning

confidence: 99%

“…Total cells were counted and photographed in five areas, usually seen under a 200× magnification. 27 …”

Section: Methodsmentioning

confidence: 99%

Unravelling the role of immune cells and FN1 in the recurrence and therapeutic process of skull base chordoma

Huo,

Ma,

Wang

et al. 2023

Clinical & Translational Med

Self Cite

View full text Add to dashboard Cite

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Prognostic factors and Doxorubicin involved in malignant progression of meningioma

Cited by 1 publication

References 66 publications

Unravelling the role of immune cells and FN1 in the recurrence and therapeutic process of skull base chordoma

Unravelling the role of immune cells and FN1 in the recurrence and therapeutic process of skull base chordoma

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