Prognostic impact of DNA ploidy pattern, S‐phase fraction (SPF), and proliferating cell nuclear antigen (PCNA) in patients with primary gastric lymphoma

Belessi, Chrysoula; Parasi, Aikaterini; Manioudaki, Helen S.; Laoutaris, Nikolaos; Legakis, N. C.; Peros, Georgios; Androulakis, Georgios A.

doi:10.1002/jso.10227

Cited by 14 publications

(12 citation statements)

References 38 publications

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“…Their results confirmed that flow cytometric DNA ploidy status was a significant and independent prognostic factor in patients with colorectal carcinoma. However, in multivariate analysis, SPF was the most significant prognostic factor, whereas DNA ploidy was not found to be an independent predictor of survival in bladder carcinoma and primary gastric lymphomas (Belessi et al, 2003;Falkman et al, 2004). In our study, no correlation was found between DNA ploid status, SPF, and clinicopathologic parameters; the correlation of DNA diploidy and SPF with clinical prognosis in colorectal carcinoma needs further study.…”

Section: Discussioncontrasting

confidence: 81%

Pancreatitis‐Associated Protein Is Related Closely to Neoplastic Proliferative Activity in Patients with Colorectal Carcinoma

Cao

Zhang

et al. 2008

The Anatomical Record

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Pancreatitis-associated protein (PAP) is a secretory protein that is not only expressed during acute pancreatitis but also in pancreatic adenocarcinoma, gastric carcinoma, hepatocellular carcinoma, and colorectal carcinoma. Expression in carcinoma might be another characteristic of PAP. The aim of our study was to assess, in 27 patients undergoing surgery for colorectal carcinoma, the expression of the PAP mRNA and to evaluate its association with DNA ploidy and proliferative activity (S-phase fraction, SPF) by reverse transcriptase-polymerase chain reaction (RT-PCR) and flow cytometric analysis (FCM). PAP mRNA was expressed in 29.6% (8 of 27) of the patients with colorectal carcinoma. DNA aneuploid and high SPF were found in 87.5% (7 of 8) of patients with PAP mRNA positive colorectal carcinoma. The serum PAP level was significantly elevated in patients with colorectal carcinoma when compared with the healthy subjects. Twelve of the 27 patients with colorectal carcinoma had high serum PAP concentrations (>25 ng/mL) and the mean SPF was 17.82% 6 8.02%, which was significantly higher compared with the normal colorectal tissue group (7.33% 6 3.18%, P < 0.05). The mean serum PAP concentration of DNA aneuploidy colorectal carcinomas was 46.67 6 17.58 ng/mL, which was significantly different when compared with the DNA diploidy group (19.18 6 8.89 ng/mL, P < 0.05). PAP mRNA expression and serum PAP levels are closely related to neoplastic proliferative activity in patients with colorectal carcinoma. No significant differences are observed between PAP mRNA expression and clinicopathologic parameters (P > 0.05). Anat Rec, 292:249-253, 2009. 2008 Key words: pancreatitis-associated protein; neoplastic proliferative activity; colorectal carcinomaPancreatitis-associated protein (PAP), a member of the PAP/regenerating gene (Reg) family, is a secretory protein that is not only highly expressed during acute pancreatitis but is also overexpressed in pancreatic adenocarcinoma (Lasserre et al., 1992;Zhang et al., 2004;Gironella et al., 2007). Many studies have shown that PAP is expressed in gastric carcinoma, pancreatic cancer, hepatocellular carcinoma, and colorectal carcinoma.

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Section: Discussioncontrasting

confidence: 81%

Pancreatitis‐Associated Protein Is Related Closely to Neoplastic Proliferative Activity in Patients with Colorectal Carcinoma

Cao

Zhang

et al. 2008

The Anatomical Record

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“…Our data are consistent with those published by Aoyagi et al [25], who observed a significant correlation of PCNA PI with depth of invasion, histological differentiation and the clinical stadium of the disease, thus stating that PCNA index is a good prognostic factor in gastric cancers. Many investigators have found Ki-67 and PCNA to be prognostic factors in tumours of the stomach [13][14][15]22,23,[26][27][28][29].…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical evaluation of Ki-67, PCNA and MCM2 proteins proliferation index (PI) in advanced gastric cancer.

Czyżewska

Guzińska‐Ustymowicz²,

Pryczynicz³

et al. 2009

Folia Histochem Cytobiol.

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Abstract:The current study objective was to assess the proliferation indices (PI) of Ki-67, PCNA and MCM2 proteins in advanced gastric cancer and in metastatic lymph node in correlation with certain clinicopathological features and with postoperative survival of patients. The study was conducted in a group of 100 patients with advanced gastric cancers. Involvement of local lymph nodes was present in 36 cases. Immunohistochemical investigations were carried out using monoclonal antibodies against Ki-67 (DAKO), PCNA (DAKO) and polyclonal antibody to MCM2 (Santa Cruz Biotechnology). Visualization of the antigen/antibody complex was performed using LSAB technique (biotin-streptavidin-peroxidase) followed by application of chromogene DAB (DAKO). Statistical analysis revealed no correlations of Ki-67, PCNA and MCM2 PI in tumour tissue or metastatic lymph node with patients' age and gender, tumour location, histological grade, macroscopic type according to Bormann's classification and histological grading by Lauren's and Goseki's classifications. Moreover, no correlation was observed of Ki-67 and MCM2 PI in tumour tissue with histological grading. No correlation was also noted between the proliferation indices of all the three proteins in the affected lymph node and grade of histological differentiation. Such clinicopathological parameters as patients' age and gender, histological grading by Lauren's and Goseki's classifications and lymph node involvement did not correlate with survival time of patients. Furthermore, no statistically significant correlation was shown of postoperative survival time with Ki-67 and MCM2 PI in tumour tissue and metastatic lymph nodes and with PCNA PI in the affected lymph nodes. However, a statistically significant correlation was found of Ki-67, PCNA and MCM2 PI in tumour tissue and metastatic lymph nodes with depth of wall invasion and local lymph node involvement. A statistically significant correlation was also noted between PCNA PI in the main mass of tumour and histological grading. The postoperative survival time of patients exhibited a statistically significant correlation with tumour location and macroscopic type according to Bormann's classification. Correlations on statistical borderline were noted between survival time and depth of gastric wall invasion and PCNA PI in the main mass of tumour.

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“…In some of them the only finding is S-phase prognostic value only in univariate but not in the multivariate analysis (11,20). Joensuu et al (18) found aneuploidy as a negative prognostic value but only in univariate analysis.…”

Section: Discussionmentioning

confidence: 99%

“…Among these factors are the clinical stage, high-grade histologic type, serosal involvement, or proliferating cell nuclear antigen (PCNA) expression (4)(5)(6)(7)(8)(9)(10)(11). Flow cytometry (FC) is a quantitative method of DNA content assessment.…”

Section: Introductionmentioning

confidence: 99%

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Prognostic value of flow cytometry in surgically treated primary gastric lymphoma

Fernández

Rodríguez‐Sanjuán

Mayorga

et al. 2006

Rev. esp. enferm. dig.

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Aim: to investigate whether flow cytometry could help to define the optimal therapeutic strategy of primary gastric lymphomas.Material and method: retrospective study of 46 patients having primary gastric lymphoma -according to Dawson criteria-in Ann Arbor stage I E and II E , who were surgically treated. From selected paraffin-embedded tissue blocks of the tumor, DNA content was studied by flow cytometry (FC). Other pathological tumor features were analysed by hematoxiline-eosine and Giemsa stains as well as immunohistochemical study; any possible influence on postoperative survival was investigated through statistical analysis.Results: the DNA ploidy pattern was diploid in 40 cases (87%) and aneuploid (hyperdiploid) in 6 (13%). Postoperative survival probability (PSP) was 62.7% at 5 years. Statistical analysis showed significant prognostic value for Ann Arbor classification -with higher PSP for stage I E (p = 0.009)-and FC parameters: diploid tumors had higher PSP than aneuploid tumors. Also tumors having S-phase (p = 0.044) or G2-M phase values (p = 0.023) under the respective mean values had higher PSP. No influence on PSP was found for wall invasion, Helicobacter pylori infection, Isaacson's histologic type or resection margin involvement. No significant relationship was appreciated between Isaacson's histologic type and DNA ploidy patterns.Conclusion: FC could be useful in assessing gastric lymphoma prognosis.

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Prognostic impact of DNA ploidy pattern, S‐phase fraction (SPF), and proliferating cell nuclear antigen (PCNA) in patients with primary gastric lymphoma

Abstract: These findings indicate that SPF and PCNA are significant prognostic factors in patients with primary gastric lymphomas. However, in the present study, DNA ploidy had no impact on survival in patients with primary gastric lymphomas.

Cited by 14 publications

References 38 publications

Pancreatitis‐Associated Protein Is Related Closely to Neoplastic Proliferative Activity in Patients with Colorectal Carcinoma

Pancreatitis‐Associated Protein Is Related Closely to Neoplastic Proliferative Activity in Patients with Colorectal Carcinoma

Immunohistochemical evaluation of Ki-67, PCNA and MCM2 proteins proliferation index (PI) in advanced gastric cancer.

Prognostic value of flow cytometry in surgically treated primary gastric lymphoma

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