2010
DOI: 10.1186/1479-5876-8-23
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Prognostic impact of ZAP-70 expression in chronic lymphocytic leukemia: mean fluorescence intensity T/B ratio versus percentage of positive cells

Abstract: BackgroundZAP-70 is an independent negative prognostic marker in chronic lymphocytic leukemia (CLL). Usually, its expression is investigated by flow cytometric protocols in which the percentage of ZAP-70 positive CLL cells is determined in respect to isotypic control (ISO-method) or residual ZAP-70 positive T cells (T-method). These methods, however, beside suffering of an inherent subjectivity in their application, may give discordant results in some cases. The aim of this study was to assess the prognostic s… Show more

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Cited by 20 publications
(24 citation statements)
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“…In a large international standardization project, the best parameter for reporting the results of ZAP-70 measurement was indeed MFI ratio of T-cells/CLL cells [11]. These results have been confirmed by Rossi et al and Kern et al [14,18]. Our results are in concordance with these studies including the prognostic influence on survival parameters.…”
Section: Discussionsupporting
confidence: 83%
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“…In a large international standardization project, the best parameter for reporting the results of ZAP-70 measurement was indeed MFI ratio of T-cells/CLL cells [11]. These results have been confirmed by Rossi et al and Kern et al [14,18]. Our results are in concordance with these studies including the prognostic influence on survival parameters.…”
Section: Discussionsupporting
confidence: 83%
“…The use of other two methods did not result in significant differences in TTT or OS. Measurement of values of ZAP-70 change by mean fluorescence intensity (MFI) ratio has been recently reported as being more reliable, less subjective and therefore superior over assessment of percentage of ZAP-70 positive cells [11,13,14,18]. Measurement using MFI ration reduces the subjective error potentially present when assessing ZAP-70 expression by percentage of positive cells.…”
Section: Discussionmentioning
confidence: 99%
“…1). Isoclonic ratios and T/B ratios were calculated as described (12,(15)(16)(17)(18)(19)29 and see Materials and Methods).…”
Section: Resultsmentioning
confidence: 99%
“…The main problem was to find the correct internal control. Among proposed methods, calculating the ZAP70 T/B ratio is one of the earliest and most common approaches (12,(15)(16)(17)(18). Other methods based on ratios calculated on normal B-cell MFIs have drawbacks such as the very low percentage of residual B-cells or the difficulty of separating them from CLL cells by flow cytometry in the case of weak CD5 expression (11,13,32).…”
Section: T/b Ratio Does Not Reflect Zap70 Expression Levelsmentioning
confidence: 99%
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