Prognostic Implications of DNA Repair, Ploidy and Telomerase in the Malignant Transformation Risk Assessment of Leukoplakia

Thomas, Gigi; S, Tr; George, Saira; Somanathan, Thara; Sarojam, Santhi; Krishnankutti, N; Subramanian, Hariharan; Ankathil, Ravindran

doi:10.31557/apjcp.2020.21.2.309

Cited by 4 publications

(5 citation statements)

References 16 publications

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“…A later prospective observational study, including 181 OL with a variable presence of OED, found the DNA ploidy status to be a reliable marker for progression only in a univariate logistic regression assessment (OR 6.5 with 95% CI 1.9–22.8); this accords with our own univariate analysis. However, the authors did not mention any related assessment for the presence of OED 52 . This should not be omitted, given the recognized association between OED and a DNA aneuploid status 22,53–55 already reported in our previous studies 29,56 .…”

Section: Discussionmentioning

confidence: 92%

“…However, the authors did not mention any related assessment for the presence of OED. 52 This should not be omitted, given the recognized association between OED and a DNA aneuploid status 22,[53][54][55] already reported in our previous studies. 29,56 The highest predictive values for MT have been obtained by combinations of DNA aneuploidy and severe OED.…”

Section: Dna Ploidy Status In Olmentioning

confidence: 88%

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Predictors of malignant transformation in oral leukoplakia and proliferative verrucous leukoplakia: An observational prospective study including the DNA ploidy status

et al. 2023

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BackgroundThis prospective observational study investigated the determinants of malignant transformation (MT) in localized oral leukoplakia (OL) and proliferative verrucous leukoplakia (PVL).MethodsDemographic, clinical, histological, and DNA ploidy status data were collected at enrolment. Survival analysis was performed (MT being the event of interest).ResultsOne‐hundred and thirty‐three patients with OL and 20 patients with PVL entered the study over 6 years (mean follow‐up 7.8 years). The presence of OED, DNA ploidy, clinical presentation, and lesion site were associated with MT in patients with OL in a univariate analysis. In a multivariate model, OED was the strongest predictor of MT in patients with OL. Adding DNA ploidy increased the model's predictive power. None of the assessed predictors was associated with MT in patients with PVL.ConclusionsDNA ploidy might identify a subset OL with low risk or minimal risk of MT, but it does not seem to be a reliable predictor in patients with PVL.

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Section: Discussionmentioning

confidence: 92%

Section: Dna Ploidy Status In Olmentioning

confidence: 88%

Predictors of malignant transformation in oral leukoplakia and proliferative verrucous leukoplakia: An observational prospective study including the DNA ploidy status

et al. 2023

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“…Additionally, abnormal DNA amounts can be detected by the flow cytometric analysis. Therefore, this method could be applied to the study of oral cancer development and the effects of drugs strategies to prevent or decrease the risk of malignant transformation [43,44].…”

Section: Discussionmentioning

confidence: 99%

Genistein anticancer efficacy during induced oral squamous cell carcinoma: an experimental study

Hussein

Attaai

Zahran

2022

J Egypt Natl Canc Inst

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Background About 7 million people die from various types of cancer every year representing nearly 12.5% of deaths worldwide. This fact raises the demand to develop new, effective anticancer, onco-suppressive, and chemoprotective agents for the future fighting of cancers. Genistein exhibits pleiotropic functions in cancer, metabolism, and inflammation. It functions as an antineoplastic agent through its effect on the cell cycle, apoptotic processes, angiogenesis, invasion, and metastasis. Aim of the study The current study aimed to study the genistein onco-suppressive effects during 7,12-dimethylbenz[a]anthracene (DMBA)-induced oral carcinogenesis in hamsters’ buccal pouch utilizing flow cytometry analysis (FMA), as a fast-diagnosing tool, in addition to the histopathology. Material and methods The buccal mucosa of adult male Syrian hamsters was painted with paraffin oil only (group 1), DMBA mixed in mineral oil (group 2), or orally administrated genistein along with painting DMBA (group 2B). The buccal mucosa was utilized for flow cytometric analysis and histopathological examination. Results Grossly, DMBA-induced carcinogenesis started at the 9th week. Progressive signs appeared in the following weeks reaching to large ulcerative oral masses and exophytic nodules at the 21st week. Histologically, invasive well-differentiated oral squamous cell carcinoma (OSCC) appeared in the underlying tissues from the 12th week, showing malignant criteria. Genistein had delayed clinicopathological change, which started 6 weeks later, than the DMBA-painted hamsters, as mild epithelial dysplastic changes. This became moderate during the last 6 weeks, without dysplastic changes. Flow cytometry revealed that DMBA led to considerable variation in DNA proliferation activity, aneuploid DNA pattern, in 47.22% of hamsters and significantly raised the S-phase fragment (SPF) values, which drastically reduced after genistein treatment. Conclusion Taken together, genistein could be employed as an onco-suppressive agent for carcinogenesis. Moreover, FMA could be used as an aiding fast tool for diagnosis of cancer.

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“…In total, 60% of the leukoplakia cases were shown to have positive telomerase activity and were well correlated with the clinical type and histologic grade in leukoplakia. Other clinical types of leukoplakia were also reported to show positive telomerase activity with a rate of 31% in moderate to severe dysplasia, 44% in ulcerated and nodular, and 36% in verrucous leukoplakia ( Thomas et al, 2020 ). Another tumor marker, p53, was reported upregulated in leukoplakia, and approximately 1.6% of leukoplakia cases were p53 positive.…”

Section: Oral Potentially Malignant Disordersmentioning

confidence: 99%

“…A study conducted on genomic stability in the Indian population showed that aneuploidy levels in oral cancer and leukoplakia were 79.1% and 32.4% respectively. High-grade dysplastic cases of ulcerated, nodular, and verrucous leukoplakia have shown aneuploidy levels of 13.1, 22.2, and 28% respectively, whereas the diploid genome as reported in the majority (85%) of the non-dysplastic or mild dysplastic cases ( Thomas et al, 2020 ). Thus, it is evident that genomic content changes drastically during MT of leukoplakia, and monitoring these changes can provide important information for diagnosis and potential stratification of patients.…”

Section: Oral Potentially Malignant Disordersmentioning

confidence: 99%

Oral Potentially Malignant Disorders: Etiology, Pathogenesis, and Transformation Into Oral Cancer

2022

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Prognostic Implications of DNA Repair, Ploidy and Telomerase in the Malignant Transformation Risk Assessment of Leukoplakia

Abstract: Leukoplakia is defined as a white plaque of questionable risk having excluded other known disorders that carry no increased risk of cancer (Warnakulasuriya et al., 2007). Leukoplakia may be clinically categorised as homogenous

Cited by 4 publications

References 16 publications

Predictors of malignant transformation in oral leukoplakia and proliferative verrucous leukoplakia: An observational prospective study including the DNA ploidy status

Predictors of malignant transformation in oral leukoplakia and proliferative verrucous leukoplakia: An observational prospective study including the DNA ploidy status

Genistein anticancer efficacy during induced oral squamous cell carcinoma: an experimental study

Oral Potentially Malignant Disorders: Etiology, Pathogenesis, and Transformation Into Oral Cancer

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