Prognostic microRNAs associated with phosphoserine aminotransferase 1 in gastric cancer as markers of bone metastasis

Abstract

This study analyzed PSAT1-targeted miRNAs as a prognostic predictor for gastric cancer. The relationship between the clinical manifestations of gastric cancer in patients and phosphoserine aminotransferase 1 (PSAT1) was analyzed using correlation analysis. PSAT1 was highly expressed in gastric cancer, and its low expression was associated with a poor prognosis. By pan-cancer analysis, PSAT1 could affect the tumor immune microenvironment by immune infiltration analysis. Nine microRNAs targeting PSAT1 and associated with gastric cancer were screened by miRwalk and microRNA expression in TCGA tumor tissues. Six microRNAs were obtained by survival curve analysis, including hsa-miR-1-3p, hsa-miR-139-5p, hsa-miR-145-5p, hsa-miR-195-5p, hsa-miR-218-5p, and hsa-miR-497-5p. Based on the above six microRNAs, a model for bone metastasis prediction in gastric cancer prediction was constructed. An analysis of a decision curve was performed based on the microRNAs obtained to predict bone metastasis from gastric cancer. It had a positive area under the curve (AUC) value of 0.746, and the decision curve analysis (DCA) indicated that it was clinically significant. Dual-luciferase reporter genes indicated that hsa-miR-497-5p and PSAT1 were targeted, and qRT-PCR results confirmed that hsa-miR-497-5p could down-regulate PSAT1 expression. MicroRNAs targeting the regulation of PSAT1 expression can well predict the prognosis of gastric cancer.

Construct dysregulated miRNA-mRNA interaction networks to conjecture possible pathogenesis for Stomach adenocarcinomas

“…ANXA2 was included in a prognostic model containing 10 genes for gastric cancer [ 43 ]. MiR-1-3p were also absorbed in a model containing six miRNAs for bone metastasis prediction of gastric cancer [ 44 ]. Since detailed survival analyzes are often restricted by insufficient information collected in follow-up studies, the prognosis efficacy of the miRNA-mRNA axes needs to be further measured.…”

Exosomal microRNAs (miRNAs) in blood and urine under physiological conditions: a comparative study

“…Studies have found that photosensitive aminotransferase 1 (PSAT1) is a target gene for hsa-miR-218-5p and hsa-miR-145-5p, which can be used to predict bone metastasis in gastric cancer [17]. Frørup et al discovered that extracellular vesicles, particularly those enriched in the plasma exosomes of lactating mothers with Type 1 diabetes, contained abnormal levels of hsa-miR-30d-5p post-delivery [18].…”