Prognostic Role of T Helper 9 Cells and Interleukin-9 in Chronic Lymphocytic Leukemia

Sabry, Sabry A.; El-Send, Fardous F.; Abousam, Nashwa K.; El-D, Manal Salah; Youssef, Magdy M.

doi:10.3923/ajbmb.2018.23.33

Cited by 2 publications

(5 citation statements)

References 16 publications

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“…Detailed procedures of flow cytometric immunophenotyping analysis were performed as described in Sabry et al [11,12].…”

Section: Flow Cytometric Immunophenotypic Analysismentioning

confidence: 99%

“…FISH analysis was performed on peripheral blood samples from patients with CLL in Cytogenetics Laboratory in Oncology Center, Mansoura University using probes mentioned in a preceding paper [11].…”

Section: Cytogeneticsmentioning

confidence: 99%

“…Th9 cell level were defined as the number of IL-9 þ cells among T cell population that were CD5 þ CD19 À and after further characterization of cellular subtype by staining with antibodies to CD4 as mentioned in Sabry et al [11].…”

Section: Analysis Of the Changes In Circulating Th9 Cells By Flow Cytometrymentioning

confidence: 99%

“…The quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) analysis for quantification of IL-9 mRNA was done as defined previously by Lv et al [22] and mentioned by Sabry et al [11].…”

Section: Quantification Of Il-9 Mrna By Qrt-pcrmentioning

confidence: 99%

“…Blood samples were taken from patients and control groups and the serum was separated and stored at À20 C till analysis. The kit Human IL-9 DuoSet ELISA (R&D Systems) was used for estimating the level of IL-9 and performed according to instructions of the manufacturer and as mentioned in Sabry et al [11].…”

Section: Quantification Of Serum Level Of Il-9mentioning

confidence: 99%

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Oxidative stress in CLL patients leads to activation of Th9 cells: an experimental and comprehensive survey

Sabry

El-Senduny

Abousamra

et al. 2019

Immunological Medicine

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Older adults are mostly affected by chronic lymphocytic leukemia (CLL). The present study aimed to evaluate oxidative stress in CLL and to assess its impact on IL-9, Th9 cells levels and prognosis of cases. Seventy Egyptian CLL patients and 15 healthy controls were included. Th9 cell and immunophenotyping of abnormal B cells were assessed by flow cytometry, IL-9 level using ELISA, IL-9 mRNA by qRT-PCR, cytogenetics using FISH, and oxidative stress parameters were determined spectrophotometrically and with native gel electrophoresis. Oxidative stress was elevated in CLL that correlated with abnormal immunophenotyping, cytogenetic changes, bad prognosis, Th9 cells, and overexpression of IL-9. Levels of IL-9 and Th9 cells were strongly correlated with oxidative stress and bad prognostic markers in CLL, indicating that these cells may contribute to CLL by novel mechanisms that could include oxidant injury.

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“…Detailed procedures of flow cytometric immunophenotyping analysis were performed as described in Sabry et al [11,12].…”

Section: Flow Cytometric Immunophenotypic Analysismentioning

confidence: 99%

Section: Cytogeneticsmentioning

confidence: 99%

Section: Analysis Of the Changes In Circulating Th9 Cells By Flow Cytometrymentioning

confidence: 99%

Section: Quantification Of Il-9 Mrna By Qrt-pcrmentioning

confidence: 99%

Section: Quantification Of Serum Level Of Il-9mentioning

confidence: 99%