Prognostic significance of cell DNA content in early‐stage ovarian cancer (figo stages I and II/A) by means of automatic image cytometry

Wagner, Theresa; Adler, Anna; Sevelda, P.; Assmann, Ilka; Knepflé, Cecile F. H. M.; Czerwenka, K.; Heinzl, Harald; Ploem-Zaaijer, J. J.

doi:10.1002/ijc.2910560203

Cited by 12 publications

(8 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the early 1970s, DNA ploidy in ovarian cancer was suggested to be of prognostic impact. It has been confirmed in most studies that DNA content (DNA ploidy/ S-phase/DI/5C) is of prognostic value in patients with both early and advanced stage ovarian tumors as well as in those with borderline ovarian tumors (15,17,18,25,(29)(30)(31) , results that we could associate with recent EGFL7 expression studies (32) . In this study, unlike in the great majority of other studies, the percentage of early stage (I-II) cancers was higher than the advanced stage cancers.…”

Section: Discussionsupporting

confidence: 80%

DNA content evaluation for epithelial ovarian cancer identification

Tomé

et al. 2018

Curr. Gynecol. Oncol.

View full text Add to dashboard Cite

To assess the cellular DNA status of epithelial ovarian cancer cells for clinical stage identification and its effect on survival. Methods: Sixty-two patients treated by primary surgery and six courses of platinum-based chemotherapy were enrolled. The surgical stage was analyzed in correlation with DNA ploidy, S-phase fraction and DNA index. DNA analysis was performed via image cytometry. Results: From the 62 cases, 38 were International Federation of Gynecology and Obstetrics (Fédération Internationale de Gynécologie et d'Obstétrique, FIGO) stage I and II, 24-stage III and IV. In the DNA histograms obtained, the DNA index ranged from 0.85 to 3.02. Sixteen were classified as diploid and 46 as aneuploid (18 multiploid). S-phase fraction ranged from 9.8 to 51%. The aneuploid cells with DNA content above 5C ranged from 0.0 to 77.2%. Patients diagnosed with FIGO III and IV (vs. I and II) were 3.3 times more likely to die. Only in FIGO stage I and II the survival differed significantly for the different groups of ploidy. The risk of death for the multiploid (vs. diploid) group is 6.4 times and for aneuploid (vs. diploid) 2.3 times. Overall survival was better in the group with low DNA index. The low percentage compared with a high percentage of 5C cells ploidy groups showed association with mortality. The death hazard for the S-phase >33 median group is 4.9 times the hazard in relation to the S-phase <33. Conclusions: DNA ploidy, DNA index, S-phase, and 5C cells are important prognosticators for epithelial ovarian cancer mainly in early stages.

show abstract

Section: Discussionsupporting

confidence: 80%

DNA content evaluation for epithelial ovarian cancer identification

Tomé

et al. 2018

Curr. Gynecol. Oncol.

View full text Add to dashboard Cite

show abstract

“…The 2CDI is the mean square deviation between the DNA content in the selected cells and the diploid DNA content. Our laboratory also used the absolute number of cells with a DNA content of higher than 5C (N5C) in a cytological preparation with a given cell density 15,16 . This number can be corrected for polyploidy by subtracting the cells with a DNA content of 8C (N5C‐8C).…”

Section: Introductionmentioning

confidence: 99%

The suitability of DNA cytometry for the prediction of the histological diagnosis in women with abnormal cervical smears

Leeuwen

Ploem-Zaaijer

Pieters³

et al. 1996

BJOG

Self Cite

View full text Add to dashboard Cite

Objective To analyse the suitability of DNA cytometry for predicting the histological diagnosis in Design Survey with the use of diagnostic information to revise disease probability.Setting Colposcopy clinic of a university hospital.Participants One hundred and ten women with two mildly or moderately dyskaryotic cervical smears and 98 women with one severely dyskaryotic smear.Interventions DNA cytometric analysis using cytocentrifuge preparations of single cell suspensions from a cervical scrape. The main DNA cytometric parameter was N5C (i.e. the absolute number of cells with a DNA content of more than 5C on a given surface with a predefined cell density).Main outcome measure The probability of finding CIN I1 or worse. On arbitrary grounds, a positive test should point to a probability of 85 % or higher.Results In the patients with cervical neoplasia, the value of N5C increased significantly with an increasing CIN grade (P < 0.001). In the patients with one severely dyskaryotic smear and in those with two mildly or moderately dyskaryotic smears, the prior probability of finding CIN I1 or worse was 94 % and 53 %, respectively. Therefore, DNA cytometric analysis might be particularly useful in women with mild or moderate dyskaryosis ; further analysis was restricted to this group. All of the women in whom the N5C value was higher than 52 were diagnosed as having CIN I1 or worse. Only 16 (14.5%) of the 110 women had an N5C value of 52 or higher. When the N5C value was 27, the probability of finding CIN I1 or worse was estimated to be 85%. Only 28 (25%) patients had an N5C value of 27 or higher.Conclusions DNA cytometry produced significant diagnostic information, as was shown by the relation between N5C and the histological diagnosis. However, the N5C value could not discriminate sufficiently between women with CIN I1 or worse and CIN I or better. Therefore, the management of individual patients with cytological abnormalities cannot be based on the results of DNA cytometric analysis.women with cervical dyskaryosis.

show abstract

“…in rapidly progressive tumours compared with less aggressive malignancies (Miller et al, 1991). It may identify high-risk patients with ovarian cancer (Wagner et al, 1994). Rodenburg et al (1988) labelled hyperploid cells as 'marker cells' for malignant changes, such as increased genetic instability and impaired growth regulation.…”

Section: Discussionmentioning

confidence: 99%

Prognostic value of nuclear morphometry in patients with TNM stage T1 ovarian clear cell adenocarcinoma

et al. 1999

View full text Add to dashboard Cite

In 40 patients with TNM stage T1 ovarian clear cell adenocarcinoma, we used nuclear morphometry to study the relations among morphometric variables, clinical prognostic factors and outcome. The presence of one or more giant nuclear cells was positively associated with death (OR = 10.6, P = 0.02) and tended to be associated with disease recurrence (OR = 5.1, P = 0.07). Nuclear irregularity (expressed in terms of the nuclear roundness factor) was positively associated with both death (OR = 8.6, P = 0.02) and disease recurrence (OR = 8.2, P = 0.02). A combination of giant nuclear cell presence or nuclear irregularity proved to be a useful prognostic indicator, with a sensitivity and specificity of 83% and 71% in the prediction of death, and 75% and 71% in the prediction of disease recurrence. Patients' age and substage were of no prognostic value. We conclude that the nuclear morphometric characteristics, especially the presence of giant nuclear cells and nuclear irregularity, may be useful in predicting outcome in patients with early stage ovarian clear cell adenocarcinoma. © 1999 Cancer Research Campaign

show abstract

Prognostic significance of cell DNA content in early‐stage ovarian cancer (figo stages I and II/A) by means of automatic image cytometry

Cited by 12 publications

References 19 publications

DNA content evaluation for epithelial ovarian cancer identification

DNA content evaluation for epithelial ovarian cancer identification

The suitability of DNA cytometry for the prediction of the histological diagnosis in women with abnormal cervical smears

Prognostic value of nuclear morphometry in patients with TNM stage T1 ovarian clear cell adenocarcinoma

Contact Info

Product

Resources

About