Prognostic significance of DNA ploidy in oral squamous cell carcinomas

Baretton, Gustavo; Li, Xun; Stoll, Carola; Fischer-Brandies, E; Schmidt, Michael; Löhrs, U.

doi:10.1016/s1079-2104(05)80077-0

Cited by 30 publications

(7 citation statements)

References 31 publications

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“…In concordance with other studies, the results in table 2 demonstrate that the sensitivity for detection of aneuploidy is higher with image analysis than with single labelling flow cytometry [13][14][15][16][17]. There are several explanations for these findings.…”

Section: Comparison Of the Results Of Flow Cytometry And Image Analysissupporting

confidence: 89%

“…For determination of DNA content, image analysis is more established than single labelling flow cytometry and has a higher sensitivity for detecting aneuploid tumour cells [13][14][15][16][17]. For this method, only 250 tumour cell nuclei are needed and stromal cells can be excluded.…”

Section: Comparison Of Image Analysis and Flow Cytometry For Determinmentioning

confidence: 99%

“…At the least, tumour cells are diluted by a large amount of stromal cells. For image analysis the cells must be separated manually by an experienced pathologist [13][14][15][16][17]. Therefore, this method is time consuming.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, this method is time consuming. Flow cytometry is faster, but the sensitivity for detection of small amounts of aneuploid tumour cells is lower [13][14][15][16][17]. The aim of our study was to establish a flow cytometric method in order to pre-operatively determine the DNA content of oesophageal cancer cells from biopsy samples obtained during oesophago-gastro-duodenoscopy.…”

Section: Introductionmentioning

confidence: 99%

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Development of a Flow Cytometric Method to Determine DNA Ploidy of Oesophageal Cancer Cells Obtained by Forceps Biopsy Samples During Oesophago-Gastro-Duodenoscopy

Rickes¹,

Hauptmann

Flath³

et al. 2003

Oncol Res Treat

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Background: The DNA content of oesophageal tumour cells is a prognostic factor in untreated patients. To investigate whether DNA ploidy is useful to select patients for neoadjuvant therapy it is of interest to develop a method allowing reliable flow cytometric analysis of the DNA content of tumour cells obtained by forceps biopsy during endoscopy before start of therapy. Methods: Freshly frozen forceps biopsy samples from 30 patients with oesophageal cancer were disaggregated. DNA was stained with propidium iodide and ploidy was determined by flow cytometry. To enhance sensitivity epithelial cells were simultaneously labelled with anti-cytokeratin antibodies. Results were compared with image analysis. To evaluate the sampling error, parallel measurements were done in 10 patients by image analysis on forceps biopsies obtained during endoscopy before surgery and on the resected tumour. Results: The sensitivity to detect aneuploidy was lower for standard flow cytometry than for image analysis (13 versus 33%). The overall sensitivities were identical using a double labelling technique with additional cytokeratin-staining of the epithelial cells, but divergent results were obtained in 2 cases, where detection of aneuploidy was either possible with image analysis or with double labelling flow cytometry only. DNA content of samples gained by forceps biopsies and surgically resected tumours was concordant in 8 of 10 cases. In 2 patients, aneuploidy was detected only in the surgically resected tumour but not in the pre-operatively obtained forceps biopsies. Conclusions: A flow cytometric method for routine determination of the DNA ploidy of cells obtained by forceps biopsies from patients with oesophageal cancer was developed and evaluated against image analysis. The technique allows the prediction of DNA content before tumour resection, and might be used for optimising therapy and the patient’s quality of live.

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Section: Comparison Of the Results Of Flow Cytometry And Image Analysissupporting

confidence: 89%

Section: Comparison Of Image Analysis and Flow Cytometry For Determinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of a Flow Cytometric Method to Determine DNA Ploidy of Oesophageal Cancer Cells Obtained by Forceps Biopsy Samples During Oesophago-Gastro-Duodenoscopy

Rickes¹,

Hauptmann

Flath³

et al. 2003

Oncol Res Treat

View full text Add to dashboard Cite

show abstract

“…Data were analyzed using Modfit software (Becton-Dickinson (B-D) Sunnyvale, CA). Interpretation of DNA histograms was done according to Das et al [14] and Baretton et al [15]. Histograms showing a single G0/G1 peak were defined as diploid.…”

Section: Dna Flow Cytometrymentioning

confidence: 99%