Prognostic Significance of E-cadherin Expression and Peripheral Blood Micrometastasis in Gastric Carcinoma Patients

Saad, Abeer A.; Awed, Nahla M.; Elkerim, Nashwa N. A. Abd; El‐Shennawy, Dina; Alfons, Marian A.; Elserafy, Mohamed E.; Darwish, Yaser W.; Barakat, Eman; Ezz-Elarab, Sahar S.

doi:10.1245/s10434-010-1151-8

Cited by 32 publications

(31 citation statements)

References 15 publications

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“…The major cause of death from GC is the inability to detect and prevent metastasis at an early stage of disease. Several studies investigating the presence of CTCs in gastric cancer patients have been reported in the literature, but both the CTC detection techniques used and the results obtained were heterogeneous [15–17]. And most of these studies focused on the sensitivity and specificity of CTCs isolation, as well as the relationship between CTCs and cancer relapse.…”

Section: Discussionmentioning

confidence: 99%

Stem Cell-Like Circulating Tumor Cells Indicate Poor Prognosis in Gastric Cancer

Zhang

et al. 2014

BioMed Research International

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Circulating tumor cells (CTCs), which have stem cell-like characteristics, might play a crucial role in cancer metastasis. CD44 has been identified as gastric cancer (GC) stem cell (CSC) marker. Here, the prognostic significance of CD44-positive CTCs in GC patients was investigated. CTCs were detected in 27 of 45 GC patients. The presence of CTCs was significantly associated with lymph node metastasis, distant metastasis, and recurrence (P = 0.007, P = 0.035, and P = 0.035, resp.). Nineteen of the 27 CTC-positive patients had CD44-positive CTCs. These patients were more likely to develop metastasis and recurrence than patients with CD44-negative CTCs. CD44-positive CTC counts were higher in recurrent patients than in the nonrecurrent ones (means 4.8 and 1.9, resp.; P = 0.010). Furthermore, 13 of 19 patients with CD44-positive CTCs developed recurrent disease, and the mean time to recurrence was shorter than that in patients with CD44-negative CTCs (10.54 ± 5.55 and 19.13 ± 9.72 months, resp.; P = 0.04). COX proportional hazards model indicated that the presence of CD44-positive CTCs and TNM stage were independent predictors of recurrence for GC (P = 0.030 and 0.008). So identifying the stem cell-like CTC subset may provide more clinically useful prognostic information than only detecting CTCs.

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Section: Discussionmentioning

confidence: 99%

Stem Cell-Like Circulating Tumor Cells Indicate Poor Prognosis in Gastric Cancer

Zhang

et al. 2014

BioMed Research International

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“…Snail bind to E-boxes in the E-cadtherin promoter to repress gene transcription and induce cell migration and invasion[32]. In many human cancers, there is an inverse relationship between E-cadherin and Snail expression[9, 13, 18, 36-39]. Although Snail was associated with invasion and lymph node metastasis in many human cancers; the role of Snail in SOD2-induced TSCC invasion had not been evaluated.…”

Section: Discussionmentioning

confidence: 99%

Manganese superoxide dismutase induces migration and invasion of tongue squamous cell carcinoma via H2O2-dependent Snail signaling

Su²,

Cai³

et al. 2012

Free Radical Biology and Medicine

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Our previous studies had revealed that the dysregulation of manganese superoxide dismutase (SOD2) expression was a frequent event in tongue squamous cell carcinoma (TSCC) and may be associated with enhanced metastatic potential. To further evaluate the mechanism of SOD2-mediated metastasis in TSCC, TSCC cell lines with different metastatic potential (i.e., the highly metastatic UM1 line and the UM2 line, which displays fewer metastases) were used. Compared to UM2 cells, UM1 cells exhibited significantly higher SOD2 activity and intracellular H2O2, higher protein levels of Snail, MMP-1 and pERK1/2, lower protein levels of E-cadtherin, and not difference of catalase activity. Upon knockdown of SOD2 by RNA interference, UM1 cells displayed significantly reduced migration and invasion abilities, reduced activities of SOD2, lower intracellular H2O2, decreased protein levels of Snail, MMP-1 and pERK1/2, and increased protein levels of E-cadtherin. Migration and invasion ability of UM2 and SOD2 shRNA-transfected UM1 cells were enhanced by H2O2 treatment and accompanied by increased protein levels of Snail, MMP-1 and pERK1/2, and decreased protein levels of E-cadtherin. Moreover the migration and invasion ability of UM1 cells were decreased after catalase treatment. Thus, we conclude that the SOD2-dependent production of H2O2 contributes to both the migration and invasion of TSCC via the Snail signaling pathway through increased Snail, MMP-1 and pERK1/2 protein levels, and the repression of the E-cadtherin protein.

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“…The presence of CTCs in circulation suggests not only a high risk of tumor recurrence, but also an unfavorable clinical outcome even in the early stages of GC [20]. The prognostic impact of CTCs in GC has been reported in several studies [21][22][23][24][25][26][27]. The sensitivity of RT-PCR CTC detection was superior to the other less commonly used cytological detection methods involving fluorescence-activated cell sorting (FACS), immunohistochemistry (IHC), and immunocytochemistry (ICC) [20].…”

Section: Gastric Cancer (Gc)mentioning

confidence: 98%

Clinical studies monitoring circulating and disseminated tumor cells in gastrointestinal cancers

Eliasova

Kološtová

Kobierzycki

et al. 2014

Folia Histochem Cytobiol.

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Abstract:Circulating tumor cells (CTCs) and disseminated tumor cells (DTCs) are responsible for the development of metastatic disease, and may also hold the key to determining tailored therapies of advanced cancer disease. Our review summarizes the prognostic significance of the detection of CTCs and DTCs in various gastrointestinal cancers with an overview of their possible use as prognostic biomarkers. This could be used in the future as a starting point for new clinical trials focusing on the predictive potential of circulating and disseminated tumor cells. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 4, 265-277) Key words: circulating tumor cells; gastrointestinal cancer; esophageal cancer; colorectal cancer; gastric cancer; plastin3; prognosis Abbreviations AFP -alpha fetoprotein; BM -bone marrow; CD -cluster of differentiation; CEA -carcinoembryonic antigen; CHT -chemotherapy; CI -confidence interval; CTC -circulating tumor cell; CRCcolorectal carcinoma; CVB -central venous blood; CK -cytokeratin; DAPI -4,6-diamidino-2-phenylindole; DFS -disease free survival; DTC -disseminated tumor cell; EpCAM -epithelial cell adhesion molecule; FISH -fluorescent in situ hybridization; 5-FU -5-fluorouracil; HCC -hepatocellular carcinoma; HR -hazard ratio; ISET -isolation by size of epithelial tumor; ITC -isolated tumor cells; MACS -magnetic activated cell sorting; MFS -metastasis free survival; MSP -methylation specific polymerase chain reaction; MVB -mesenteric venous blood; NA -not available; OS -overall survival; PB -peripheral blood; PFS -progression-free survival; qPCR -quantitative real-time polymerase chain reaction; RFA -radiofrequency ablation; RT -radiotherapy; RT-PCR -reverse transcription polymerase chain reaction; TGFb1 -transforming growth factor b1; TRC method -transcription reverse-transcription concerted method

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Prognostic Significance of E-cadherin Expression and Peripheral Blood Micrometastasis in Gastric Carcinoma Patients

Cited by 32 publications

References 15 publications

Stem Cell-Like Circulating Tumor Cells Indicate Poor Prognosis in Gastric Cancer

Stem Cell-Like Circulating Tumor Cells Indicate Poor Prognosis in Gastric Cancer

Manganese superoxide dismutase induces migration and invasion of tongue squamous cell carcinoma via H2O2-dependent Snail signaling

Clinical studies monitoring circulating and disseminated tumor cells in gastrointestinal cancers

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