Prognostic significance of galectin-1 expression in patients with cancer: a meta-analysis

Wu, Rongzu; Wu, Tingchun; Wang, Kai; Luo, Shicheng; Chen, Zhe; Fan, Min; Xue, Dong; Lü, Hao; Zhuang, Qianfeng; Xu, Xianlin

doi:10.1186/s12935-018-0607-y

Cited by 27 publications

(22 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Galectin-1 (LGALS1) is a member of the carbohydrate-binding protein family, which shares a conserved amino acid sequence in the carbohydrate recognition domain and shows high affinity for β-galactoside-containing glycol-conjugates [5,6]. Several studies have demonstrated that LGALS1 plays an important role in tumorigenesis, progression and metastasis of human cancers [7], serving as a reliable diagnostic and prognostic biomarker in cancerous patients [8,9]. For instance, Schulkens et al [10] have observed that LGALS1 expression in non-small cell lung cancer tissues was elevated compared with adjacent normal tissues.…”

Section: Introductionmentioning

confidence: 99%

Effects of Galectin-1 on Biological Behavior in Cervical Cancer

Chetry¹,

Song²,

Pan³

et al. 2020

J. Cancer

View full text Add to dashboard Cite

Background:We previously revealed that the expression of galectin-1 (LGALS1) was significantly reduced after neoadjuvant chemotherapy treatment in cervical cancer patients. The objective of this study is to investigate the effects of LGALS1 expression on biological behaviors of cervical cancer cells. Methods: Immunohistochemistry and immunocytochemistry were performed to detect the expression of LGALS1 in cervical cancer tissues and cells (SiHa and C33A). Western blot analysis was performed to evaluate the efficacy of lentivirus-mediated upregulation or downregulation of LGALS1 in cervical cancer cells. Cell viability and proliferation were detected by CCK-8 and BrdU assays, respectively. Annexin V-FITC/PI apoptosis detection kit was employed to measure the apoptosis of cervical cancer cells. Transwell invasion and migration assays were also conducted to explore the invasive and migratory capabilities of cervical cancer cells. The expression of apoptosis-(Bcl-2 and Bax), invasion-(MMP-2 and MMP-9), and migration-related (Fascin and Ezrin) proteins, were detected by Western blot analysis. Xenograft mouse model of cervical cancer was generated to explore whether LGALS1 overexpression could promote tumor growth in vivo. Results:LGALS1 was overexpressed in cervical cancer tissues and cell lines compared to that in normal cervical tissues and epithelium cells. Upregulation of LGALS1 significantly promoted the cell proliferation, inhibited cell apoptosis, and enhanced the migratory and invasive abilities of both SiHa and C33A cells, whereas downregulation of LGALS1 led to the opposite results. The level of Bcl-2, MMP-2, MMP-9, Fascin, and Erzin expression was significantly upregulated in cervical cancer cells with LGALS1 overexpression, while converse results were obtained in LGALS1 knockdown cancer cells. In vivo study also showed that LGALS1 overexpression facilitated tumor growth of cervical cancer cells. Conclusion: Overexpression of LGALS1 significantly promoted and enhanced the aggressive features of cervical cancer both in vitro and in vivo, which may be associated with high expression of Bcl-2, MMP-2, MMP-9, Fascin, and Erzin proteins.

show abstract

Section: Introductionmentioning

confidence: 99%

Effects of Galectin-1 on Biological Behavior in Cervical Cancer

Chetry¹,

Song²,

Pan³

et al. 2020

J. Cancer

View full text Add to dashboard Cite

show abstract

“…Briefly, MG‐63 cells were grown in 60 mm cell culture dishes, treated with 6n for 48 hr and the supernatant was collected. As Gal‐1 is an extracellular secreted protein, its expression can be quantified from the cellular external environment (Wu et al., ). Equal amounts of supernatant were subjected to quantitative enzyme immunoassay as per manufacture's protocol (DGAL10, R&D Systems).…”

Section: Resultsmentioning

confidence: 99%

Synthesis and biological evaluation of morpholines linked coumarin–triazole hybrids as anticancer agents

et al. 2019

View full text Add to dashboard Cite

A series of novel morpholines linked coumarin–triazole hybrids (6a–6v) has been synthesized and evaluated for their anti‐proliferative potential on a panel of five human cancer cell lines, namely bone (MG‐63), lung (A549), breast (MDA‐MB‐231), colon (HCT‐15) and liver (HepG2), using MTT assay. Among all, the compound 6n {7‐((1‐(2,4‐dichlorobenzyl)‐1H‐1,2,3‐triazol‐4‐yl) methoxy)‐4‐((2,6‐dimethylmorpholino) methyl)‐2H‐chromen‐2‐one} showed significant growth inhibition against MG‐63 cells with an IC50 value of 0.80 ± 0.22 μM. Further, induction of apoptosis by 6n of MG‐63 cells confirmed as a result of morphological changes, the sub‐G1 phase arrest, increased percentage of apoptotic cells, and decrease in mitochondrial membrane potential and increase in reactive oxygen species levels. The in vitro Gal‐1 expression in cell culture supernatant of MG‐63 cells treated with compound 6n showed dose‐dependent reduction. The binding constant (Ka) of 6n with Gal‐1 was calculated from the intercept value which was observed as 3.0 × 105 M−1 by fluorescence spectroscopy. Surface plasmon resonance showed that 6n binds to Gal‐1 with binding constant (Ka) of 1.29E+04 1/Ms and equilibrium constant KD value of 7.54E−07 M, respectively. Molecular docking studies revealed the binding interactions of 6n with Gal‐1.

show abstract

“…There are many similar and recent studies that support our findings. Wu [19] observed that high galectin-1 expression was associated with poor OS in digestive cancers. Wang [20] suggested that galectin-3 plays an oncogenic role, and is expressed in colorectal cancer, non-small cell lung cancer and ovarian cancer.…”

Section: Discussionmentioning

confidence: 99%

Prognostic role of galectins expression in patients with hepatic cancer

Shao

Chen

et al. 2020

Medicine

View full text Add to dashboard Cite

Objective: The objective of this study was to illustrate the prognostic value of diversified galectins in patients with hepatic cancer via meta-analysis. Methods: We conducted a systematic search on PubMed, Embase, The Cochrane Library, Web of Science, the Chinese National Knowledge Infrastructure (CNKI) database, and Wanfang Data for studies that reported associations between galectin expression and the prognosis for hepatic cancer patients, from the inception of each database to March 20, 2019. The combined hazard ratio (HR) and 95% confidence interval (CI) were estimated to investigate the prognosis. Results: We collected 11 studies of 1957 patients in our meta-analysis. The pooled results indicated that overall galectin expression was not correlated with OS (HR = 1.23, 95% CI = 0.84–1.79, P = .29) or DFS/RFS (HR = 0.808, 95% CI = 0.376–1.735, P = .42) in liver cancer patients. In stratified analyses, we observed that high galectin-1 and galectin-3 expression was significantly associated with poor OS. The pooled HR of galectin-4 and galectin-9 was correlated with improved OS. Conclusion: Our results indicate that the high expression of galectin-1 and -3 and the low expression of galectin-4 and -9 may be predictive prognostic factors for poor OS in liver cancer patients.

show abstract

Prognostic significance of galectin-1 expression in patients with cancer: a meta-analysis

Cited by 27 publications

References 37 publications

Effects of Galectin-1 on Biological Behavior in Cervical Cancer

Effects of Galectin-1 on Biological Behavior in Cervical Cancer

Synthesis and biological evaluation of morpholines linked coumarin–triazole hybrids as anticancer agents

Prognostic role of galectins expression in patients with hepatic cancer

Contact Info

Product

Resources

About