Prognostic Significance of Intratumour Microvessel Density and Haemoglobin Level in Carcinoma of the Uterine Cervix

Gasińska, Anna; Urbański, Krzysztof; Adamczyk, Agnieszka; Pudełek, Jacek; Lind, Bengt K.; Brahme, Anders

doi:10.1080/028418602320405023

Cited by 19 publications

(15 citation statements)

References 34 publications

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“…Our preliminary experiments showed that brain tumors did not have "hot spots" [sites of vessel concentration, see [26]]. Therefore, the vessels were counted using global approach [26,27].…”

Section: Vessel Density and Area Measurementmentioning

confidence: 99%

Inhibition of laminin-8 in vivo using a novel poly(malic acid)-based carrier reduces glioma angiogenesis

et al. 2006

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We have previously shown that laminin-8, a vascular basement membrane component, was overexpressed in human glioblastomas multiforme and their adjacent tissues compared to normal brain. Increased laminin-8 correlated with shorter glioblastoma recurrence time and poor patient survival making it a potential marker for glioblastoma diagnostics and prediction of disease outcome. However, laminin-8 therapeutic potential was unknown because the technology of blocking the expression of multi-chain complex proteins was not yet developed. To inhibit the expression of laminin-8 constituents in glioblastoma in vitro and in vivo, we used Polycefin, a bioconjugate drug delivery system based on slime-mold Physarum polycephalum-derived poly(malic acid). It carries an attached transferrin receptor antibody to target tumor cells and to deliver two conjugated morpholino antisense oligonucleotides against laminin-8 α4 and β1 chains. and area (p < 0.001) and increased animal survival (p < 0.0004). These data suggest that laminin-8 may be important for glioblastoma angiogenesis. Polycefin, a versatile nanoscale drug delivery system, was suitable for in vivo delivery of two antisense oligonucleotides to brain tumor cells causing a reduction of glioblastoma angiogenesis and an increase of animal survival. This system may hold promise for future clinical applications.

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“…Our preliminary experiments showed that brain tumors did not have "hot spots" [sites of vessel concentration, see [26]]. Therefore, the vessels were counted using global approach [26,27].…”

Section: Vessel Density and Area Measurementmentioning

confidence: 99%

Inhibition of laminin-8 in vivo using a novel poly(malic acid)-based carrier reduces glioma angiogenesis

et al. 2006

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“…Further research is needed to identify more effective prognostic markers for the improvement of the clinical management of cervical carcinoma patients. This would also support the development of novel individually tailored strategies for use in the treatment of high-risk patients due to molecular risk factors (3)(4)(5)(6).…”

Section: Introductionmentioning

confidence: 97%

Overexpression of astrocyte-elevated gene-1 is associated with cervical carcinoma progression and angiogenesis

et al. 2013

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Astrocyte-elevated gene-1 (AEG-1) is implicated in the oncogenesis and angiogenesis of various types of human cancers. However, the biological roles of AEG-1 in cervical carcinoma remain to be further elucidated. In the present study, we demonstrated that the expression of AEG-1 was markedly upregulated in the cervical carcinoma cell lines HeLa, CaSki and SiHa, as well as in 8 paired primary cervical carcinoma tissue (CCT) specimens at both the transcriptional and translational levels when compared with normal cervical epithelial cells (NCECs). Furthermore, immunohistochemical (IHC) analysis demonstrated that 180 of 200 (90%) archived CCT specimens exhibited positive staining for AEG-1, and statistical analysis revealed that the upregulation of AEG-1 was significantly correlated with the clinical staging of the patients (P=0.034), including T (P=0.019), N (P=0.038) and M classification (P=0.018) as well as tumor differentiation (P=0.043). Furthermore, loss‑ and gain‑of‑function results showed that knockdown of AEG-1 expression by specific shRNA not only inhibited SiHa cell proliferation and invasive ability, but also significantly decreased the expression of the angiogenesis-related genes HIF-1α, Tie2, VEGF and TEM1/CD248. Moreover, an increased vascular formation ability was observed in human umbilical vein endothelial cells (HUVECs) co-cultured with conditioned medium both from SiHa cells and NCECs transfected with ectopic AEG-1. In conclusion, these results suggest that elevated expression of AEG-1 plays an important role in the aggressiveness and angiogenesis of cervical carcinoma and that AEG‑1 represents a novel and valuable predictive factor for the prognostic evaluation of cervical carcinoma patients.

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“…Although many clinicopathological parameters including clinical or pathological stage of disease, tumor volume or size, lymphatic and metastatic status, and tumor microvessel intensity have been reported to be associated with prognosis, it is still necessary to search so better prognostic markers to improve clinical management of cervical carcinoma patients (Kawagoe et al 1999;Martin-Loeches et al 2002;Gasinska et al 2002). The advent of genomic and proteomic technologies has made it possible to identify novel biomarkers which can predict the presence and prognosis of cancers.…”

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confidence: 99%

Expression of stathmin/op18 as a significant prognostic factor for cervical carcinoma patients

Wang

Lin

et al. 2008

J Cancer Res Clin Oncol

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Prognostic Significance of Intratumour Microvessel Density and Haemoglobin Level in Carcinoma of the Uterine Cervix

Cited by 19 publications

References 34 publications

Inhibition of laminin-8 in vivo using a novel poly(malic acid)-based carrier reduces glioma angiogenesis

Inhibition of laminin-8 in vivo using a novel poly(malic acid)-based carrier reduces glioma angiogenesis

Overexpression of astrocyte-elevated gene-1 is associated with cervical carcinoma progression and angiogenesis

Expression of stathmin/op18 as a significant prognostic factor for cervical carcinoma patients

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