Prognostic significance of RelB overexpression in non‐small cell lung cancer patients

Abstract

BackgroundLung cancer is a major public health issue in most countries, including China. The expression of RelB is associated with poor prognosis in diverse cancers. However, whether RelB expression could be an indicator of poor prognosis in non‐small cell lung cancer (NSCLC) is still unclear.MethodsThe expression of RelB in NSCLC tumor tissue and adjacent non‐neoplastic tissues were examined by immunohistochemistry. Chi‐square or two‐tailed Fisher's exact tests were used to analyze possible associations between qualitative clinicopathological variables and RelB expression. Kaplan–Meier analysis and a Cox regression model were employed to determine independent prognostic factors.ResultsThe expression of RelB was increased in tumor tissue compared with adjacent non‐neoplastic tissue in NSCLC patients. High RelB expression was significantly correlated with degree of differentiation (P = 0.023), depth of tumor invasion (P < 0.001), lymph node metastasis (P = 0.017), distant metastases (P = 0.004), and tumor node metastasis stage (P < 0.001) in patients with NSCLC. NSCLC patients with high RelB expression had significantly shorter overall survival than those with low RelB expression (P < 0.001). Our results indicate that high RelB expression is an independent prognostic factor for patients with NSCLC (P < 0.001).ConclusionsHigh RelB expression could provide a basis for judgment of prognosis in patients with NSCLC.

Differential impact of classical and non-canonical NF-κB pathway-related gene expression on the survival of breast cancer patients

“…Also, patients with higher expression of RELB had decreased RFS and OS in ER-positive breast carcinoma [67]. Moreover, in other tumors such as lung cancer, the expression of NIK and RELB was associated with metastasis and shorter OS [68,69]. These studies are contrary to what we have observed regarding NIK and RELB mRNA expression.…”

Co‐expression of NF‐κB‐p65 and phosphorylated NF‐κB‐p105 is associated with poor prognosis in surgically resectable non‐small cell lung cancer

“…We noticed that some clinical studies reporting NF-jB expression detected by IHC that they correlated with clinicopathological characteristics and/or survival in lung cancers had conflicting results; summarized in Table 3 [7][8][9][10][11][12][13][14][15]. The Rel protein family members can form up to 15 different dimers through combinatorial associations, and various antibodies for different key NF-jB pathway components, such as p65, p105, IjBa and IKKa/b, were used for the evaluation of NF-jB expression in the different studies [6].…”

“…The same series of studies have correlated NF-jB expression with clinicopathological characteristics and survival, but the results have been inconsistent. In addition, in most studies, pathologic studies of NF-jB expression were detected by assaying for single components of the NF-jB pathway, and using different anti-NF-jB subunit antibody clones, judgement criteria and cut-off values for assessing NF-jB expression were used in the various studies [6][7][8][9][10][11][12][13][14][15]. Actually, different NF-jB dimers may have different functions, especially the p50-p50 homodimer, which lacks a transactivation domain in the C-termini and has no intrinsic ability to activate transcription.…”

Exopolysaccharides from a Codonopsis pilosula endophyte activate macrophages and inhibit cancer cell proliferation and migration

“…Cells on the upper surface of the inserts were completely wiped off with a cotton swab, while cells that migrated to the underside of the inserts were fixed with 4% paraformaldehyde for 15 minutes at room temperature. Cells were then stained with 0.5% crystal violet in 20% methanol for 30 minutes, washed three times with distilled water, and visualized with a Leica stereomicroscope (Leica Microsystems, Wetzlar, Germany) . For wound healing assay, cells were grown to 90% confluence in a 12‐well plate, and a scratch wound was created in the monolayer using a pipette tip.…”