Prognostic significance of TEM7 and nestin expression in women with advanced high grade serous ovarian cancer

Czekierdowski, Artur; Stachowicz, Norbert; Czekierdowska, Sylwia; Łoziński, Tomasz; Gurynowicz, Grzegorz; Kluz, Tomasz

doi:10.5603/gp.a2018.0023

Cited by 10 publications

(4 citation statements)

References 22 publications

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“…Similarly, Plxdc1 was first described in a screen for novel tumor endothelial members [25] and expression of both Plxdc1 and Plxdc2 is elevated in the endothelium of solid tumors [25,26,46,47]. Plxdc1 expression has been described as prognostic marker and modulating factor for various human cancers [46][47][48][49][50]. Given the overlap between the required changes e.g.…”

Section: Plos Pathogensmentioning

confidence: 99%

Plxdc family members are novel receptors for the rhesus monkey rhadinovirus (RRV)

et al. 2021

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The rhesus monkey rhadinovirus (RRV), a γ2-herpesvirus of rhesus macaques, shares many biological features with the human pathogenic Kaposi’s sarcoma-associated herpesvirus (KSHV). Both viruses, as well as the more distantly related Epstein-Barr virus, engage cellular receptors from the Eph family of receptor tyrosine kinases (Ephs). However, the importance of the Eph interaction for RRV entry varies between cell types suggesting the existence of Eph-independent entry pathways. We therefore aimed to identify additional cellular receptors for RRV by affinity enrichment and mass spectrometry. We identified an additional receptor family, the Plexin domain containing proteins 1 and 2 (Plxdc1/2) that bind the RRV gH/gL glycoprotein complex. Preincubation of RRV with soluble Plxdc2 decoy receptor reduced infection by ~60%, while overexpression of Plxdc1 and 2 dramatically enhanced RRV susceptibility and cell-cell fusion of otherwise marginally permissive Raji cells. While the Plxdc2 interaction is conserved between two RRV strains, 26–95 and 17577, Plxdc1 specifically interacts with RRV 26–95 gH. The Plxdc interaction is mediated by a short motif at the N-terminus of RRV gH that is partially conserved between isolate 26–95 and isolate 17577, but absent in KSHV gH. Mutation of this motif abrogated the interaction with Plxdc1/2 and reduced RRV infection in a cell type-specific manner. Taken together, our findings characterize Plxdc1/2 as novel interaction partners and entry receptors for RRV and support the concept of the N-terminal domain of the gammaherpesviral gH/gL complex as a multifunctional receptor-binding domain. Further, Plxdc1/2 usage defines an important biological difference between KSHV and RRV.

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Section: Plos Pathogensmentioning

confidence: 99%

Plxdc family members are novel receptors for the rhesus monkey rhadinovirus (RRV)

et al. 2021

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“…The study by Onisim et al ( 43 ) showed that the expression of nestin in tumour cells was associated with poorer PFS and OS in patients with ovarian cancer. In another study by Czekierdowski et al ( 84 ) it was found that in high grade serous ovarian cancer patients with high expression of nestin had worse OS and DFS rates than patients with low expression of nestin. Qin et al ( 85 ) found that in serous ovarian cancer nestin-positive patients had significantly shorter OS.…”

Section: Clinical Significance Of Cscs Markers In Ovarian Cancermentioning

confidence: 95%

“…The surface markers include: CD133, CD44, CD117, CD24, CD105, CD106, CD90, SSEA1 and EpCAM. The intracellular markers include: ALDH1, SOX2 and Nestin ( 39 , 52 , 84 , 90 ). SSEA1, stage-specific embryonic antigen-1; EpCAM, epithelial cell adhesion molecule; ALDH1, aldehyde dehydrogenase 1; SOX2, sex-determining region Y-box 2.…”

Section: Figurementioning

confidence: 99%

Cancer stem cells markers in ovarian cancer: Clinical and therapeutic significance (Review)

Królewska-Daszczyńska

Wendlocha

Smycz‐Kubańska

et al. 2022

Oncol Lett

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Ovarian cancer is a gynecological neoplasm that can be found in women, which, due to diagnostic difficulties, is often detected at advanced stages when treatment becomes a significant problem. Moreover, in a number of cases there is a cancer recurrence and resistance to standard chemotherapy treatment. It has been suggested that cancer stem cells (CSCs) that were not eradicated during therapy may be responsible for this. For this reason, effective therapeutic methods eliminating CSCs are being studied, such as therapy targeting CSCs markers. In addition, numerous studies have also drawn attention to the usefulness of CSCs markers in predicting disease progression and assessing patient's prognosis as well as their importance in the development of treatment resistance. The present review presented research on selected CSCs markers, which may be of significant prognostic and therapeutic importance in ovarian cancer. Contents1. Introduction 2. Cancer stem cells (CSCs) 3. CSCs in ovarian cancer 4. Clinical significance of CSCs markers in ovarian cancer 5. Therapeutic importance of CSCs markers 6. Conclusion PATRYCJA KRÓLEWSKA-DASZCZYŃSKA, DOMINIKA WENDLOCHA, MARTA SMYCZ-KUBAŃSKA, SEBASTIAN STĘPIEŃ and ALEKSANDRA MIELCZAREK-

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“…Finally, the fact that a mutant that was deleted in both the Eph and the Plxdc1/2 interaction motif, RRV-YFP gH∆21-27-AELAAN, was still able to replicate on rhesus monkey fibroblasts, as evidenced by the fact that we were able to grow a virus stock on these cells, and was still infectious to a certain degree on a number of cell lines ( Similarly, Plxdc1 was first described in a screen for novel tumor endothelial members (25) and expression of both Plxdc1 and Plxdc2 is elevated in the endothelium of solid tumors (25,26,42,43). Plxdc1 expression has been described as prognostic marker and modulating factor for various human cancers (42)(43)(44)(45)(46). Given the overlap between the required changes e.g.…”

Section: Ebv and Hcmv A Clear Correlation Between Receptor Usage Dementioning

confidence: 99%

Plxdc family members are novel receptors for the rhesus monkey rhadinovirus (RRV)

Großkopf

Schlagowski

Ensser

et al. 2020

Preprint

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10The rhesus monkey rhadinovirus (RRV), a γ2-herpesvirus of rhesus macaques, shares many biological 11 features with the human pathogenic Kaposi's sarcoma-associated herpesvirus (KSHV). Both viruses, 12 as well as the more distantly related Epstein-Barr virus, engage cellular receptors from the Eph family 13 of receptor tyrosine kinases (Ephs). However, the importance of the Eph interaction for RRV entry 14 varies between cell types suggesting the existence of Eph-independent entry pathways. We 15 therefore aimed to identify additional cellular receptors for RRV by affinity enrichment and mass 16 spectrometry. We identified an additional receptor family, the Plexin domain containing proteins 1 17 and 2 (Plxdc1/2) that bind the RRV gH/gL glycoprotein complex. In vitro, blocking assays with soluble 18Plxdc2 decoy receptor reduced RRV infection by approx. 60%, while overexpression of Plxdc1 and 2 19 dramatically enhanced RRV susceptibility in otherwise marginally permissive Raji cells. While the 20 Plxdc2 interaction is conserved between two RRV strains, 26-95 and 17577, Plxdc1 specifically 21 interacts with RRV 26-95 gH. The Plxdc interaction is mediated by a short motif at the N-terminus of 22 RRV gH that is partially conserved between isolate 26-95 and isolate 17577, but absent in KSHV gH. 23Mutation of this motif abrogated the interaction with Plxdc1/2 in in vitro assays and reduced RRV 24 infection in a cell-type specific manner. Taken together, our findings characterize Plxdc1/2 as novel 25 interaction partners and entry receptors for RRV and support the concept of the N-terminal domain 26 of the gammaherpesviral gH/gL complex as a multifunctional receptor-binding domain. 27 vivo studies. Two major RRV sequence groups have been identified (4), which are represented by two 33 cloned isolates, RRV 26-95 (5) and RRV 17577 (6). Analogous to KSHV infection, primary RRV infection 34 is asymptomatic in healthy hosts and leads to life-long persistence, most likely in the B cell 35 compartment (7). KSHV is associated with a solid tumor of endothelial origin, Kaposi's sarcoma (KS), 36 and two B cell malignancies, primary effusion lymphoma (PEL) and the plasmablastic variant of 37 multicentric Castleman's disease (MCD), most prominently in the context of human 38 immunodeficiency virus (HIV) infection and in immunocompromised individuals. Similarly, simian 39 immunodeficiency virus (SIV)-positive rhesus macaques developed B cell lymphomas upon 40 experimental infection with RRV strain 17577 (8, 9) and several studies correlated RRV infection with 41 lymphomagenesis in SIV/SHIV-infected animals (10, 11). While RRV is not consistently associated 42 with solid malignancies, RRV has been identified in retroperitoneal fibromatosis tissue (9, 12), similar 43 to retroperitoneal fibromatosis herpesvirus (RFHV)(11). Another shared characteristic of KSHV and 44 RRV is the receptor usage on a range of cell types. Both viruses engage members of the Eph family of 45 receptor tyrosine kinases (Ephs) through their glycoprotein (g)H...

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Prognostic significance of TEM7 and nestin expression in women with advanced high grade serous ovarian cancer

Abstract: Nestin as a marker of cancer stem cells may assist in the prediction of OS and DFS in women with high grade serous ovarian cancer. Nestin may also be considered a novel therapeutic target for antiangiogenic agents.

Cited by 10 publications

References 22 publications

Plxdc family members are novel receptors for the rhesus monkey rhadinovirus (RRV)

Plxdc family members are novel receptors for the rhesus monkey rhadinovirus (RRV)

Cancer stem cells markers in ovarian cancer: Clinical and therapeutic significance (Review)

Plxdc family members are novel receptors for the rhesus monkey rhadinovirus (RRV)

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