Prognostic Significance of Wnt-1, β-catenin and E-cadherin Expression in Advanced Colorectal Carcinoma

Stańczak, Aleksandra; Stec, Rafał; Bodnar, Lubomir; Olszewski, Wojciech; Cichowicz, Marzena; Kozłowski, Wojciech; Szczylik, Cezary; Pietrucha, Tadeusz; Wieczorek, Maciej; Lamparska‐Przybysz, Monika

doi:10.1007/s12253-011-9409-4

Cited by 73 publications

(51 citation statements)

References 44 publications

(58 reference statements)

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“…the results go withHashimoto [19],who observed nuclear expression in 48% of the cancer. Also similar toStanczak [32] who detected unusual cytoplasmic and nuclear β-catenin in 51.5% (34/66) and 31.8% (21/66) of patients, respectively…”

Section: Materials and Methods:-supporting

confidence: 82%

Clinicopathological Significance of Fascin1 and Β-Catenin Expression in Colorectal Adenocarcinoma; An Immunohistochemical Study"

Atwa¹,

Abdelbary.²,

Baiomy³

et al. 2017

IJAR

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Section: Materials and Methods:-supporting

confidence: 82%

Clinicopathological Significance of Fascin1 and Β-Catenin Expression in Colorectal Adenocarcinoma; An Immunohistochemical Study"

Atwa¹,

Abdelbary.²,

Baiomy³

et al. 2017

IJAR

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“…Even the current literature shows conflicting results regarding the expression profiles of Wnt-1 in CRC. For example, Stanczak et al (15) determined the expression and localization of E-cadherin, β-catenin and Wnt-1 proteins in advanced CRC immunohistochemically. They demonstrated a strong Wnt-1 staining in the normal colonic epithelium, while in the majority of primary tumors the expression was decreased.…”

Section: Discussionmentioning

confidence: 99%

Expression of wingless-type mouse mammary tumor virus integration site family pathway effectors in lymphatic and hepatic metastases of patients with colorectal cancer: Associations with the primary tumor

et al. 2015

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Abstract. The wingless-type mouse mammary tumor virus integration site family (Wnt) pathway plays a major role in the carcinogenesis of colorectal cancer (CRC). Its most important effector, the nuclear β-catenin, influences not only transcription but also the proliferation and dedifferentiation of the colonic mucosa. This induces an epithelial-mesenchymal transition which ultimately can lead to the development of cancer and the formation of metastases. However, little is known about the exact interaction and context-sensitive expression of Wnt-pathway effectors in the primary tumor and corresponding metastasis. Therefore, this study assessed the expression of the three most important effectors of the Wnt pathway, β-catenin, adenomatous polyposis coli (APC) and Wnt-1, in the primary tumor and corresponding metastasis of patients with CRC. Immunohistochemical staining of β-catenin, APC and Wnt-1 was performed in paraffin-embedded tissue samples of the primary tumor, and the corresponding hepatic and nodal metastasis samples from 24 patients with metastatic CRC. Isotype antibodies were used as negative controls. The results were visualized using the ABC-method. Analysis of the primary tumor comprised of a separate evaluation of the central tumor area as well as the invasion front. There was a significant overexpression of nuclear β-catenin at the tumor invasion front (P<0.001). Compared to normal colonic mucosa, expression of cytoplasmic β-catenin was significantly higher in the primary tumor (P<0.001) as well as all the corresponding hepatic and lymphatic metastases (hepatic metastases, P=0.001; nodal metastases, P=0.017). By contrast, APC expression was significantly lower in all analyzed tumor compartments compared with normal colonic mucosa (primary tumor, P=0.022; hepatic metastases, P=0.006; nodal metastases, P=0.012). Finally, Wnt-1 protein expression was significantly lower in liver metastases but not in the primary tumor or lymphatic metastases compared with normal colonic mucosa (P=0.003). The present study demonstrates that the major Wnt-effector proteins, β-catenin, APC and Wnt-1, are heterogeneously expressed in the primary tumor and corresponding hepatic as well as nodal metastases of patients with CRC. This context-sensitive diverse expression of Wnt-effector proteins may be important for future individualized targeted therapies.

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“…Mutant APC disrupts the formation of the destruction complex leading to stabilization and accumulation of β-catenin protein in the cytoplasm. Accumulated β-catenin protein is translocated to the cell nucleus where it forms complexes with TCF/LEF, and induces overactivation of Wnt downstream effectors that, in turn, promote the proliferation, migration, invasion and metastasis of cancerous cells (57). The same outcome is also observed with mutations in β-catenin (58) and AXIN2 (57), but to a lesser extent.…”

Section: Molecular Basis Of Crcmentioning

confidence: 94%

The molecular characteristics of colorectal cancer: Implications for diagnosis and therapy (Review)

2018

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Abstract. Colorectal cancer (CRC) results from the progressive accumulation of multiple genetic and epigenetic aberrations within cells. The progression from colorectal adenoma to carcinoma is caused by three major pathways: Microsatellite instability, chromosomal instability and CpG island methylator phenotype. A growing body of scientific evidences suggests that CRC is a heterogeneous disease, and genetic characteristics of the tumors determine their prognostic outcome and response to targeted therapies. Early diagnosis and effective targeted therapies based on a current knowledge of the molecular characteristics of CRC are essential to the successful treatment of CRC. Therefore, the present review summarized the current understanding of the molecular characteristics of CRC, and discussed its implications for diagnosis and targeted therapy.

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Prognostic Significance of Wnt-1, β-catenin and E-cadherin Expression in Advanced Colorectal Carcinoma

Cited by 73 publications

References 44 publications

Clinicopathological Significance of Fascin1 and Β-Catenin Expression in Colorectal Adenocarcinoma; An Immunohistochemical Study"

Clinicopathological Significance of Fascin1 and Β-Catenin Expression in Colorectal Adenocarcinoma; An Immunohistochemical Study"

Expression of wingless-type mouse mammary tumor virus integration site family pathway effectors in lymphatic and hepatic metastases of patients with colorectal cancer: Associations with the primary tumor

The molecular characteristics of colorectal cancer: Implications for diagnosis and therapy (Review)

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