Prognostic Utility of LncRNAs (LINC00565 and LINC00641) as Molecular Markers in Glioblastoma Multiforme (GBM)

Abstract

Background and aim. Glioblastoma multiforme (GBM) are primary brain tumor grade IV characterized by fast cell proliferation, high mortality and morbidity and most lethal gliomas. Molecular approaches underlying its pathogenesis and progression with diagnostic and prognostic value have been an area of interest. Long-non coding RNA (lncRNA) aberrantly expressed in GBM have been recently studied. The aim is to investigate the clinical role of lncRNA565 and lncRNA641 in GBM patients. Patients and methods. Blood samples were withdrawn from 35 newly diagnosed GBM cases with 15 healthy individuals, then lncRNA565 and lncRNA641 expression were evaluated using real time-PCR.Their diagnostic efficacy was detected using receiver operating characteristic curve. Progression free survival (PFS) and overall survival (OS) were studied using Kaplan-Meier curves. Results. LncRNAs expression was increased significantly among GBM as compared to control group. Their expression was correlated with clinico-pathological data and survival pattern for the studied GBM patients. Higher levels of both Lnc RNAs were correlated to worse performance status. Expression of lncRNA565 was increased with large tumor size (≥ 5cm).Survival analysis showed that both investigated lncRNA were increased with worse PFS and OS. Conclusion. Expression of lncRNA 565 and lncRNA 641 in a liquid biopsy sample can be used as prognostic biomarker for GBM patients.