PROGNOSTIC VALUE OF CYTOMEGALOVIRUS IgM ANTIBODY IN TRANSPLANT RECIPIENTS

Wreghitt, T.G.; Gray, Jim; Chandler, C.

doi:10.1016/s0140-6736(86)91874-x

Cited by 14 publications

(4 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All patients with CMV mononucleosis also had raised CMV IgM-, IgE-, IgA-, and IgG-class antibodies, but the decline in response was rapid, with some having a negative or borderline-positive response 2 to 3 months after the onset of symptoms. These observations correspond well with previous findings with indirect ELISAs, RIA, and IFT (4,9,17,20,(24)(25)(26)29). We also found that half the patients with a secondary posttransplant CMV infection had raised specific IgM antibodies, but some of these patients may also produce CMV antibodies of the IgE class ( Fig.…”

Section: Discussionsupporting

confidence: 92%

Kinetics of specific immunoglobulins M, E, A, and G in congenital, primary, and secondary cytomegalovirus infection studied by antibody-capture enzyme-linked immunosorbent assay

1988

View full text Add to dashboard Cite

Antibody-capture enzyme-linked immunosorbent assay (ELISA) using enzyme-labeled cytomegalovirus (CMV) nuclear antigen is a reliable and easily performed test suitable for routine use. As the serologic response to CMV infection may, however, vary considerably among patients, we have studied the kinetics of CMV-specific immunoglobulin M (IgM), IgE, IgA, and IgG antibodies in 352 sera from 61 patients by using antibody-capture ELISA and complement fixation (CF) tests. In a CMV mononucleosis group (n = 17), most patients had antibodies of all four immunoglobulin classes, but antibody levels decreased rapidly, with half the patients having a borderline-positive or a negative reaction for all classes, except IgG, 2 months after the appearance of symptoms. Twelve patients with a primary CMV infection after renal or bone marrow transplantation also developed all immunoglobulin-class antibodies. In only two patients did CMV IgM and IgE antibodies precede seroconversion of CF antibodies, and in one patient, these antibodies lagged months behind. Most patients had all classes of CMV antibodies, except IgA, for a year or more. Among 10 transplant patients with a secondary CMV infection, 50% had long-lasting IgM antibodies, and very few had IgE or IgA antibodies, but all had IgG antibodies to CMV. In 13 infected infants, the CMV-specific serologic response was also characterized by long-lasting IgM, IgE, and IgG antibodies. Two patients did not develop detectable IgM antibodies, and one of these did not show IgE antibodies either. The IgA response in infants as a whole was * Corresponding author.

show abstract

Section: Discussionsupporting

confidence: 92%

Kinetics of specific immunoglobulins M, E, A, and G in congenital, primary, and secondary cytomegalovirus infection studied by antibody-capture enzyme-linked immunosorbent assay

1988

View full text Add to dashboard Cite

show abstract

“…CMV infection can be assessed serologically by using the complement fixation test (CFT), competitive enzyme-linked immunosorbent assay (ELISA) and the detection of specific antibody to CMV (IgM and IgG) [145,150]. In patients free of detectable CMV antibody before transplantation, primary CMV infection is diagnosed when a significant rise in CMV antibody is demonstrated in blood samples taken after transplantation (~four-fold rise by CFT, >50% inhibition in competitive ELISA) [142][143][144][145].…”

Section: Cytomegalovirus Infectionsmentioning

confidence: 99%

Lung allograft transplantation: indications, preoperative assessment and postoperative management

Héritier¹,

Madden²,

M³

et al. 1992

Eur Respir J

View full text Add to dashboard Cite

In spite of a shortage of available donors, an increasing number of heart-lung transplantations have been performed within the last decade. This procedure, first limited to patients with pulmonary vascular disease, has been successfully extended to patients with end stage lung disease, including cystic fibrosis. More recently, single lung, double-lung and bilateral single lung transplantation have become other therapeutic options. Better selection of patients and donors as well as improvements in surgical techniques and immunosuppression regimens have contributed to the reduction in the high perioperative mortality experienced in the early stages. Moreover, the introduction of daily spirometry and transbronchial lung biopsies have permitted early and reliable diagnosis of opportunistic infection and rejection. The most serious late complication of lung transplantation is obliterative bronchiolitis and further research is urgently required to improve diagnosis and management of this condition.

show abstract

“…Detection of CMV IgM antibodies in pregnant women can, therefore, help alert physicians to the potential risk of congenital CMV infection . In addition to pregnant women, transplantation recipients are also at risk of opportunistic infectious diseases associated with CMV infection; thus, detection of CMV IgM in their plasma is important for evaluating their prognosis . CMV IgM can be detected by IB, RIA, IFA, and ELISA.…”

Section: Introductionmentioning

confidence: 99%

“…5,6 In addition to pregnant women, transplantation recipients are also at risk of opportunistic infectious diseases associated with CMV infection; 7 thus, detection of CMV IgM in their plasma is important for evaluating their prognosis. [8][9][10][11] CMV IgM can be detected by IB, RIA, IFA, and ELISA. IFA is the least sensitive of these assays; the others have similar sensitivities.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the indirect and IgM‐capture anti‐human cytomegalovirus IgM ELISA methods as confirmed by cytomegalovirus IgG avidity

Ikuta

Koshizuka

Kanno

et al. 2019

Microbiology and Immunology

View full text Add to dashboard Cite

Primary cytomegalovirus (CMV) infection during pregnancy often results in congenital CMV infection with severe clinical complications. IgM antibodies are one of the indices of primary infection. The IgG avidity index (AI) is also known to remain low for 3 months after primary infection. Here, we evaluated and compared the performance of CMV IgM and IgG avidity assays. Because sensitivity and specificity reportedly differ between CMV IgM kits, CMV IgM detection was compared between the two commercially available ELISA kits that are most commonly used in Japan. Sera for CMV IgM were first screened using a traditional indirect ELISA kit. Selected samples were then tested for CMV IgM and CMV AI using a CMV IgM‐capture ELISA kit and a CMV IgG avidity assay, respectively. The rate of concordance between the IgM kits was 89% (42/47), indicating the absence of any significant difference. Most of the CMV IgM‐positive plasma samples showed high CMV IgG AI; however, 18 commercially available plasma samples with low CMV IgG AI were all CMV IgM‐positive. One plausible explanation for this discrepancy is that the duration of low IgG AI is shorter than that of IgM positivity. Alternatively, CMV IgM tests may generate pseudo‐positive readouts in cases of congenital infection. Nevertheless, our study confirms that CMV IgG AI can be a reliable indicator of CMV primary infection.

show abstract

PROGNOSTIC VALUE OF CYTOMEGALOVIRUS IgM ANTIBODY IN TRANSPLANT RECIPIENTS

Cited by 14 publications

References 4 publications

Kinetics of specific immunoglobulins M, E, A, and G in congenital, primary, and secondary cytomegalovirus infection studied by antibody-capture enzyme-linked immunosorbent assay

Kinetics of specific immunoglobulins M, E, A, and G in congenital, primary, and secondary cytomegalovirus infection studied by antibody-capture enzyme-linked immunosorbent assay

Lung allograft transplantation: indications, preoperative assessment and postoperative management

Evaluation of the indirect and IgM‐capture anti‐human cytomegalovirus IgM ELISA methods as confirmed by cytomegalovirus IgG avidity

Contact Info

Product

Resources

About