Prognostic value of DNA ploidy in 653 Japanese women with node-negative breast cancer

Tsutsui, Shinichi; Ohno, Shinji; Murakami, Shigeru; Hachitanda, Yoichi; Oda, Shinya

doi:10.1007/pl00012103

Cited by 18 publications

(25 citation statements)

References 30 publications

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“…In breast cancer, DNA ploidy and SPF have been investigated in association with the biological characterization of tumours, patient survival and prognosis (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

“…Numerous studies have reported that DNA ploidy and/or SPF are prognostic markers for breast cancer (2)(3)(4)(5)(6)(7)(8)(9)(10)(11); however, a number of studies have questioned their independent prognostic significance (12)(13)(14)(15). These conflicting data have limited the prognostic value of DNA flow cytometry and the extent of its clinical use.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have indicated that the evaluation of cytometric parameters can improve the management of breast cancer, aiding in the prognostic assessment and therapeutic planning for patients (2)(3)(4)(5)(6)9,10,24). The current study aimed to review the application of DNA flow cytometry in the prognostic assessment of patients with breast cancer.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prognostic relevance of DNA flow cytometry in breast cancer revisited: The 25-year experience of the Portuguese Institute of Oncology of Lisbon

et al. 2017

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Abstract. The potential prognostic significance of DNA flow cytometric measurements (DNA ploidy and S-phase fraction) in breast cancer remains in dispute. Inconclusive data, primarily due to the lack of consistent standardization and quality control programs, have limited its translation into clinical practice. The aim of the present review, based on the 25-year experience of the Portuguese Institute of Oncology of Lisbon, is to assess the clinical relevance and application of DNA flow cytometry for the prognosis of breast cancer. Overall, data from Portuguese Institute of Oncology of Lisbon indicate that DNA flow cytometry provides significant prognostic information that is biologically relevant and clinically useful for the management of patients with breast cancer. Furthermore, this data has demonstrated the independent value of DNA aneuploidy as a prognostic indicator of poor clinical outcome in various subgroups of patients with early or locally advanced breast cancer at short-and long-term follow-up. Notably, aneuploidy identifies subsets of patients with grade (G)1 or G2 tumours who exhibit a poor clinical outcome. These patients may benefit from adjuvant chemotherapy, particularly those with luminal A and luminal B/human epidermal growth factor-2-negative endocrine-responsive breast cancer. In conclusion, data from Portuguese Institute of Oncology of Lisbon reinforces the clinical importance and utility of DNA flow cytometric analysis, particularly DNA ploidy, in the prognostic assessment and therapeutic planning for patients with breast cancer.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prognostic relevance of DNA flow cytometry in breast cancer revisited: The 25-year experience of the Portuguese Institute of Oncology of Lisbon

et al. 2017

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“…Measurement of DNA ploidy by static cytometry is correlated with markers of cell differentiation [18]. Many studies have stated that aneuploid tumors are associated with a poorer prognosis than diploid tumors in breast cancer patients with negative lymph nodes [16,19]. Several studies have observed the prognostic value of SPF in breast cancer [20,21].…”

Section: Introductionmentioning

confidence: 99%

“…A number of studies using flow cytometry and static cytometry suggest that the nuclear DNA content has a significant value in the prognosis of breast cancer [13,14,15]. DNA diploid tumors have a more favorable prognosis than those with DNA aneuploid [16]. The DNA aneuploidy is related to markers of poor prognosis such as estrogen and progesterone receptor negativity, positive lymph nodes, large pathological tumor and age younger than 35 years [17].…”

Section: Introductionmentioning

confidence: 99%

Prognostic Significance of DNA Image Cytometry in Libyan Breast Cancer

et al. 2012

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Background: We evaluated the relation of nuclear DNA content and clinicopathological features and prognosis in primary breast cancer of female Libyan patients with variable stage and grade and different treatment regimes. Patients and Methods: Histological samples from 104 patients of breast carcinoma were retrospectively studied by computerized nuclear DNA cytometry. Isolated nuclei from paraffin sections were stained with Feulgen stain and DNA was measured using a computer-assisted image analysis cytometry system. In each case, 200 nuclei were measured and the DNA histograms, S phase fraction (SPF) and number of cells above 5c and 9c were determined. We applied different approaches in the analysis of DNA to compare the DNA histograms with different clinicopathological features and survival. Results: The mean of DNA ploidy mode for all tumors was 3.43; 82.7% of tumors were aneuploid and 17.3% were diploid. The median SPF was 3.5% for DNA diploid and 13.5% for DNA aneuploid tumors. DNA aneuploid tumors and high SPF were associated with advanced stage, distant metastasis, high histological grade and lymph node involvement. The SPF was also associated with large tumor size and with younger patients (<50 years). In the overall population (median follow-up 51 months), patients with aneuploid DNA histograms and high SPF values had shorter survival times than those with diploid DNA histograms and low SPF values (p = 0.001, p < 0.0001, respectively). Also, short survival was associated with a multiploid DNA histogram and with DNA aneuploid cells ≥5 cells (p < 0.0001, p = 0.001, respectively). In a Cox multivariate analysis, DNA ploidy (p = 0.010), age (p = 0.038) and clinical stage (p = 0.001) were independent predictors of overall survival, and DNA ploidy (p = 0.018) and clinical stage (p = 0.001) also proved to be independent predictors of disease-specific survival. The SPF cutoff point of 11% might be applied to separate patients into good and poor prognosis groups. Conclusions: DNA image cytometry with careful analysis of the histograms may provide valuable prognostic information in Libyan breast cancer, with potential clinical implications in patient management, particularly in predicting the patients at high risk for metastasis and recurrence who should be considered as candidates for combined adjuvant therapy.

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Study on Ceramide Expression and DNA Content in Patients With Healthy Mucosa, Leukoplakia, and Carcinoma of the Larynx

Chi

Yuan²,

Wang³

et al. 2004

Arch Otolaryngol Head Neck Surg

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Prognostic value of DNA ploidy in 653 Japanese women with node-negative breast cancer

Abstract: Our findings demonstrated DNA ploidy, based on FCM, to have an important prognostic value in Japanese women with node-negative breast cancer.

Cited by 18 publications

References 30 publications

Prognostic relevance of DNA flow cytometry in breast cancer revisited: The 25-year experience of the Portuguese Institute of Oncology of Lisbon

Prognostic relevance of DNA flow cytometry in breast cancer revisited: The 25-year experience of the Portuguese Institute of Oncology of Lisbon

Prognostic Significance of DNA Image Cytometry in Libyan Breast Cancer

Study on Ceramide Expression and DNA Content in Patients With Healthy Mucosa, Leukoplakia, and Carcinoma of the Larynx

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