Prognostic value of hypoxia-regulated gene expression in loco-regional gastroesophageal cancer

Winther, Mette; Alsner, Jan; Tramm, Trine; Holtved, Eva; Bæksgaard, Lene; Nordsmark, Marianne

doi:10.3109/0284186x.2015.1114680

Cited by 2 publications

(1 citation statement)

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“…Hypoxia is a condition of reduced oxygen concentration that is often present in solid tumors including GA. 8 Besides, hypoxia is one of the common microenvironment features in solid tumors and is relevant to tumor invasiveness, radioinsensitivity and chemo-resistance, as well as increases metastatic potential and poor survival rate. 9 The tumor cells respond to the hypoxic conditions by inducing genes that regulate various biological processes including cellular proliferation, apoptosis, metabolism, angiogenesis and migration. 8 Hypoxia-inducible factors (HIFs) are transcription factors, which are commonly expressed in mammals, including humans.…”

Section: Introductionmentioning

confidence: 99%

<p>HAND2-AS1 Inhibits Gastric Adenocarcinoma Cells Proliferation and Aerobic Glycolysis via miRNAs Sponge</p>

Lv²,

Wang³

et al. 2020

CMAR

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To study the effect of lncRNA HAND2-AS1 on gastric adenocarcinoma (GA) cell property and explore its specific mechanism. Methods: Data on stomach adenocarcinoma (STAD) were analyzed to screen differentially expressed lncRNA HAND2-AS1. RNA22-HAS database and dual luciferase reporter assay were applied to confirm the target relationship between HAND2-AS1/HIF3A and miR-184. The HAND2-AS1 and miR-184 expressions in tissue or cells were determined by qRT-PCR and Western blot. Besides, after GA cells (AGS) cultured in normoxic and hypoxic condition, phosphoenolpyruvate (PEP) and lactic acid were quantified by Phosphoenolpyruvate Fluorometric Assay Kit and Lactic Acid Detection kit, respectively. Additionally, colony formation assay, transwell invasion and migration assays were used to evaluate the abilities of cell invasion, migration, and proliferation in distinct conditions. Results: The HAND2-AS1 and HIF3A expressions were down-regulated and miR-184 expression was up-regulated in GA tissues and cells. Dual luciferase reporter assay confirmed HAND2-AS1 and HIF3A were targeted by miR-184. AGS cell proliferation abilities were restrained by HAND2-AS1 and HIF3A overexpression and enhanced by miR-184, as well as migration and invasion abilities. In addition, HAND2-AS1 rescued enhanced AGS cell proliferation, cell migration, cell invasion abilities and glycolytic process caused by hypoxia via miR-184/HIF3A. Conclusion: LncRNA HAND2-AS1 could inhibit GA cell proliferation, migration and invasion abilities and glycolytic process induced by hypoxia through miR-184/HIF3A signaling.

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Section: Introductionmentioning

confidence: 99%