Prognostic value of spermatological parameters as predictors of in vitro fertility of frozen-thawed bull semen

Tartaglione, C.M.; Ritta, Mónica N.

doi:10.1016/j.theriogenology.2004.01.012

Cited by 130 publications

(96 citation statements)

References 33 publications

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“…It was found earlier that the results of post-thaw hypo-osmotic swelling tests can be used to predict potential fertility of bovine semen samples used for IVF (Tartaglione and Ritta, 2004). According to Brito et al (2003), the HOS test was the only plasmalemma evaluation method that significantly contributed to conventional sperm quality tests in predicting in vitro fertilization rate.…”

Section: Discussionmentioning

confidence: 99%

“…Attempts have previously been made to include the HOS test in the regression equation for fertility. Tartaglione and Ritta (2004) included an eosin-nigrosin supravital stain combined with the HOS test into a regression equation as a predictor of in vitro fertility of FT bull semen. Pérez-Llano et al (2001) also included the HOS test in a regression equation along with other semen parameters such as morphology, motility, and acrosome integrity to predict the fertility of boar semen.…”

Section: Discussionmentioning

confidence: 99%

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Relationships between the results of hypo-osmotic swelling tests, sperm motility, and fertility in Estonian Holstein dairy bulls

Padrik¹,

Hallap²,

Kaart³

et al. 2012

Czech J. Anim. Sci.

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ABSTRACT:As an attempt to find an inexpensive and simple laboratory method for artificial insemination (AI) bull semen quality assessment, the osmotic resistance of spermatozoa was measured using the hypoosmotic swelling (HOS) test, developed by Jeyendran et al. (1984) (labelled HOS-1), and its modifications (HOS-2, HOS-3), with decreased osmotic pressure aimed at challenging sperm survival ability. The test results were benchmarked against sperm viability measurements performed using the Computerized Motility Analyzer (CMA), and field fertility was calculated as non-return rate (NRR). Two age groups of Estonian Holstein bull sires were included in this study to test possible age effect on semen quality parameters. The HOS-1 test in fresh bull semen correlated well with sperm general motility (GMot) (r = 0.63, P < 0.001 at batch level and r = 0.77, P < 0.001 at bull level) as well as with progressive motility (PMot) in frozen-thawed (FT) semen (r = 66, P < 0.001 at batch level and r = 0.81, P < 0.001 at bull level), which makes the test suitable for the prediction of post-thaw semen quality. However, the HOS-2 and HOS-3 values in FT semen had high correlations with NRR (r = 0.65, r = 0.66, P < 0.001 at batch level and r = 0.63, r = 0.71, P < 0.01 at bull level), which was comparable to those between GMot and NRR or PMot and NRR. A combination of motility parameters and the results of the HOS-1 and HOS-3 tests provided a good model for predicting the potential fertility of bull semen. Values of sperm membrane post-thaw intactness, assessed using HOS-2, as well as of sperm motility measurements were higher in mature bulls compared to those in young bulls. Short conclusion: different modifications of the hypo-osmotic swelling test are useful for routine bovine semen quality assessment at AI stations.Keywords: bull fertility; semen quality; sperm membrane intactness; bull age Laboratory analyses have been used over many decades to evaluate semen quality. Despite the fact that none of the single assays developed provide results that consistently and highly correlate with fertility, the laboratory evaluation of semen samples remains an important procedure for the artificial insemination (AI) industry to eliminate low fertility bulls or semen from being used in artificial insemination programmes (Graham and Mocé, 2005).Post-thaw viability and fertility of cryopreserved sperm are often reduced due to accumulated cellular damage during the cryopreservation phases (Muiño et al., 2008). Decrease in temperature, cold shock, and intracellular ice formation can affect sperm plasma membrane, acrosomal and mitochondrial membrane integrity (Thomas et al., 1998;Defoin et al., 2008), and cause the loss of intracellular components (Graham and Mocé, 2005), which can initiate cell death.Sperm membrane integrity can be evaluated by several methods, e.g. light or fluorescent microscopy combined with vital stains (Brito et al., 2003), and flow cytometry (Januskauskas et al.,

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Relationships between the results of hypo-osmotic swelling tests, sperm motility, and fertility in Estonian Holstein dairy bulls

Padrik¹,

Hallap²,

Kaart³

et al. 2012

Czech J. Anim. Sci.

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“…In this sense, many studies have been conducted in order to develop laboratory tests that could predict frozen-thawed semen reproductive performance (TARTAGLIONE;RITTA, 2004). However, classic and modern laboratory tests of semen quality are still unable to accurately estimate real sperm-fertilizing capacity.…”

Section: Introductionmentioning

confidence: 99%

“…Hence, multiple independent variables are often necessary in order to make a reasonably accurate prediction (UTT, 2016). For this reason, it is necessary to associate different semen quality tests so to improve the accuracy of a bull's reproductive potential (JANUSKAUSKAS et al, 2003;TARTAGLIONE;RITTA, 2004;THUNDATHIL, 2008;ARRUDA et al, 2011;OLIVEIRA et al, 2013;SELLEM et al, 2015;CELEGHINI et al, 2017).…”

mentioning

confidence: 99%

Assessment of different in vitro sperm challenges and in vivo fertility of bovine semen batches

Oliveira

Ribeiro

Silva

et al. 2017

Braz. J. Vet. Res. Anim. Sci.

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The aim of this work was to submit sperm cells to different laboratory challenges and to compare in vitro results with in vivo semen fertility. Four different batches from the same Brangus bull were used in a timed-AI program of 332 Brangus cows. Each batch (B) was submitted to the following procedure: semen sample was thawed at 36°C for 30 seconds (control). Sperm motility parameters, plasma membrane integrity, sperm morphology, and concentration were assessed. Then, an aliquot of thawed sample was incubated in a water bath at 45°C for 40 min (thermal challenge group; TCG) and another aliquot was centrifuged at 500 xg (Percoll gradient 45%/90%) for 15 min (centrifugation challenge group; CCG). Centrifuged semen was also submitted to another thermal challenge, being incubated (water bath) at 45°C for 40 min (centrifugation + thermal challenge group; CTCG). At the end of each challenge (CCG, TCG, and CTCG), the same laboratory tests used for control group were repeated. The following conception rates (CR) were observed for each batch: B1 = 48.9% (44/90); B2 = 44.2% (23/52); B3 = 55.5% (40/72); B4 = 43.2% (51/118); (p < 0.10). In the lab, B3 presented higher (p ≤ 0.05) progressive motility (PM) than B4 after thawing (control group) and after all sperm challenges (TCG, CCG, and CTCG). However, despite B3 and B4 having demonstrated a similar percentage of plasma membrane integrity (PMI) to the control group (B3 = 66.7 ± 1.3 and B4 = 65.2 ± 3.3), B3 demonstrated higher (P ≤ 0.05) percentage of PMI (37.2 ± 2.5) than B4 (26.7 ± 3.3) after passing through the most stressing in vitro challenge (CTCG). The semen batch presenting the highest resistance to in vitro challenges was the one that presented a trend for higher in vivo fertility, suggesting that submitting semen samples to laboratory challenges may be an interesting alternative for selecting batches with greater field fertility. Keywords: Conception rate. In vitro sperm resistance. Timed-AI. ResumoO objetivo deste estudo foi estressar células espermáticas em diferentes desafios laboratoriais e comparar os resultados in vitro com a fertilidade in vivo do sêmen. Quatro partidas de um mesmo touro Brangus foram utilizadas em um programa de IATF de 332 vacas Brangus. Cada partida foi submetida ao seguinte procedimento: a amostra de sêmen foi descongelada a 36°C por 30 segundos (grupo controle). Foram avaliados parâmetros de motilidade espermática (CASA), integridade da membrana plasmática (PMI), morfologia e concentração espermática. Em seguida, uma alíquota da amostra descongelada foi incubada em banho-maria a 45°C durante 40 minutos (grupo de desafio térmico, TCG) e outra alíquota foi centrifugada a 500 xg (gradiente de Percoll 45%/90%) durante 15 min (grupo desafio de centrifugação, CCG). Uma aliquota do sêmen centrifugado foi ainda submetida ao desafio térmico, sendo incubado a 45°C durante 40 min (grupo de desafio térmico + centrifugação, CTCG). No final de cada desafio (CCG, TCG e CTCG), os mesmos testes laboratoriais utilizados para o grupo de controle f...

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“…O teste de resistência osmótica foi considerado como um indicador de fertilidade em diversas espécies: humanos (JEYENDRAN et al, 1984;VAN DER VEN et al, 1986;CHAN, et al, 1991;HOSSAIN et al, 1998), eqüinos (NIE et al, 2001;MELO;HENRY, 1999;NEILD et al, 1999), caninos (KUMI-DIAKA, 1993), suínos (PÉREZ-LLANO et al, 2001) e touros (CORREA et al, 1997;ROTA et al, 2000;TARTAGLIONE;RITTA, 2004). Consiste em um método fácil, acessível e barato (CORREA; ZAVOS, 1994;TARTAGLIONE;RITTA, 2004).…”

Section: I T E R a T U R A | 33unclassified

Avaliação das características da motilidade (CASA), morfologia e funcionalidade da membrana plasmática (HOST) de espermatozóides bovinos sexados por cimetria de fluxo

Gallego¹

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A verdade é que, quando sua mente estava completamente alheia, ele tinha o pensamento mais estranho que qualquer lunático no mundo jamais tivera, pois lhe parecia razoável e necessário, tanto por uma questão de honra quanto para servir a nação, se tornar um cavaleiro errante e viajar pelo mundo em sua armadura e seu cavalo para buscar aventuras e envolver-se em tudo que ele havia lido sobre como os cavaleiros errantes se envolviam, aproveitando as oportunidades, colocando-se em perigo e corrigindo todo tipo de mal.-Miguel de Cervantes, Don Quixote. DEDICO.A Dios.A mis Papas Ramiro y Alba Lucia, su amor es la base de todas mis logros, Dios me bendijo al poder tenerlos a mi lado.A mi hermanita María del Pilar, eres la otra faz de una obra de arte, tu alegría y emotividad me llenan de felicidad. No te imaginas como te admiro.A mis abuelitas Ruth y Mary, Las abuelitas más dulces y tiernas que puedan existir, siempre las llevo en mi corazón.A mis Abuelos Libardo y Jorge (In memoriam), Sus vidas son una inspiración para mi. Es un honor llevar el apellido Mejia Gallego. Los Extraño A mi novia Dunia, Porque nuestros sueños se hagan realidad. LOS AMO !!!!! Agradecimientos.A mis tias Angela, Nohora y Liliana: Siempre han creido en mis capacidades, ello me ha llenado de confianza para llegar a donde estoy ahora, de todas he aprendido cosas importantes en la vida.Las llevo y las llevare en mi corazón siempre.A mis tios

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Prognostic value of spermatological parameters as predictors of in vitro fertility of frozen-thawed bull semen

Cited by 130 publications

References 33 publications

Relationships between the results of hypo-osmotic swelling tests, sperm motility, and fertility in Estonian Holstein dairy bulls

Relationships between the results of hypo-osmotic swelling tests, sperm motility, and fertility in Estonian Holstein dairy bulls

Assessment of different in vitro sperm challenges and in vivo fertility of bovine semen batches

Avaliação das características da motilidade (CASA), morfologia e funcionalidade da membrana plasmática (HOST) de espermatozóides bovinos sexados por cimetria de fluxo

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