Prognostic Value of the miR-17~92 Cluster in Chronic Lymphocytic Leukemia

Abstract

The aim of this study was to investigate the expression of miR-17∼92 cluster members in chronic lymphocytic leukemia (CLL) patients. Six microRNAs (miRNAs)—miR-17, miR-18a, miR-19a, miR-19b-1, miR-20a, and miR-92a-1—very poorly characterized in CLL patients, were chosen for the study to consider their possible role as cancer biomarkers. It is currently unclear to which extent miR-17~92 expression is related to other routinely measured CLL markers, and whether the findings can be of any clinical significance. To achieve this goal, we report the expression levels of these miRNAs detected by RT-qPCR in purified CD19+ B lymphocytes of 107 CLL patients and correlate them with existing clinical data. The study provides new evidence regarding the heterogeneity of miR-17~92 cluster members’ expression in CLL patients. Higher miR-17-5p expression was associated with unfavorable prognostic factors (i.e., 17p and 11q deletions, CD38 and ZAP-70 expression). On the other hand, miR-19a, miR-20a, and miR-92a-1 negatively correlated with these adverse factors. The presence of del(13q) as a sole aberration was associated with a significantly lower miR-17-5p as well as higher miR-19a-3p and miR-92a-1-5p expression compared to patients carrying unfavorable genetic aberrations. Particularly, miR-20a could be considered an independent favorable prognostic factor. In a multivariate analysis, high miR-20a expression remained an independent marker predicting long TTT (time to treatment) for CLL patients.

The Screening of microRNAs in Chronic Myeloid Leukemia: A Clinical Evaluation

“…In the first stage, eight microRNAs were evaluated: miR-17-5p [ 10 , 11 , 12 , 13 ], miR-23a-3p [ 14 , 15 , 16 ], miR-93-5p [ 17 , 18 ], miR-130a-3p [ 19 ], miR-142-5p [ 20 , 21 ], miR-148b-3p [ 22 , 23 ], miR-199a-3p [ 24 , 25 ], and miR-331-5p [ 26 ]. These microRNAs have been chosen based on oncology potential found in the literature.…”

Can Galectin-3 Be a Novel Biomarker in Chronic Lymphocytic Leukemia?

“…The Vysis CLL FISH Probe Kit (Abbott GmbH, Wiesbaden, Germany) was used to detect common genomic aberrations: trisomy 12 and deletions of 11q22.3 ( ATM ), 17p13.1 ( TP53 ), and 13q14.3. The FISH protocol was described previously [ 26 ].…”

Диагностический Потенциал Регуляторных Не Кодирующих Белок РНК При Хроническом Лимфоцитарном Лейкозе

“…Однако их транскрипция значительно увеличивается при немутантном варианте ХЛЛ по генам IgHV. Показано, что высокая экспрессия miR-17-5p связана с другими неблагоприятными прогностическими факторами (в т. ч. экспрессией CD38 и ZAP-70), а miR-20a можно, наоборот, рассматривать как благоприятный прогностический фактор, т. к. она тесно связана с низкой экспрессией ZAP-70/CD38 и с отсутствием неблагоприятных цитогенетических аберраций [35].…”