Prognostic values and prospective pathway signaling of MicroRNA-182 in ovarian cancer: a study based on gene expression omnibus (GEO) and bioinformatics analysis

Li, Yaowei; Li, Li

doi:10.1186/s13048-019-0580-7

Cited by 26 publications

(14 citation statements)

References 59 publications

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“…MiRNA-182-5p is a new emerging miRNA and has been confirmed to be involved in several diseases [23, [39][40][41][42]. In this study, miRNA-182-5p was found to be up-regulated significantly in colitis model group, which indicates an underlying role of it in colitis.…”

Section: Discussionsupporting

confidence: 54%

MiRNA-182-5p aggravates experimental ulcerative colitis via sponging Claudin-2

et al. 2021

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Tight junction proteins play crucial roles in maintaining the integrity of intestinal mucosal barrier. MiRNA-182-5p is capable of targeting claudin-2 which is one of the vital tight junction proteins and the effect and mechanism of miRNA-182-5p was explored here in the DSS-induced colitis model. The pathological conditions were evaluated via hematoxylin and eosin staining. The gene expression level was assessed via PCR. Quantitative immunohistochemistry analysis was performed for the measurement of claudin-2. microRNA.org online tool was used for target gene prediction. Luciferase reporter assay and RNA pull-down assay were performed to detect the target of miRNA-182-5p. The inflammatory and oxidative stress level were measured using corresponding kits. MiRNA-182-5p was highly expressed in colitis model and miRNA-182-5p inhibitor exerted protective effects on colitis induced by DSS in mice. The protective effects includded improvement of pathological changes, increases in anti-inflammation and anti-oxidative genes, and up-regulation of TGF-β1. Claudin-2 mRNA was predicted as the target of miRNA-182-5p, which was validated via luciferase reporter assay and RNA pull-down assay. Claudin-2 overexpression was found in miRNA-182-5p inhibitor group. Consistent with the role of miRNA-182-5p, claudin-2 overexpression also exerted protective effects on DSS-induced colitis in mice. Inhibition of miRNA-182-5p exerted protective effects on colitis via targeting and upregulating claudin-2. The findings in study provide a new therapeutic strategy for colitis treatment and lay the foundation for future study.

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Section: Discussionsupporting

confidence: 54%

MiRNA-182-5p aggravates experimental ulcerative colitis via sponging Claudin-2

et al. 2021

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“…Most importantly, this study focused on the targeting relationship between miR-182-5p and STARD13, thereby improving regulatory mechanism of miR-182-5p in LUAD at the molecular level. Several studies reported the role of miR-182-5p in varying cancer progression [21,22]. For instance, miR-182-5p facilitates cell viability, mitosis, migration, and invasion in human gastric cancer through RAB27A downregulation [23].…”

Section: Discussionmentioning

confidence: 99%

miR-182-5p Serves as an Oncogene in Lung Adenocarcinoma through Binding to STARD13

Wang

et al. 2021

Computational and Mathematical Methods in Medicine

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Lung cancer as one of the commonest invasive malignancies is featured by high morbidity and mortality, wherein lung adenocarcinoma (LUAD) is the most prevalent subtype. Accumulating evidence exhibited that microRNAs are involved in LUAD occurrence and progression. In this study, miR-182-5p was observed to increase in both LUAD tissue and cell lines. Overexpression of miR-182-5p could prominently facilitate cell proliferation, migration, and invasion in LUAD. Through bioinformatics analysis, STARD13 was theorized as the target gene of miR-182-5p, which was lowly expressed in LUAD. Further molecular experiments manifested that miR-182-5p bound to the 3 ′ -untranslated region of STARD13, and there was an inverse correlation between STARD13 and miR-182-5p in LUAD. Rescue experiments demonstrated that silencing STARD13 conspicuously restored the inhibitory effect of decreased miR-182-5p on cell proliferation, migration, and invasion in LUAD. Together, our findings revealed novel roles of the miR-182-5p/STARD13 axis in LUAD progression.

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“…DCN (Decorin), AKT3 (AKT Serine/Threonine Kinase 3), and TIMP2 (tissue inhibitor of metalloproteinases 2) are the other novel candidate target genes for miR-182 involved in the regulation of ovarian cancer biological processes [ 173 ]. Due to the above statements, blocking the expression of miR-182 will rescue some well-known genes suppressing the tumor and recover the cell's critical biologic functions.…”

Section: Mirnas and The Pathogenesis And Metastasis Of Ovarian Cancermentioning

confidence: 99%

MicroRNAs as Biomarkers for Early Diagnosis, Prognosis, and Therapeutic Targeting of Ovarian Cancer

Mirahmadi

Nabavi

Taheri

et al. 2021

Journal of Oncology

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Ovarian cancer is the major cause of gynecologic cancer-related mortality. Regardless of outstanding advances, which have been made for improving the prognosis, diagnosis, and treatment of ovarian cancer, the majority of the patients will die of the disease. Late-stage diagnosis and the occurrence of recurrent cancer after treatment are the most important causes of the high mortality rate observed in ovarian cancer patients. Unraveling the molecular mechanisms involved in the pathogenesis of ovarian cancer may help find new biomarkers and therapeutic targets for ovarian cancer. MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression, mostly at the posttranscriptional stage, through binding to mRNA targets and inducing translational repression or degradation of target via the RNA-induced silencing complex. Over the last two decades, the role of miRNAs in the pathogenesis of various human cancers, including ovarian cancer, has been documented in multiple studies. Consequently, these small RNAs could be considered as reliable markers for prognosis and early diagnosis. Furthermore, given the function of miRNAs in various cellular pathways, including cell survival and differentiation, targeting miRNAs could be an interesting approach for the treatment of human cancers. Here, we review our current understanding of the most updated role of the important dysregulation of miRNAs and their roles in the progression and metastasis of ovarian cancer. Furthermore, we meticulously discuss the significance of miRNAs as prognostic and diagnostic markers. Lastly, we mention the opportunities and the efforts made for targeting ovarian cancer through inhibition and/or stimulation of the miRNAs.

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Prognostic values and prospective pathway signaling of MicroRNA-182 in ovarian cancer: a study based on gene expression omnibus (GEO) and bioinformatics analysis

Cited by 26 publications

References 59 publications

MiRNA-182-5p aggravates experimental ulcerative colitis via sponging Claudin-2

MiRNA-182-5p aggravates experimental ulcerative colitis via sponging Claudin-2

miR-182-5p Serves as an Oncogene in Lung Adenocarcinoma through Binding to STARD13

MicroRNAs as Biomarkers for Early Diagnosis, Prognosis, and Therapeutic Targeting of Ovarian Cancer

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