Prognostication of Vulvar Cancer Based on p14ARF Status: Molecular Assessment of Transcript and Protein

Lavorato-Rocha, André Mourão; Maia, Beatriz de Melo; Rodrigues, Iara S.; Stiepcich, Monica Maria; Baiocchi, Glauco; Cestari, Flávia Munhoz Da Silva; Carvalho, Kátia Cândido; Soares, Fernando Augusto

doi:10.1245/s10434-012-2560-7

Cited by 18 publications

(13 citation statements)

References 41 publications

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“…These results, together with other results from our group, drive us to conclude that HPV-positive tumours do not show evidences of EMT-like events, with usually better prognosis (De Melo Maia et al , 2012; Lavorato-Rocha et al 2013). On the other hand, the HPV-negative tumours develop EMT-like and, therefore increased capability of invasion and progression, leading to worse prognosis and poorer outcome.…”

Section: Discussionsupporting

confidence: 79%

Epithelial-mesenchymal transition-like events in vulvar cancer and its relation with HPV

et al. 2013

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Background:Epithelial-to-mesenchymal transition (EMT) still remains an obscure event in vulvar squamous cell carcinoma (VSCC).Methods:Immunohistochemistry (IHC) expression of E-cadherin, β-catenin, Snail, Slug, Twist and Vimentin was analysed in 87 VSCC, controlled for human papillomavirus (HPV) positivity, considering tumour front and central tumour as different morphological categories from the same tumour.Results:Lower β-catenin and higher Vimentin expression was associated with invasive front when compared with the central tumour (P=0.013 and P⩽0.001, respectively). Higher expression of E-cadherin in central tumour was significantly related to absence of vascular and perineural invasion, lower invasion depth and ⩽2 lymph node involvement. Loss of β-catenin and high Slug, Snail and Twist expression was associated with HPV-negative tumours. Moreover, β-catenin lower expression associated with gain in Slug expression predicts a subgroup with worst outcome (P=0.001). Lower expression of β-catenin in both central tumour and invasive front correlated with lower overall survival (P=0.021 and P=0.011, respectively). Also, multivariate analysis showed that lower β-catenin expression was independently associated with poorer outcome (P=0.044).Conclusion:Human papillomavirus-related tumours show better prognosis and outcome; besides, they do not progress through EMT phenomenon. Immunohistochemical analysis of β-catenin in invasive tumour front is a key issue for establishing prognosis of vulva cancer.

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Section: Discussionsupporting

confidence: 79%

Epithelial-mesenchymal transition-like events in vulvar cancer and its relation with HPV

et al. 2013

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“…The majority of studies ( n = 15) used formalin‐fixed paraffin‐embedded tissue samples for diagnosis and HPV testing. Most studies ( n = 14) used polymerase chain reaction (PCR)‐based test to detect HPV DNA, while two studies used in situ hybridization (ISH) and one study used hybrid capture 2 (HC2). Finally, one study used p16 immunohistochemistry (p16 IHC) as a surrogate marker for HPV infection …”

Section: Resultsmentioning

confidence: 99%

Does HPV status influence survival after vulvar cancer?

Rasmussen

Sand

Frederiksen

et al. 2017

Intl Journal of Cancer

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High-risk human papillomavirus (HPV) infection is essential in the carcinogenesis of a substantial part of anogenital and oropharyngeal cancers and has additionally been shown to be a possible predictive marker for survival, especially in oropharyngeal cancer. Studies examining the influence of HPV status on survival after vulvar cancer have been conflicting and limited by small study populations. Therefore, the aim of this review and meta-analysis was to examine whether HPV status influences survival after vulvar cancer, which, to our knowledge, has not been done before. We conducted a systematic search of PubMed, Cochrane Library and Embase to identify studies examining survival after histologically verified and HPV tested vulvar cancer. A total of 18 studies were eligible for inclusion. Study-specific and pooled HRs of the 5-year OS and DFS were calculated using a fixed effects model. The I statistic was used to describe heterogeneity. The studies included a total of 1,638 women with HPV tested vulvar cancers of which 541 and 1,097 were HPV-positive and HPV-negative, respectively. Fifteen studies included only squamous cell carcinomas. We found a pooled HR of 0.61 (95% CI: 0.48-0.77) and 0.75 (95% CI: 0.57-1.00) for 5-year OS and DFS, respectively. Across study heterogeneity was moderate to high (OS: I = 51%; DFS: I = 73%). In conclusion, women with HPV-positive vulvar cancers have a superior survival compared to women with HPV-negative, which could be of great clinical interest and provides insight into the differences in the natural history of HPV-positive and negative vulvar cancers.

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“…Despite the discovery of potential prognostic risk factors [16,17] LN involvement is still the most important prognostic factor in vulva carcinoma [1,[4][5][6]. Moreover, it is suggested that the amount of removed LNs is associated with GR rates.…”

Section: Discussionmentioning

confidence: 99%

The number of removed lymph nodes by inguinofemoral lymphadenectomy: impact on recurrence rates in patients with vulva carcinoma

Diehl

Volland

Kirn

et al. 2015

Arch Gynecol Obstet

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Prognostication of Vulvar Cancer Based on p14ARF Status: Molecular Assessment of Transcript and Protein

Cited by 18 publications

References 41 publications

Epithelial-mesenchymal transition-like events in vulvar cancer and its relation with HPV

Epithelial-mesenchymal transition-like events in vulvar cancer and its relation with HPV

Does HPV status influence survival after vulvar cancer?

The number of removed lymph nodes by inguinofemoral lymphadenectomy: impact on recurrence rates in patients with vulva carcinoma

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