Programed Assembly of Nucleoprotein Nanoparticles Using DNA and Zinc Fingers for Targeted Protein Delivery

Ryu, Yiseul; Kang, Jung Ae; Kim, Dasom; Kim, Song Rae; Kim, Seungmin; Park, Seong Ji; Kwon, Soon-Jung; Kim, Kil Nam; Lee, Dong‐Eun; Lee, Joong jae; Kim, Kwang Ho

doi:10.1002/smll.201802618

Cited by 12 publications

(9 citation statements)

References 59 publications

(84 reference statements)

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“…The homeobox TF regulates the expression of genes associated with various developmental processes in animals, fungi and plants [40]. The zf-C2H2 TF family contains a small protein structural motif, the zinc finger (zf ), which coordinates one or more zinc ions (Zn 2 + ) [41]. TFs containing zinc fingers have been implicated in a variety of biological processes in T. gondii [42,43].…”

Section: Discussionmentioning

confidence: 99%

Transcriptional changes in Toxoplasma gondii in response to treatment with monensin

Zhai

Elsheikha

et al. 2020

Parasites Vectors

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Background: Infection with the apicomplexan protozoan parasite T. gondii can cause severe and potentially fatal cerebral and ocular disease, especially in immunocompromised individuals. The anticoccidial ionophore drug monensin has been shown to have anti-Toxoplasma gondii properties. However, the comprehensive molecular mechanisms that underlie the effect of monensin on T. gondii are still largely unknown. We hypothesized that analysis of T. gondii transcriptional changes induced by monensin treatment can reveal new aspects of the mechanism of action of monensin against T. gondii.Methods: Porcine kidney (PK)-15 cells were infected with tachyzoites of T. gondii RH strain. Three hours post-infection, PK-15 cells were treated with 0.1 μM monensin, while control cells were treated with medium only. PK-15 cells containing intracellular tachyzoites were harvested at 6 and 24 h post-treatment, and the transcriptomic profiles of T. gondii-infected PK-15 cells were examined using high-throughput RNA sequencing (RNA-seq). Quantitative real-time PCR was used to verify the expression of 15 differentially expressed genes (DEGs) identified by RNA-seq analysis.Results: A total of 4868 downregulated genes and three upregulated genes were identified in monensin-treated T. gondii, indicating that most of T. gondii genes were suppressed by monensin. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of T. gondii DEGs showed that T. gondii metabolic and cellular pathways were significantly downregulated. Spliceosome, ribosome, and protein processing in endoplasmic reticulum were the top three most significantly enriched pathways out of the 30 highly enriched pathways detected in T. gondii. This result suggests that monensin, via down-regulation of protein biosynthesis in T. gondii, can limit the parasite growth and proliferation. Conclusions:Our findings provide a comprehensive insight into T. gondii genes and pathways with altered expression following monensin treatment. These data can be further explored to achieve better understanding of the specific mechanism of action of monensin against T. gondii.

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Section: Discussionmentioning

confidence: 99%

Transcriptional changes in Toxoplasma gondii in response to treatment with monensin

Zhai

Elsheikha

et al. 2020

Parasites Vectors

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“…By contrast, proteins that exhibit a DNA binding affinity in a sequence-dependent or independent mode include cationic peptides [ 25 , 26 , 27 , 28 ], cationic polymer proteins [ 29 , 30 , 31 ], ribosomal proteins [ 32 ], transcription activator-like (TAL) effectors [ 17 ], transcription factors [ 33 ], viral proteins [ 34 , 35 , 36 ], histones, and polymerases [ 37 ]. Simpler options such as streptavidin or bioinspired cationic protein polymers made up of extremely simple and repetitive amino acids that retain DNA-binding functionality or even virus-like properties have also been used [ 7 , 25 , 38 ]. These proteins can be used in combination with junctions, tiles, motifs, or origamis, and also single ssDNA or dsDNA molecules.…”

Section: Proteins In Hybrid Nanotechnologymentioning

confidence: 99%

Strategies to Build Hybrid Protein–DNA Nanostructures

Hernández-García

2021

Nanomaterials

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Proteins and DNA exhibit key physical chemical properties that make them advantageous for building nanostructures with outstanding features. Both DNA and protein nanotechnology have growth notably and proved to be fertile disciplines. The combination of both types of nanotechnologies is helpful to overcome the individual weaknesses and limitations of each one, paving the way for the continuing diversification of structural nanotechnologies. Recent studies have implemented a synergistic combination of both biomolecules to assemble unique and sophisticate protein–DNA nanostructures. These hybrid nanostructures are highly programmable and display remarkable features that create new opportunities to build on the nanoscale. This review focuses on the strategies deployed to create hybrid protein–DNA nanostructures. Here, we discuss strategies such as polymerization, spatial directing and organizing, coating, and rigidizing or folding DNA into particular shapes or moving parts. The enrichment of structural DNA nanotechnology by incorporating protein nanotechnology has been clearly demonstrated and still shows a large potential to create useful and advanced materials with cell-like properties or dynamic systems. It can be expected that structural protein–DNA nanotechnology will open new avenues in the fabrication of nanoassemblies with unique functional applications and enrich the toolbox of bionanotechnology.

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“…By contrast, proteins that exhibit a DNA binding affinity in a sequence-dependent or independent mode include cationic peptides [25][26][27][28], cationic polymer proteins [29][30][31], ribosomal proteins [32], transcription activator-like (TAL) effectors [17], transcription factors [33], viral proteins [34][35][36], histones, and polymerases [37]. Simpler options such as streptavidin or bioinspired cationic protein polymers made up of extremely simple and repetitive amino acids that retain DNAbinding functionality or even virus-like properties have also been used [7,25,38]. These proteins can be used in combination with junctions, tiles, motifs, or origamis, and also single ssDNA or dsDNA molecules.…”

Section: Proteins In Hybrid Nanotechnologymentioning

confidence: 99%

Strategies to Build Hybrid Protein-DNA Nanostructures

Hernández-García¹

2021

Preprint

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Proteins and DNA exhibit key physical chemical properties that make them advantageous for building nanostructures with outstanding features. Both DNA and protein nanotechnology have growth notably and proved to be fertile disciplines. The combination of both types of nanotechnologies is helpful to overcome the individual weaknesses and limitations of each one, paving the way for the continuing diversification of the structural nanotechnologies. Recent studies have implemented a synergistic combination of both biomolecules to assemble unique and sophisticate protein-DNA nanostructures. These hybrid nanostructures are highly programmable and display remarkable features that create new opportunities to build in the nanoscale. This review focuses on the strategies deployed to create hybrid protein-DNA nanostructures. Here, we will discuss strategies such as polymerization, spatial directing and organizing, coating, rigidizing or folding DNA into particular shapes or moving parts. The enrichment of structural DNA nanotechnology by incorporating protein nanotechnology has been clearly demonstrated and still shows a large potential to create useful and advanced materials with cell-like properties or dynamic systems. It can be expected that structural protein-DNA nanotechnology will open new avenues in the fabrication of nano-assemblies with unique functional applications and enrich the toolbox of bionanotechnology.

show abstract

Programed Assembly of Nucleoprotein Nanoparticles Using DNA and Zinc Fingers for Targeted Protein Delivery

Cited by 12 publications

References 59 publications

Transcriptional changes in Toxoplasma gondii in response to treatment with monensin

Transcriptional changes in Toxoplasma gondii in response to treatment with monensin

Strategies to Build Hybrid Protein–DNA Nanostructures

Strategies to Build Hybrid Protein-DNA Nanostructures

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