2019
DOI: 10.1101/846055
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Programmable CRISPR interference for gene silencing using Cas13a in mosquitoes

Abstract: In the CRISPR-Cas systems, Cas13a is an RNA-guided RNA nuclease specifically targeting single strand RNA. In this study we developed a Cas13a mediated CRISPR interference tool to target mRNA for gene silencing in mosquitoes and tested the machinery in two mosquito species. A Cas13a expressing plasmid was delivered to mosquitoes by intrathoracic injection, and Cas13a transcripts were detectable at least 10 days post-delivery. In Anopheles gambiae, vitellogenin (Vg) gene was silenced by Vg-crRNA injection two ho… Show more

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