Programmable DNA Nanosystem for Molecular Interrogation

Mathur, Divita; Henderson, Eric

doi:10.1038/srep27413

Cited by 15 publications

(18 citation statements)

References 41 publications

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“…A growing repository of DNA nanostructures are now available that show dynamic actuation and reconfigurability in response to external stimuli; this is also what helps distinguish the potential of this class of technology from most other sensor types. [14a,46,116] Reconfigurability enables designing distinct positive and negative states (target present or absent) in DNA‐based sensors thereby reducing signal noise commonly found in existing sensing techniques. For instance, electrochemical biosensors, wherein analyte detection is transduced into an electrical signal, when integrated with DNA structures showed enhanced signal to noise and, in some cases, amplified the signal since the probe was physically much closer to the sensing electrode .…”

Section: Dna Nanostructures As Biomedical Sensorsmentioning

confidence: 99%

The Growing Development of DNA Nanostructures for Potential Healthcare‐Related Applications

Mathur

Medintz

2019

Adv Healthcare Materials

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DNA self‐assembly has proven to be a highly versatile tool for engineering complex and dynamic biocompatible nanostructures from the bottom up with a wide range of potential bioapplications currently being pursued. Primary among these is healthcare, with the goal of developing diagnostic, imaging, and drug delivery devices along with combinatorial theranostic devices. The path to understanding a role for DNA nanotechnology in biomedical sciences is being approached carefully and systematically, starting from analyzing the stability and immune‐stimulatory properties of DNA nanostructures in physiological conditions, to estimating their accessibility and application inside cellular and model animal systems. Much remains to be uncovered but the field continues to show promising results toward developing useful biomedical devices. This review discusses some aspects of DNA nanotechnology that makes it a favorable ingredient for creating nanoscale research and biomedical devices and looks at experiments undertaken to determine its stability in vivo. This is presented in conjugation with examples of state‐of‐the‐art developments in biomolecular sensing, imaging, and drug delivery. Finally, some of the major challenges that warrant the attention of the scientific community are highlighted, in order to advance the field into clinically relevant applications.

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Section: Dna Nanostructures As Biomedical Sensorsmentioning

confidence: 99%

The Growing Development of DNA Nanostructures for Potential Healthcare‐Related Applications

Mathur

Medintz

2019

Adv Healthcare Materials

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“…The results showed that the DNA origami devices could detect up to 100 nM of protein with a response time of about 20 min. Strand displacement by the target [88] coupled to the force present in base-stacked DNA duplexes [89] is the most common way to activate the opening of a DNA origami complex. However, these techniques are limited to the nM concentration range and the response times are in the range of minutes or longer.…”

Section: Fluorescence (Förster) Resonance Energy Transfer (Fret)-based Sensorsmentioning

confidence: 99%

DNA Origami as Emerging Technology for the Engineering of Fluorescent and Plasmonic-Based Biosensors

et al. 2020

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DNA nanotechnology is a powerful and promising tool for the development of nanoscale devices for numerous and diverse applications. One of the greatest potential fields of application for DNA nanotechnology is in biomedicine, in particular biosensing. Thanks to the control over their size, shape, and fabrication, DNA origami represents a unique opportunity to assemble dynamic and complex devices with precise and predictable structural characteristics. Combined with the addressability and flexibility of the chemistry for DNA functionalization, DNA origami allows the precise design of sensors capable of detecting a large range of different targets, encompassing RNA, DNA, proteins, small molecules, or changes in physico-chemical parameters, that could serve as diagnostic tools. Here, we review some recent, salient developments in DNA origami-based sensors centered on optical detection methods (readout) with a special emphasis on the sensitivity, the selectivity, and response time. We also discuss challenges that still need to be addressed before this approach can be translated into robust diagnostic devices for bio-medical applications.

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“…For instance, a self-assembled DNA-based nanosystem combining a dumbbell-shaped frame, a cylindrical core and a mobile ring was employed for molecular interrogation being able to evaluate base stacking adhesion and detection of a soluble nucleic acid viral genome mimic. The motion of the nanosystem ring can be induced by a transition between single-stranded DNA to double-stranded DNA which show different rigidity, enabling its use as a mechanism for molecular detection at the ring-frame interface [390].…”

Section: Nanomachinesmentioning

confidence: 99%

Advanced Nanoscale Approaches to Single-(Bio)entity Sensing and Imaging

Neves

Martín‐Yerga

2018

Biosensors

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Individual (bio)chemical entities could show a very heterogeneous behaviour under the same conditions that could be relevant in many biological processes of significance in the life sciences. Conventional detection approaches are only able to detect the average response of an ensemble of entities and assume that all entities are identical. From this perspective, important information about the heterogeneities or rare (stochastic) events happening in individual entities would remain unseen. Some nanoscale tools present interesting physicochemical properties that enable the possibility to detect systems at the single-entity level, acquiring richer information than conventional methods. In this review, we introduce the foundations and the latest advances of several nanoscale approaches to sensing and imaging individual (bio)entities using nanoprobes, nanopores, nanoimpacts, nanoplasmonics and nanomachines. Several (bio)entities such as cells, proteins, nucleic acids, vesicles and viruses are specifically considered. These nanoscale approaches provide a wide and complete toolbox for the study of many biological systems at the single-entity level.

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Programmable DNA Nanosystem for Molecular Interrogation

Cited by 15 publications

References 41 publications

The Growing Development of DNA Nanostructures for Potential Healthcare‐Related Applications

The Growing Development of DNA Nanostructures for Potential Healthcare‐Related Applications

DNA Origami as Emerging Technology for the Engineering of Fluorescent and Plasmonic-Based Biosensors

Advanced Nanoscale Approaches to Single-(Bio)entity Sensing and Imaging

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