Programmable mismatch-fueled high-efficiency DNA signal amplifier

Abstract

Herein, by introducing mismatches, a high-efficiency mismatches-fueled catalytic multiple-arm DNA junction assembly (M-CMDJA) with high-reactivity and high-threshold is developed as a programmable DNA signal amplifier for rapid detection and ultrasensitive...

AgAuS Quantum Dots as a Highly Efficient Near-Infrared Electrochemiluminescence Emitter for the Ultrasensitive Detection of MicroRNA

“…11,12 Therefore, the rapid and accurate detection of trace miRNA is particularly important to achieve early disease diagnosis. Over the years, nucleic acid amplification strategies (rolling circle amplification, 13 hybridization chain reaction, 14 catalytic hairpin assembly, 15 etc.) had been proposed and applied in the fabrication of biosensor for miRNA detection.…”

“…MicroRNA-222 (miRNA-222) played a critical role as a regulator of gene expression in some diseases such as liver cancer and rheumatoid arthritis, , whose expression was associated with toxic heavy metal exposure in the environment. , Therefore, the rapid and accurate detection of trace miRNA is particularly important to achieve early disease diagnosis. Over the years, nucleic acid amplification strategies (rolling circle amplification, hybridization chain reaction, catalytic hairpin assembly, etc.) had been proposed and applied in the fabrication of biosensor for miRNA detection.…”

Ultrasensitive Photocathodic Biosensor Based on the Cu2O/PTB7-Th/PDA+ Composite with Enhanced Photoelectrochemical Performance for the Detection of MicroRNA-375-3p

“…Recently, various DNA amplifying strategies have been reported to enhance the sensitivity of PEC biosensors. − Among them, three-dimensional (3D) DNA walkers have been widely concerned because of the multiple recognition domains and the increased capacity in mimicking complicated biological systems. , Nonetheless, the signal amplification efficiency of 3D DNA walkers is still restricted by the low walking efficiency due to the heterogeneity and local disordered distribution of swinging arms and bundles on the surface. , Thus, it is important to search for an appropriate DNA amplifying strategy that can be combined with 3D DNA walkers to improve the amplification efficiency. DHCR is a modified HCR form, where the traditional hairpin structure fuel chains are replaced by the dumbbell structure DNA .…”

Programmable Molecular Signal Transmission Architecture and Reactant Regeneration Strategy Driven by EXO λ for DNA Circuits

“…In the process of constructing a DNA circuit, DNA strand displacement, as a spontaneous entropy-increasing reaction, offers a reliable technical approach for constructing and regulating entropy-driven molecular circuits. − Drawing inspiration from the design of switching circuits, DNA molecular switches have been developed to enable digital operations of various functions . The precise design of the toehold length in DNA strands allows for the control of hybridization free energy between DNA molecules, realizing analog operations such as weight summation, difference calculation, and multiplication of DNA circuits .…”