Programmable Synthesis of Biobased Materials Using Cell‐Free Systems

Choi, Yun‐Nam; Cho, Namjin; Lee, Kanghun; Gwon, Da‐ae; Lee, Jeong Wook; Lee, Joongoo

doi:10.1002/adma.202203433

Cited by 8 publications

(6 citation statements)

References 308 publications

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“…Cell-free synthesis systems emerge as a promising solution in this scenario [14][15][16][17]. They enable the direct expression of genes from linear DNA without living cells, offering a flexible and controllable platform for swiftly developing enzymatic pathways.…”

Section: Introductionmentioning

confidence: 99%

Cell-Free Synthesis: Expediting Biomanufacturing of Chemical and Biological Molecules

Lee,

Kim

2024

Molecules

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The increasing demand for sustainable alternatives underscores the critical need for a shift away from traditional hydrocarbon-dependent processes. In this landscape, biomanufacturing emerges as a compelling solution, offering a pathway to produce essential chemical materials with significantly reduced environmental impacts. By utilizing engineered microorganisms and biomass as raw materials, biomanufacturing seeks to achieve a carbon-neutral footprint, effectively counteracting the carbon dioxide emissions associated with fossil fuel use. The efficiency and specificity of biocatalysts further contribute to lowering energy consumption and enhancing the sustainability of the production process. Within this context, cell-free synthesis emerges as a promising approach to accelerate the shift towards biomanufacturing. Operating with cellular machinery in a controlled environment, cell-free synthesis offers multiple advantages: it enables the rapid evaluation of biosynthetic pathways and optimization of the conditions for the synthesis of specific chemicals. It also holds potential as an on-demand platform for the production of personalized and specialized products. This review explores recent progress in cell-free synthesis, highlighting its potential to expedite the transformation of chemical processes into more sustainable biomanufacturing practices. We discuss how cell-free techniques not only accelerate the development of new bioproducts but also broaden the horizons for sustainable chemical production. Additionally, we address the challenges of scaling these technologies for commercial use and ensuring their affordability, which are critical for cell-free systems to meet the future demands of industries and fully realize their potential.

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Section: Introductionmentioning

confidence: 99%

Cell-Free Synthesis: Expediting Biomanufacturing of Chemical and Biological Molecules

Lee,

Kim

2024

Molecules

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“…[2] It is more promising by leveraging environmentally friendly and widely adaptable biomaterial DOI: 10.1002/adma.202303457 tools, involving microorganisms and biomolecules, with quantitative yields, fast kinetics, high selectivity, and fidelity for REE separation. [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17] However, there remain significant challenges for biomaterials in industry application due to their inefficiency in extracting high-performance REE materials from ores. Therefore, developing a new generation of biosynthetic materials for agile biofoundry is necessary to obtain high-value utilization of REEs.…”

Section: Introductionmentioning

confidence: 99%

The Construction of a Microbial Synthesis System for Rare Earth Enrichment and Material Applications

et al. 2023

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Rare earth materials play an irreplaceable role in biomedical and high technology fields. However, typical mining and extraction approaches to rare earth elements (REEs) often lead to severe environmental problems and resource wastage due to the involvement of hazardous chemicals. Although biomining shows elegant alternatives, there are still grand challenges to sustainably isolate and recover REEs in nature because of insufficient metal‐extracting microbes and RE‐scavenging macromolecular tools. To obtain high‐performance rare earth materials directly from rare earth ore, a new generation of biological synthesis strategies needs to be developed for the efficient preparation of REEs. The microbial synthesis system established here has achieved active biomanufacturing of high‐purity rare earth products. Further, through employing robust affinity columns bioconjugated with structurally engineered proteins, outstanding separation of Eu/Lu and Dy/La is acquired with the purity of 99.9% (Eu), 97.1% (La), and 92.7% (Dy). More importantly, in situ one‐pot synthesis of lanthanide‐dependent methanol dehydrogenase is well harnessed and exclusively adsorbs La, Ce, Pr, and Nd in RE tailing for advanced biocatalysis, indicating high value‐added application. Therefore, this novel biosynthetic platform provides an insightful roadmap to expand the scope of chassis engineering in terms of biofoundry and to manufacture valuable bioproducts related to REEs.

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“…10,11 Despite the advantageous features, the whole cell-based technology has long been plagued by concerns, including cell culturing, transmembrane transportation, and biosafety, which preclude the widespread application. 4,12 To circumvent these limitations, cell-free transcription-translation (TX-TL) systems have been developed to achieve the same function in a biosafe format. 12 For example, by coupling the bio-circuit with the in vitro TX-TL system, a cell-free biosensor can detect the Ebola virus with reduced cost and time.…”

mentioning

confidence: 99%

“…4,12 To circumvent these limitations, cell-free transcription-translation (TX-TL) systems have been developed to achieve the same function in a biosafe format. 12 For example, by coupling the bio-circuit with the in vitro TX-TL system, a cell-free biosensor can detect the Ebola virus with reduced cost and time. 13 The components of the cell-free system can be freeze-dried for field deployable applications, which has an intuitive appeal for the detection of pathogens, especially in the epidemic of SARS-CoV-2.…”

mentioning

confidence: 99%

“…The aspiration of building artificial molecular machines has motivated synthetic biologists to capitalize on living cells as efficient synthetic tools. − The resulting development of cell-based synthesis that harnesses cells’ natural ability to sense the environment and produce biobased materials has revolutionized the fields of biosensing, − bioproduction, , and biocomputing. , Despite the advantageous features, the whole cell-based technology has long been plagued by concerns, including cell culturing, transmembrane transportation, and biosafety, which preclude the widespread application. , To circumvent these limitations, cell-free transcription-translation (TX-TL) systems have been developed to achieve the same function in a biosafe format . For example, by coupling the bio-circuit with the in vitro TX-TL system, a cell-free biosensor can detect the Ebola virus with reduced cost and time .…”

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confidence: 99%

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Accelerating Cell-Free Biosensors by Entropy-Driven Assembly of Transcription Templates

et al. 2023

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Due to its high efficiency and selectivity, cell-free biosynthesis has found broad utility in the fields of bioproduction, environment monitoring, and disease diagnostics. However, the practical application is limited by its low productivity. Here, we introduce the entropy-driven assembly of transcription templates as dynamic amplifying modules to accelerate the cell-free transcription process. The catalytic DNA circuit with high sensitivity and enzyme-free format contributes to the production of large amounts of transcription templates, drastically accelerating the as-designed cell-free transcription system without interference from multiple enzymes. The proposed approach was successfully applied to the ultrasensitive detection of SARS-CoV-2, improving the sensitivity by 3 orders of magnitude. Thanks to the high programmability and diverse light-up RNA pairs, the method can be adapted to multiplexing detection, successfully demonstrated by the analysis of two different sites of the SARS-CoV-2 gene in parallel. Further, the flexibility of the entropy-driven circuit enables a dynamic responding range by tuning the circuit layers, which is beneficial for responding to targets with different concentration ranges. The strategy was also applied to the analysis of clinical samples, providing an alternative for sensitively detecting the current SARS-CoV-2 RNA that quickly mutates.

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Programmable Synthesis of Biobased Materials Using Cell‐Free Systems

Cited by 8 publications

References 308 publications

Cell-Free Synthesis: Expediting Biomanufacturing of Chemical and Biological Molecules

Cell-Free Synthesis: Expediting Biomanufacturing of Chemical and Biological Molecules

The Construction of a Microbial Synthesis System for Rare Earth Enrichment and Material Applications

Accelerating Cell-Free Biosensors by Entropy-Driven Assembly of Transcription Templates

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