Programmed cell death 4 and BCR-ABL fusion gene expression are negatively correlated in chronic myeloid leukemia

Abstract

Abstract. Programmed cell death 4 (PDCD4) is a tumor suppressor that inhibits carcinogenesis, tumor progression and invasion by preventing gene transcription and translation. Downregulation of PDCD4 expression has been identified in multiple types of human cancer, however, to date, the function of PDCD4 in leukemia has not been investigated. In the present study, PDCD4 mRNA and protein expression was investigated in 50 patients exhibiting various phases of chronic myeloid leukemia (CML) and 20 healthy individuals by reverse transcription-quantitative polymerase chain reaction and western blot analysis. PDCD4 expression and cell proliferation was also investigated following treatment with the tyrosine kinase inhibitor, imatinib, in K562 cells. The results demonstrated that PDCD4 mRNA and protein expression was decreased in all CML samples when compared with healthy controls, who expressed high levels of PDCD4 mRNA and protein. No significant differences in PDCD4 expression were identified between chronic phase, accelerated phase and blast phase CML patients. In addition, PDCD4 expression was negatively correlated with BCR-ABL gene expression (r=-0.6716; P<0.001). Furthermore, K562 cells treated with imatinib exhibited significantly enhanced PDCD4 expression. These results indicate that downregulation of PDCD4 expression may exhibit a critical function in the progression and malignant proliferation of human CML.

Overexpression of miR-519d in lung adenocarcinoma inhibits cell proliferation and invasion via the association of eIF4H

“…Recently, the mechanisms involved in the invasion and metastasis processes of the specific gene expression and regulation pathways have been widely explored in different cancers, such as colon cancer, esophageal cancer, breast cancer, and chronic myeloid leukemia. [24][25][26][27][28] Due to findings from recent studies, many genetic factors, including lncRNA, 29 miRNA 30 as well as matrix metalloproteinase (MMP) family members, 31 have become associated with the metastasis of lung cancer. MiR-519d is reported to be downregulated in lung cancer.…”

Expression and function of Pdcd4 in mouse endometrium during early pregnancy

“…PDCD4 is a highly conserved protein with two basic domains at the N/C-terminus and is known to inhibit carcinogenesis, tumour progression and invasion by preventing gene transcription and translation (Zhang et al 2016). PDCD4 can also regulate several biological function via several factors and pathways, such as S6K1, microRNA-21 (miR-21), mTOR and pAkt (Lankat-Buttgereit & Goke 2009).…”