Programmed cell death in salivary glands of Drosophila arizonae and Drosophila mulleri

Ianella, P.; Azeredo-Oliveira, Maria Tercília Vilela de; Itoyama, Mary Massumi

doi:10.4238/vol7-2gmr412

Cited by 9 publications

(8 citation statements)

References 39 publications

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“…The accumulation of clumps of condensed chromatin frequently attached to the inner surface of the nuclear membrane has been reported to be one of the most common morphological indicators of ongoing apoptosis (Kerr et al 1972;Wyllie 1980). Moreover, Ianella et al (2008) have reported that the lysosomal bodies around the nuclei tend to degrade even before fusing with the nuclei. The staining of PV tissues in the present investigation also agrees with these findings.…”

Section: Discussionmentioning

confidence: 98%

Modes of cell death in the pupal perivisceral fat body tissue of the silkworm Bombyx mori L.

2009

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Cell death is a scheduled event during animal development and tissue turnover. Here, we affirm the presence of two major pathways of programmed cell death (PCD), viz. apoptotic and autophagic cell death, in the disintegrated pupal perivisceral (PV) fat body during pupal-adult metamorphosis. The acridine orange (a vital stain for apoptosis) staining pattern and DNA fragmentation assay have revealed the exact day (6th day of the pupal stage) of disintegration in the PV fat body as represented by chromatin condensation and DNA laddering. Electron microscopy and scanning electron microscopy have demonstrated the presence of cytoplasmic budding and giant autophagic vacuoles and the low numbers of mitochondria, all of which are attributes of autophagic cell death. Immunoblot analysis of proteosomal subunits 20S and 26S has established the involvement of proteolytic activity during PCD of PV tissue. Lysosomal participation during the PCD of PV tissues has been confirmed by the elevated level of the marker enzyme, acid phosphatase, which is distinct on day 6 of the pupal period. The results of the present study have thus ascertained the co-existence of both autophagic and apoptotic cell death in PV fat body tissue.

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Section: Discussionmentioning

confidence: 98%

Modes of cell death in the pupal perivisceral fat body tissue of the silkworm Bombyx mori L.

2009

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“…Apoptosis is identified by morphological changes like condensation of cytoplasm and chromatin, breakdown of nuclear membrane, membrane blebbing and, in epithelial cells, loss of membrane polarity and disruption of cell junction proteins (Jänicke et al 1998;Atencia et al 2000;Ianella et al 2008). The canonical apoptotic pathway is evolutionarily conserved in invertebrates as well as in vertebrates (Aravind et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Non-apoptotic function of apoptotic proteins in the development of Malpighian tubules of Drosophila melanogaster

Tapadia

Gautam

2011

J Biosci

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Drosophila metamorphosis is characterized by the histolysis of larval structures by programmed cell death, which paves the way for the establishment of adult-specific structures under the influence of the steroid hormone ecdysone. Malpighian tubules function as an excretory system and are one of the larval structures that are not destroyed during metamorphosis and are carried over to adulthood. The pupal Malpighian tubules evade destruction in spite of expressing apoptotic proteins, Reaper, Hid, Grim, Dronc and Drice. Here we show that in the Malpighian tubules expression of apoptotic proteins commences right from embryonic development and continues throughout the larval stages. Overexpression of these proteins in the Malpighian tubules causes larval lethality resulting in malformed tubules. The number and regular organization of principal and stellate cells of Malpighian tubules is disturbed, in turn disrupting the physiological functioning of the tubules as well. Strikingly, the localization of beta-tubulin, F-actin and Disclarge (Dlg) is also disrupted. These results suggest that the apoptotic proteins could be having non-apoptotic function in the development of Malpighian tubules.

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“…In D. variabilis and B. microplus , cell death is induced by apoptosis [5–8, 37, 38]. Our study showed that the apoptosis in salivary glands occurred at 3–4 days after attachment, which differs from that in R. sanguineus [9].…”

Section: Discussionmentioning

confidence: 79%

Caspase-1 participates in apoptosis of salivary glands in Rhipicephalus haemaphysaloides

Zhou

Cao

et al. 2017

Parasites Vectors

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BackgroundTicks are among the most harmful vectors worldwide. Their salivary glands play essential roles in blood-feeding and pathogen transmission and undergo apoptosis after feeding. Although it was previously reported that salivary degeneration in ixodid ticks is in response to hormonal stimulation, questions still exist with the underlying mechanisms of salivary gland apoptosis.MethodsSalivary glands of Rhipicephalus haemaphysaloides were collected from 1 to 7 days after attachment to the host. TUNEL and Annexin V assays were used to check apoptosis during this time. To confirm the role of caspase-1, RNA interference was used to silence its expression, and the dynamic changes of associated cysteine proteases were also shown by quantitative real time PCR and western blot, while TUNEL and Annexin V assays were used to confirm apoptosis.ResultsIn the present study, apoptosis of salivary glands in R. haemaphysaloides occurred 3 or 4 days after attachment to the host as determined by TUNEL and Annexin V assays. The expression of caspase-1 increased at 5–7 days. When the latter was silenced by RNA interference, apoptosis in the salivary glands was delayed. While there seemed to be another form of cell death in salivary glands of ticks, such occurrence may be caused by compensatory autophagy which involved autophagy-related gene 4D.ConclusionsThis study describes the apoptosis of salivary glands in R. haemaphysaloides and the dynamic changes in cysteine proteases in this activity. Cysteine proteases were involved in this process, especially caspase-1. Caspase-1 participated in the apoptosis of salivary glands.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-017-2161-1) contains supplementary material, which is available to authorized users.

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Programmed cell death in salivary glands of Drosophila arizonae and Drosophila mulleri

Cited by 9 publications

References 39 publications

Modes of cell death in the pupal perivisceral fat body tissue of the silkworm Bombyx mori L.

Modes of cell death in the pupal perivisceral fat body tissue of the silkworm Bombyx mori L.

Non-apoptotic function of apoptotic proteins in the development of Malpighian tubules of Drosophila melanogaster

Caspase-1 participates in apoptosis of salivary glands in Rhipicephalus haemaphysaloides

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