Programmed gene expression change in mouse skin after ultraviolet radiation damage

Abstract

Ultraviolet (UV) radiation is a major cause of skin damage and carcinogenesis. Here, we systematically analyse the acute gene expression change in skin in vivo after UV exposure, aiming to establish the common C57BL/6 mouse strain as a convenient model for future pathological research and drug discovery. The back fur of C57BL/6 mice was depilated, and a mixed UV light source was used to irradiate the skin. Full‐thickness skin samples were collected at 0, 0.5, 2, 6, 12 and 24 h. Total RNAs were extracted and subjected to RNA sequencing analysis. We found that the gene expression change in mouse skin is highly similar to previous reports in human skin. These include down‐regulation of differentiation‐related genes and extracellular matrix genes, and up‐regulation of cytokine/chemokine genes. An early wave of activator protein 1 (AP‐1) expression is induced, whereas activation of the p53 pathway is not significant. The impact of the AP‐1 transcription factors and the antioxidant tea polyphenols is discussed. The analysis of acute gene expression change in skin after UV irradiation provides a starting point to investigate how the skin responds to genotoxic stress.

Research on Permeation Influencing Factors of Cosmetics UV Filters and Improve In Vitro Permeation Tests (IVPTs)

“…The ideal UV absorber should be safe, bland, and have excellent broad-spectrum protection, photostability, chemical stability, and compatibility. However, in recent years, studies have found that some UV absorbers can penetrate the skin [2] and cause skin damage by causing photosensitivity, phototoxic reactions, and skin irritation [3]. In 2019, Matta et al [2] evaluated whether the UV absorber components (avobenzone, oxybenzone, octocrylene, and ecamsule) of four commercial sunscreen products could be absorbed into the body's circulation.…”