Programming effects of an early life diet containing large phospholipid-coated lipid globules are transient under continuous exposure to a high-fat diet

Ronda, Onne; Heijning, Bert J. M. van de; Bruin, Alain de; Jurdzinski, Angelika; Kuipers, Folkert; Verkade, Henkjan J.

doi:10.1017/s0007114519002083

Cited by 10 publications

(29 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the moderate WSD challenge (22% energy from fat) used in this study is mild in comparison with the often-used models of high fat diet (HFD) exposure (40%-60% energy from fat) [50,51], animals still seemed to develop a rather obesogenic phenotype, likely related to the duration of WSD exposure in the current study (~27 weeks). This is supported by a recent study showing that programming effects of N-IMF on fat deposition are transient under continuous exposure to HFD [52]. These results warrant further studies to solidify findings on programming effects of ES and N-IMF on metabolism earlier in life.…”

Section: Discussionsupporting

confidence: 70%

The Effects of Early Life Stress, Postnatal Diet Modulation, and Long-Term Western-Style Diet on Later-Life Metabolic and Cognitive Outcomes

et al. 2020

View full text Add to dashboard Cite

Early life stress (ES) increases the risk to develop metabolic and brain disorders in adulthood. Breastfeeding (exclusivity and duration) is associated with improved metabolic and neurocognitive health outcomes, and the physical properties of the dietary lipids may contribute to this. Here, we tested whether early life exposure to dietary lipids mimicking some physical characteristics of breastmilk (i.e., large, phospholipid-coated lipid droplets; Concept Nuturis® infant milk formula (N-IMF)), could protect against ES-induced metabolic and brain abnormalities under standard circumstances, and in response to prolonged Western-style diet (WSD) in adulthood. ES was induced by exposing mice to limited nesting material from postnatal day (P) 2 to P9. From P16 to P42, male offspring were fed a standard IMF (S-IMF) or N-IMF, followed by either standard rodent diet (SD) or WSD until P230. We then assessed body composition development, fat mass, metabolic hormones, hippocampus-dependent cognitive function, and neurogenesis (proliferation and survival). Prolonged WSD resulted in an obesogenic phenotype at P230, which was not modulated by previous ES or N-IMF exposure. Nevertheless, ES and N-IMF modulated the effect of WSD on neurogenesis at P230, without affecting cognitive function, highlighting programming effects of the early life environment on the hippocampal response to later life challenges at a structural level.

show abstract

Section: Discussionsupporting

confidence: 70%

The Effects of Early Life Stress, Postnatal Diet Modulation, and Long-Term Western-Style Diet on Later-Life Metabolic and Cognitive Outcomes

et al. 2020

View full text Add to dashboard Cite

show abstract

“…At PN42, corresponding with 26 days of either eIMF or cIMF feeding, plasma cytokines and bile acid metabolism were similar between groups. These data render it unlikely that the long-term effects of eIMF on body weight and fat mass gain following a Western-style diet 5,14 are mechanistically related to these parameters. At the end of the IMF feeding period, prior to a (high fat) dietary challenge, we do see significantly higher levels of hepatic proteins involved in fatty acid oxidation and the TCA cycle.…”

Section: Discussionmentioning

confidence: 97%

Effects of an early life diet containing large phospholipid-coated lipid globules on hepatic lipid metabolism in mice

Ronda

Heijning

Martini

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

We recently reported that feeding mice in their early life a diet containing a lipid structure more similar to human milk (eIMF, Nuturis) results in lower body weights and fat mass gain upon high fat feeding in later life, compared to control (cIMF). To understand the underlying mechanisms, we now explored parameters possibly involved in this long-term effect. Male C57BL/6JOlaHsd mice, fed rodent diets containing eIMF or cIMF from postnatal (PN) day 16–42, were sacrificed at PN42. Hepatic proteins were measured using targeted proteomics. Lipids were assessed by LC–MS/MS (acylcarnitines) and GC-FID (fatty-acyl chain profiles). Early life growth and body composition, cytokines, and parameters of bile acid metabolism were similar between the groups. Hepatic concentrations of multiple proteins involved in β-oxidation (+ 17%) the TCA cycle (+ 15%) and mitochondrial antioxidative proteins (+ 28%) were significantly higher in eIMF versus cIMF-fed mice (p < 0.05). Hepatic l-carnitine levels, required for fatty acid uptake into the mitochondria, were higher (+ 33%, p < 0.01) in eIMF-fed mice. The present study indicates that eIMF-fed mice have higher hepatic levels of proteins involved in fatty acid metabolism and oxidation. We speculate that eIMF feeding programs the metabolic handling of dietary lipids.

show abstract

“…Lean and fat mass were quantified by time-domain nuclear magnetic resonance (LF90II, Bruker Optics, Billerica, MA), not requiring fasting or anesthesia as described [9]. Simultaneously, body weights were recorded.…”

Section: Methodsmentioning

confidence: 99%

Spontaneous liver disease in wild-type C57BL/6JOlaHsd mice fed semisynthetic diet

Ronda

Heijning

Bruin

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Mouse models are frequently used to study mechanisms of human diseases. Recently, we observed a spontaneous bimodal variation in liver weight in C57BL/6JOlaHsd mice fed a semisynthetic diet. We now characterized the spontaneous variation in liver weight and its relationship with parameters of hepatic lipid and bile acid (BA) metabolism. In male C57BL/6JOlaHsd mice fed AIN-93G from birth to postnatal day (PN)70, we measured plasma BA, lipids, Very low-density lipoprotein (VLDL)-triglyceride (TG) secretion, and hepatic mRNA expression patterns. Mice were sacrificed at PN21, PN42, PN63 and PN70. Liver weight distribution was bimodal at PN70. Mice could be subdivided into two nonoverlapping groups based on liver weight: 0.6 SD 0.1 g (approximately one-third of mice, small liver; SL), and 1.0 SD 0.1 g (normal liver; NL; p<0.05). Liver histology showed a higher steatosis grade, inflammation score, more mitotic figures and more fibrosis in the SL versus the NL group. Plasma BA concentration was 14-fold higher in SL (p<0.001). VLDL-TG secretion rate was lower in SL mice, both absolutely (−66%, p<0.001) and upon correction for liver weight (−44%, p<0.001). Mice that would later have the SL-phenotype showed lower food efficiency ratios during PN21-28, suggesting the cause of the SL phenotype is present at weaning (PN21). Our data show that approximately one-third of C57BL/6JOlaHsd mice fed semisynthetic diet develop spontaneous liver disease with aberrant histology and parameters of hepatic lipid, bile acid and lipoprotein metabolism. Study designs involving this mouse strain on semisynthetic diets need to take the SL phenotype into account. Plasma lipids may serve as markers for the identification of the SL phenotype.

show abstract

Programming effects of an early life diet containing large phospholipid-coated lipid globules are transient under continuous exposure to a high-fat diet

Cited by 10 publications

References 34 publications

The Effects of Early Life Stress, Postnatal Diet Modulation, and Long-Term Western-Style Diet on Later-Life Metabolic and Cognitive Outcomes

The Effects of Early Life Stress, Postnatal Diet Modulation, and Long-Term Western-Style Diet on Later-Life Metabolic and Cognitive Outcomes

Effects of an early life diet containing large phospholipid-coated lipid globules on hepatic lipid metabolism in mice

Spontaneous liver disease in wild-type C57BL/6JOlaHsd mice fed semisynthetic diet

Contact Info

Product

Resources

About