Progress advances in the production of bio-sourced methionine and its hydroxyl analogues

François, Jean Marie

doi:10.1016/j.biotechadv.2023.108259

Cited by 13 publications

(3 citation statements)

References 127 publications

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“…55 Except for some endosymbiotic species, almost all microorganisms have a L-methionine biosynthetic pathway, although their enzymes and encoding genes can be different. 36,56 L-Methionine is synthesized through branched pathways (Figure 1A). The first committed step in the L-methionine biosynthesis is the activation of L-homoserine by succinylation or acetylation.…”

Section: Sam Biosynthetic Pathwaymentioning

confidence: 99%

“…It has been shown that insufficient l -methionine availability is one of the bottlenecks in SAM biosynthesis. , This issue can be resolved by enhancing l -methionine biosynthesis and/or importing exogenous l -methionine from the culture medium . Except for some endosymbiotic species, almost all microorganisms have a l -methionine biosynthetic pathway, although their enzymes and encoding genes can be different. , l -Methionine is synthesized through branched pathways (Figure A). The first committed step in the l -methionine biosynthesis is the activation of L-homoserine by succinylation or acetylation.…”

Section: Sam Biosynthetic Pathwaymentioning

confidence: 99%

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Improving Microbial Cell Factory Performance by Engineering SAM Availability

Lv,

Chang,

Zhang

et al. 2024

J. Agric. Food Chem.

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Methylated natural products are widely spread in nature. S-Adenosyl-L-methionine (SAM) is the secondary abundant cofactor and the primary methyl donor, which confer natural products with structural and functional diversification. The increasing demand for SAM-dependent natural products (SdNPs) has motivated the development of microbial cell factories (MCFs) for sustainable and efficient SdNP production. Insufficient and unsustainable SAM availability hinders the improvement of SdNP MCF performance. From the perspective of developing MCF, this review summarized recent understanding of de novo SAM biosynthesis and its regulatory mechanism. SAM is just the methyl mediator but not the original methyl source. Effective and sustainable methyl source supply is critical for efficient SdNP production. We compared and discussed the innate and relatively less explored alternative methyl sources and identified the one involving cheap one-carbon compound as more promising. The SAM biosynthesis is synergistically regulated on multilevels and is tightly connected with ATP and NAD(P)H pools. We also covered the recent advancement of metabolic engineering in improving intracellular SAM availability and SdNP production. Dynamic regulation is a promising strategy to achieve accurate and dynamic fine-tuning of intracellular SAM pool size. Finally, we discussed the design and engineering constraints underlying construction of SAM-responsive genetic circuits and envisioned their future applications in developing SdNP MCFs.

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Section: Sam Biosynthetic Pathwaymentioning

confidence: 99%

Section: Sam Biosynthetic Pathwaymentioning

confidence: 99%

Improving Microbial Cell Factory Performance by Engineering SAM Availability

Lv,

Chang,

Zhang

et al. 2024

J. Agric. Food Chem.

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“…[24][25][26] As an example, α-hydroxy-γbutyrolactone (HBL) is a α-hydroxy functionalized γ-lactone that can be obtained from glucose using a biological synthetic route. [27][28][29] Among the biobased polymer family, [30][31][32][33] poly(lactide) (PLA) is a widely marketed polymer derived from renewable resources such as corn starch and potato. 34,35 Despite its numerous advantages, the degradation of PLA in the environment remains a challenge due to its resistance to microorganisms attack.…”

Section: Introductionmentioning

confidence: 99%

Ring‐opening polymerization of l‐lactide in the presence of α‐hydroxy‐γ‐butyrolactone

Pascouau,

Carlotti,

Cramail

et al. 2024

Polymers for Advanced Techs

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Among the polymer families, aliphatic polyesters stand out from this category thanks to their degradable and biocompatible properties. In particular, the γ‐lactones differ from other lactones by yielding polyesters that can be depolymerized back to the monomer and offer the advantage of counting various biobased monomers. As an example, α‐hydroxy‐γ‐butyrolactone (HBL) is a hydroxy‐functionalized monomer that can be obtained by a biological synthetic route from glucose. In this article, the ring‐opening copolymerization (ROCP) of HBL and l‐lactide (LLA) using t‐BuP4 as catalyst is investigated. The copolymerizations were conducted within a temperature range of 5–100°C, affording monomer conversions exceeding 80%. The characterization of the copolyesters revealed a branched structure consisting of different HBL patterns, including cyclic, linear, and branched motives. Performing a kinetic study of the copolymerization at room temperature provided a deeper understanding of the mechanism. By modulating the reaction parameters, copolymers of low molar masses with an HBL content of up to 33% were synthesized. High molar mass LLA/HBL‐based copolyesters, with up to 290,000 g/mol, were synthesized by a straightforward chain coupling reaction with a diisocyanate.

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